{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T20:30:26Z","timestamp":1790454626819,"version":"4.1.0"},"reference-count":49,"publisher":"Oxford University Press (OUP)","issue":"4","license":[{"start":{"date-parts":[[2021,3,8]],"date-time":"2021-03-08T00:00:00Z","timestamp":1615161600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"name":"Government of Santa Fe, Argentina","award":["1010-10-14"],"award-info":[{"award-number":["1010-10-14"]}]},{"name":"Government of Santa Fe, Argentina","award":["1010-031-17"],"award-info":[{"award-number":["1010-031-17"]}]},{"name":"Agencia Nacional de Ciencia y T\u00e9cnica","award":["ANCyT PICT2014-1127"],"award-info":[{"award-number":["ANCyT PICT2014-1127"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2021,3,24]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Decreased fertility is becoming an important social and medical problem and the male factor is involved in at least half of infertility cases. Since conventional semen analysis provides limited prediction of male fertility; in this work, we evaluated the potential use of seminal small RNAs (sRNA) as markers of semen quality in ART. Our bioinformatic analyses of available sRNA-seq databases showed that the most abundant sRNA species in seminal plasma of normozoospermic men are tRNA-derived fragments (tRFs), a novel class of regulatory sRNAs. These molecules not only exert their function within cells but also are released into the extracellular environment where they could carry out signaling functions. To evaluate whether the assessment of seminal tRFs in normozoospermic men has a predictive value for the clinical outcome in ART, we performed a prospective study with couples who underwent ICSI cycles with donated oocytes. The results obtained demonstrated that levels of 5\u2019tRF-Glu-CTC, 5\u2019tRF-Lys-CTT, and 5\u2019tRF-Gly-GCC are significantly elevated in seminal samples from cases with repeated failed ICSI cycles, suggesting a potential association between increased seminal tRFs and unexplained male infertility. Interestingly, these tRFs showed a negative association with seminal testosterone, highlighting their involvement in male endocrinology. Our findings also suggest that tRFs could play a role in modulating male reproductive function in response to physiological stress since they showed significant associations with the levels of sperm DNA fragmentation in couples that achieved pregnancy but not in cases with failed ICSI cycles where seminal cortisol levels correlate with sperm quality.<\/jats:p>","DOI":"10.1093\/molehr\/gaab017","type":"journal-article","created":{"date-parts":[[2021,3,3]],"date-time":"2021-03-03T20:18:56Z","timestamp":1614802736000},"source":"Crossref","is-referenced-by-count":22,"title":["Levels of seminal tRNA-derived fragments from normozoospermic men correlate with the success rate of ART"],"prefix":"10.1093","volume":"27","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8151-5089","authenticated-orcid":false,"given":"Julieta B","family":"Grosso","sequence":"first","affiliation":[{"name":"Institute of Clinical and Experimental Immunology of Rosario (IDICER CONICET-UNR), Rosario, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luciana","family":"Zoff","sequence":"additional","affiliation":[{"name":"Institute of Clinical and Experimental Immunology of Rosario (IDICER CONICET-UNR), Rosario, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4642-6555","authenticated-orcid":false,"given":"Karina L","family":"Calvo","sequence":"additional","affiliation":[{"name":"Laboratorios Biol\u00f3gicos PROAR, Rosario, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mar\u00eda Bel\u00e9n","family":"Maraval","sequence":"additional","affiliation":[{"name":"Institute of Clinical and Experimental Immunology of Rosario (IDICER CONICET-UNR), Rosario, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mariana","family":"Perez","sequence":"additional","affiliation":[{"name":"Laboratorios Biol\u00f3gicos PROAR, Rosario, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marines","family":"Carbonaro","sequence":"additional","affiliation":[{"name":"Laboratorios Biol\u00f3gicos PROAR, Rosario, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claudia","family":"Brignardello","sequence":"additional","affiliation":[{"name":"Laboratorios Biol\u00f3gicos PROAR, Rosario, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carlos","family":"Morente","sequence":"additional","affiliation":[{"name":"Laboratorios Biol\u00f3gicos PROAR, Rosario, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2844-5485","authenticated-orcid":false,"given":"Silvana V","family":"Spinelli","sequence":"additional","affiliation":[{"name":"Institute of Clinical and Experimental Immunology of Rosario (IDICER CONICET-UNR), Rosario, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2021,3,8]]},"reference":[{"key":"2021042417493847700_gaab017-B1","doi-asserted-by":"crossref","first-page":"e13503","DOI":"10.1111\/and.13503","article-title":"MicroRNA signature in spermatozoa and seminal plasma of proven fertile men and in testicular tissue of men with obstructive azoospermia","volume":"52","author":"Abu-Halima","year":"2020","journal-title":"Andrologia"},{"key":"2021042417493847700_gaab017-B2","doi-asserted-by":"crossref","first-page":"1067","DOI":"10.1016\/j.fertnstert.2018.06.039","article-title":"International Committee for Monitoring Assisted Reproductive Technology: world report on assisted reproductive technology, 2011","volume":"110","author":"Adamson","year":"2018","journal-title":"Fertil Steril"},{"key":"2021042417493847700_gaab017-B3","doi-asserted-by":"crossref","first-page":"173","DOI":"10.1016\/bs.acr.2017.06.007","article-title":"Role of 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