{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T23:28:44Z","timestamp":1787009324106,"version":"build-2736575974"},"reference-count":81,"publisher":"Oxford University Press (OUP)","issue":"7","license":[{"start":{"date-parts":[[2023,5,26]],"date-time":"2023-05-26T00:00:00Z","timestamp":1685059200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81703193"],"award-info":[{"award-number":["81703193"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32002178"],"award-info":[{"award-number":["32002178"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2023,6,30]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Chromium (Cr) and its compounds are closely associated with individuals\u2019 lives and extensively used in industry. Excessive exposure to hexavalent chromium (Cr(VI)) induces oxidative damage of various organs including the testes, posing a serious threat to male reproductive fitness. As an endogenous antioxidant, melatonin holds potent antioxidative and anti-inflammatory properties, becoming a potential candidate for treatment of a variety of diseases, including reproductive disorders. Here, by using a mouse model, we systematically assessed Cr(VI)-induced damage to male fertility as well as the preventive role of melatonin. We analyzed the histology and pathology of the testis and epididymis, the density, viability, and malformation of caudal epididymal sperm, the proliferative activity and apoptosis of various spermatogenic subtypes and Sertoli cells, as well as the fertility of mice at five timepoints within one cycle of spermatogenesis (Days 0, 14, 21, 28, and 35) post 14\u2009days of Cr(VI) and\/or melatonin intraperitoneal injection. We identified that the testicular damage caused by Cr(VI) persisted to Day 21 after administration and then started to be alleviated, with clear alleviation on Day 35. Pretreatment with melatonin evidently reduced Cr(VI)-induced testicular damage and accelerated spermatogenic restoration, generating an almost normal phenotype on Day 35. Melatonin pretreatment also retained the sperm quality at all time points investigated. Moreover, melatonin to some extent preserved the fertility of Cr(VI)-treated mice without apparent side effects. The findings shed light on the future clinical application of melatonin as a therapeutic agent for environmental heavy metal toxicant-induced male subfertility or infertility.<\/jats:p>","DOI":"10.1093\/molehr\/gaad020","type":"journal-article","created":{"date-parts":[[2023,5,25]],"date-time":"2023-05-25T09:57:42Z","timestamp":1685008662000},"source":"Crossref","is-referenced-by-count":20,"title":["Systematic assessment of hexavalent chromium-induced damage to male fertility and the preventive role of melatonin: a longitudinal study from the translational point of view"],"prefix":"10.1093","volume":"29","author":[{"given":"Tianjiao","family":"Li","sequence":"first","affiliation":[{"name":"Key Laboratory for Animal Genetics, Breeding and Reproduction of Shaanxi Province, College of Animal Science and Technology, Northwest A&F University , Yangling, Shaanxi, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yinghua","family":"Lv","sequence":"additional","affiliation":[{"name":"Shaanxi Key Laboratory of Natural Products & Chemical Biology, College of Chemistry & Pharmacy, Northwest A&F University , Yangling, Shaanxi, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhili","family":"Wu","sequence":"additional","affiliation":[{"name":"Key Laboratory for Animal Genetics, Breeding and Reproduction of Shaanxi Province, College of Animal Science and Technology, Northwest A&F University , Yangling, Shaanxi, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ming","family":"Guo","sequence":"additional","affiliation":[{"name":"Key Laboratory for Animal Genetics, Breeding and Reproduction of Shaanxi Province, College of Animal Science and Technology, Northwest A&F University , Yangling, Shaanxi, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruifang","family":"Liu","sequence":"additional","affiliation":[{"name":"Key Laboratory for Animal Genetics, Breeding and Reproduction of Shaanxi Province, College of Animal Science and Technology, Northwest A&F University , Yangling, Shaanxi, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2912-8943","authenticated-orcid":false,"given":"Wenxian","family":"Zeng","sequence":"additional","affiliation":[{"name":"Key Laboratory for Animal Genetics, Breeding and Reproduction of Shaanxi Province, College of Animal Science and Technology, Northwest A&F University , Yangling, Shaanxi, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0152-2671","authenticated-orcid":false,"given":"Yi","family":"Zheng","sequence":"additional","affiliation":[{"name":"Key Laboratory for Animal Genetics, Breeding and Reproduction of Shaanxi Province, College of Animal Science and Technology, Northwest A&F University , Yangling, Shaanxi, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2023,5,26]]},"reference":[{"key":"2023063013481218000_gaad020-B1","first-page":"137","article-title":"Germ-cell sensitivity in mammals","volume":"109","author":"Adler","year":"1982","journal-title":"Prog Clin Biol Res"},{"key":"2023063013481218000_gaad020-B2","doi-asserted-by":"crossref","first-page":"17370","DOI":"10.1002\/jcp.28357","article-title":"Melatonin supplementation during in vitro maturation of oocyte enhances subsequent development of bovine cloned embryos","volume":"234","author":"An","year":"2019","journal-title":"J Cell Physiol"},{"key":"2023063013481218000_gaad020-B3","doi-asserted-by":"crossref","first-page":"411","DOI":"10.2166\/wst.2019.060","article-title":"Alleviation of hexavalent chromium by using microorganisms: insight into the strategies and complications","volume":"79","author":"Bhattacharya","year":"2019","journal-title":"Water Sci Technol"},{"key":"2023063013481218000_gaad020-B4","doi-asserted-by":"crossref","first-page":"225","DOI":"10.1080\/00039896.1991.9937453","article-title":"Chromium in biological samples from low-level exposed stainless steel and mild steel welders","volume":"46","author":"Bonde","year":"1991","journal-title":"Arch Environ Health"},{"key":"2023063013481218000_gaad020-B5","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1002\/aja.1001910104","article-title":"Development of Sertoli cell junctional specializations and the distribution of the tight-junction-associated protein ZO-1 in the mouse testis","volume":"191","author":"Byers","year":"1991","journal-title":"Am J Anat"},{"key":"2023063013481218000_gaad020-B6","doi-asserted-by":"crossref","first-page":"113119","DOI":"10.1016\/j.biopha.2022.113119","article-title":"Mechanism of chromium-induced toxicity in lungs, liver, and kidney and their ameliorative agents","volume":"151","author":"Chakraborty","year":"2022","journal-title":"Biomed Pharmacother"},{"key":"2023063013481218000_gaad020-B7","doi-asserted-by":"crossref","first-page":"16","DOI":"10.1124\/pr.110.002790","article-title":"The blood-testis barrier and its implications for male contraception","volume":"64","author":"Cheng","year":"2012","journal-title":"Pharmacol Rev"},{"key":"2023063013481218000_gaad020-B8","doi-asserted-by":"crossref","first-page":"810","DOI":"10.1016\/j.fertnstert.2018.08.015","article-title":"Male infertility as a window to health","volume":"110","author":"Choy","year":"2018","journal-title":"Fertil Steril"},{"key":"2023063013481218000_gaad020-B9","doi-asserted-by":"crossref","first-page":"S72","DOI":"10.1006\/rtph.1997.1142","article-title":"Establishing an occupational exposure limit for hexavalent chromium in the European Union","volume":"26","author":"Cross","year":"1997","journal-title":"Regul Toxicol 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