{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,27]],"date-time":"2026-07-27T23:42:44Z","timestamp":1785195764822,"version":"3.55.0"},"reference-count":57,"publisher":"Oxford University Press (OUP)","issue":"3","license":[{"start":{"date-parts":[[2024,2,10]],"date-time":"2024-02-10T00:00:00Z","timestamp":1707523200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"name":"Eunice Kennedy Shriver NICHD"},{"DOI":"10.13039\/100000002","name":"NIH","doi-asserted-by":"publisher","award":["HD-038082"],"award-info":[{"award-number":["HD-038082"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"NIH","doi-asserted-by":"publisher","award":["HD088571"],"award-info":[{"award-number":["HD088571"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"NIH","doi-asserted-by":"publisher","award":["HD069631"],"award-info":[{"award-number":["HD069631"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"name":"National Institute of Health"},{"DOI":"10.13039\/100000066","name":"National Institute of Environmental Health Sciences","doi-asserted-by":"publisher","award":["R01ES030942"],"award-info":[{"award-number":["R01ES030942"]}],"id":[{"id":"10.13039\/100000066","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100015747","name":"University Grants Commission","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100015747","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Biotechnology Training Program"},{"name":"National Research Service Award","award":["T32 GM108556"],"award-info":[{"award-number":["T32 GM108556"]}]},{"name":"Consejo Nacional de Humanidades Ciencia y Tecnolog\u00eda de M\u00e9xico"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,2,29]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>To become fertile, mammalian sperm are required to undergo capacitation in the female tract or in vitro in defined media containing ions (e.g. HCO3\u2002\u2212, Ca2+, Na+, and Cl\u2212), energy sources (e.g. glucose, pyruvate) and serum albumin (e.g. bovine serum albumin (BSA)). These different molecules initiate sequential and concomitant signaling pathways, leading to capacitation. Physiologically, capacitation induces changes in the sperm motility pattern (e.g. hyperactivation) and prepares sperm for the acrosomal reaction (AR), two events required for fertilization. Molecularly, HCO3\u2002\u2212 activates the atypical adenylyl cyclase Adcy10 (aka sAC), increasing cAMP and downstream cAMP-dependent pathways. BSA, on the other hand, induces sperm cholesterol release as well as other signaling pathways. How these signaling events, occurring in different sperm compartments and with different kinetics, coordinate among themselves is not well established. Regarding the AR, recent work has proposed a role for glycogen synthase kinases (GSK3\u03b1 and GSK3\u03b2). GSK3\u03b1 and GSK3\u03b2 are inactivated by phosphorylation of residues Ser21 and Ser9, respectively, in their N-terminal domain. Here, we present evidence that GSK3\u03b1 (but not GSK3\u03b2) is present in the anterior head and that it is regulated during capacitation. Interestingly, BSA and HCO3\u2002\u2212 regulate GSK3\u03b1 in opposite directions. While BSA induces a fast GSK3\u03b1 Ser21 phosphorylation, HCO3\u2002\u2212 and cAMP-dependent pathways dephosphorylate this residue. We also show that the HCO3\u2212-induced Ser21 dephosphorylation is mediated by hyperpolarization of the sperm plasma membrane potential (Em) and by intracellular pH alkalinization. Previous reports indicate that GSK3 kinases mediate the progesterone-induced AR. Here, we show that GSK3 inhibition also blocks the Ca2+ ionophore ionomycin-induced AR, suggesting a role for GSK3 kinases downstream of the increase in intracellular Ca2+ needed for this exocytotic event. Altogether, our data indicate a temporal and biphasic GSK3\u03b1 regulation with opposite actions of BSA and HCO3\u2002\u2212. Our results also suggest that this regulation is needed to orchestrate the AR during sperm capacitation.<\/jats:p>","DOI":"10.1093\/molehr\/gaae007","type":"journal-article","created":{"date-parts":[[2024,2,9]],"date-time":"2024-02-09T08:07:18Z","timestamp":1707466038000},"source":"Crossref","is-referenced-by-count":10,"title":["Differential role of bovine serum albumin and HCO3\u2212 in the regulation of GSK3 alpha during mouse sperm capacitation"],"prefix":"10.1093","volume":"30","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0857-5509","authenticated-orcid":false,"given":"Gayatri","family":"Mohanty","sequence":"first","affiliation":[{"name":"Department of Veterinary and Animal Sciences, University of Massachusetts , Amherst, MA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claudia","family":"Sanchez-Cardenas","sequence":"additional","affiliation":[{"name":"Departamento de Gen\u00e9tica del Desarrollo y Fisiolog\u00eda Molecular, Instituto de Biotecnolog\u00eda, UNAM , Cuernavaca, M\u00e9xico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bidur","family":"Paudel","sequence":"additional","affiliation":[{"name":"Department of Veterinary and Animal Sciences, University of Massachusetts , Amherst, MA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Darya A","family":"Tourzani","sequence":"additional","affiliation":[{"name":"Department of Veterinary and Animal Sciences, University of Massachusetts , Amherst, MA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana M","family":"Salicioni","sequence":"additional","affiliation":[{"name":"Department of Veterinary and Animal Sciences, University of Massachusetts , Amherst, MA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Celia M","family":"Santi","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, Washington University School of Medicine , St Louis, MO, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mar\u00eda G","family":"Gervasi","sequence":"additional","affiliation":[{"name":"Department of Veterinary and Animal Sciences, University of Massachusetts , Amherst, MA, USA"},{"name":"Department of Animal Science, University of Connecticut , Storrs, CT, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4541-6267","authenticated-orcid":false,"given":"J Richard","family":"Pilsner","sequence":"additional","affiliation":[{"name":"C.S. Mott Center for Human Growth and Development, Department of Obstetrics and Gynecology, School of Medicine, Wayne State University, Detroit, MI, USA"},{"name":"Institute of Environmental Health Sciences, Wayne State University , Detroit, MI, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2502-0505","authenticated-orcid":false,"given":"Alberto","family":"Darszon","sequence":"additional","affiliation":[{"name":"Departamento de Gen\u00e9tica del Desarrollo y Fisiolog\u00eda Molecular, Instituto de Biotecnolog\u00eda, UNAM , Cuernavaca, M\u00e9xico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9320-7518","authenticated-orcid":false,"given":"Pablo E","family":"Visconti","sequence":"additional","affiliation":[{"name":"Department of Veterinary and Animal Sciences, University of Massachusetts , Amherst, MA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2024,2,10]]},"reference":[{"key":"2024030523381888900_gaae007-B1","doi-asserted-by":"crossref","first-page":"gaab054","DOI":"10.1093\/molehr\/gaab054","article-title":"Soluble adenylyl cyclase inhibition prevents human sperm functions essential for fertilization","volume":"27","author":"Balbach","year":"2021","journal-title":"Mol Hum Reprod"},{"key":"2024030523381888900_gaae007-B2","doi-asserted-by":"crossref","first-page":"637","DOI":"10.1038\/s41467-023-36119-6","article-title":"On-demand male contraception via acute inhibition of soluble adenylyl cyclase","volume":"14","author":"Balbach","year":"2023","journal-title":"Nat Commun"},{"key":"2024030523381888900_gaae007-B3","doi-asserted-by":"crossref","first-page":"e21478","DOI":"10.1096\/fj.202002333RR","article-title":"Membrane hyperpolarization abolishes calcium oscillations that prevent induced acrosomal exocytosis in human sperm","volume":"35","author":"Balestrini","year":"2021","journal-title":"FASEB J"},{"key":"2024030523381888900_gaae007-B4","doi-asserted-by":"crossref","first-page":"384","DOI":"10.1093\/biolre\/ioy020","article-title":"Isoform-specific requirement for GSK3\u03b1 in sperm for male fertility","volume":"99","author":"Bhattacharjee","year":"2018","journal-title":"Biol Reprod"},{"key":"2024030523381888900_gaae007-B5","doi-asserted-by":"crossref","first-page":"65","DOI":"10.1095\/biolreprod.114.124495","article-title":"Targeted disruption of glycogen synthase kinase 3A (GSK3A) in mice affects sperm motility resulting in male infertility","volume":"92","author":"Bhattacharjee","year":"2015","journal-title":"Biol Reprod"},{"key":"2024030523381888900_gaae007-B6","doi-asserted-by":"crossref","first-page":"8656","DOI":"10.1074\/jbc.M508854200","article-title":"Calcium-induced acrosomal exocytosis requires cAMP acting through a protein kinase A-independent, Epac-mediated pathway","volume":"281","author":"Branham","year":"2006","journal-title":"J Biol Chem"},{"key":"2024030523381888900_gaae007-B7","doi-asserted-by":"crossref","first-page":"2610","DOI":"10.1016\/j.bbadis.2014.07.013","article-title":"Central role of soluble adenylyl cyclase and cAMP in sperm physiology","volume":"1842","author":"Buffone","year":"2014","journal-title":"Biochim Biophys Acta"},{"key":"2024030523381888900_gaae007-B8","doi-asserted-by":"crossref","first-page":"e60578","DOI":"10.1371\/journal.pone.0060578","article-title":"Ion permeabilities in mouse sperm reveal an external trigger for SLO3-dependent hyperpolarization","volume":"8","author":"Ch\u00e1vez","year":"2013","journal-title":"PLoS One"},{"key":"2024030523381888900_gaae007-B9","doi-asserted-by":"crossref","first-page":"551","DOI":"10.3390\/s16040551","article-title":"Rapid detection of glycogen synthase kinase-3 activity in mouse sperm using fluorescent gel shift electrophoresis","volume":"16","author":"Choi","year":"2016","journal-title":"Sensors (Basel)"},{"key":"2024030523381888900_gaae007-B10","doi-asserted-by":"crossref","first-page":"1328","DOI":"10.1095\/biolreprod59.6.1328","article-title":"Cyclodextrin removes cholesterol from mouse sperm and induces capacitation in a protein-free medium","volume":"59","author":"Choi","year":"1998","journal-title":"Biol Reprod"},{"key":"2024030523381888900_gaae007-B11","doi-asserted-by":"crossref","first-page":"44384","DOI":"10.1074\/jbc.M112.393488","article-title":"Mouse sperm membrane potential hyperpolarization is necessary and sufficient to prepare sperm for the acrosome reaction","volume":"287","author":"De La Vega-Beltran","year":"2012","journal-title":"J Biol Chem"},{"key":"2024030523381888900_gaae007-B12","doi-asserted-by":"crossref","first-page":"7001","DOI":"10.1074\/jbc.M206284200","article-title":"Involvement of a Na+\/HCO3\u2212 cotransporter in mouse sperm capacitation","volume":"278","author":"Demarco","year":"2003","journal-title":"J Biol Chem"},{"key":"2024030523381888900_gaae007-B13","doi-asserted-by":"crossref","first-page":"1247","DOI":"10.1096\/fj.201902163R","article-title":"Roles of glycogen synthase kinase 3 alpha and calcineurin in regulating the ability of sperm to fertilize eggs","volume":"34","author":"Dey","year":"2020","journal-title":"Faseb J"},{"key":"2024030523381888900_gaae007-B14","doi-asserted-by":"crossref","first-page":"5329","DOI":"10.3390\/ijms24065329","article-title":"The SLC9C2 gene product (Na+\/H+ exchanger isoform 11; NHE11) is a testis-specific protein localized to the head of mature mammalian sperm","volume":"24","author":"Gardner","year":"2023","journal-title":"Int J Mol Sci"},{"key":"2024030523381888900_gaae007-B15","doi-asserted-by":"crossref","first-page":"860","DOI":"10.1002\/mrd.22663","article-title":"Chang\u2019s meaning of capacitation: A molecular perspective","volume":"83","author":"Gervasi","year":"2016","journal-title":"Mol Reprod Dev"},{"key":"2024030523381888900_gaae007-B16","doi-asserted-by":"crossref","first-page":"204","DOI":"10.1111\/andr.12320","article-title":"Molecular changes and signaling events occurring in spermatozoa during epididymal maturation","volume":"5","author":"Gervasi","year":"2017","journal-title":"Andrology"},{"key":"2024030523381888900_gaae007-B17","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1095\/biolreprod32.1.145","article-title":"Albumin-mediated changes in sperm sterol content during capacitation","volume":"32","author":"Go","year":"1985","journal-title":"Biol Reprod"},{"key":"2024030523381888900_gaae007-B18","doi-asserted-by":"crossref","first-page":"125","DOI":"10.1530\/REP-22-0101","article-title":"Hyperpolarization induces cytosolic alkalization of mouse sperm flagellum probably through sperm Na+\/H+ exchanger","volume":"164","author":"Hern\u00e1ndez-Gardu\u00f1o","year":"2022","journal-title":"Reproduction"},{"key":"2024030523381888900_gaae007-B19","doi-asserted-by":"crossref","first-page":"86","DOI":"10.1038\/35017574","article-title":"Requirement for glycogen synthase kinase-3beta in cell survival and NF-kappaB activation","volume":"406","author":"Hoeflich","year":"2000","journal-title":"Nature"},{"key":"2024030523381888900_gaae007-B20","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1186\/1756-6606-2-35","article-title":"Abnormalities in brain structure and behavior in GSK-3alpha mutant mice","volume":"2","author":"Kaidanovich-Beilin","year":"2009","journal-title":"Mol Brain"},{"key":"2024030523381888900_gaae007-B21","doi-asserted-by":"crossref","first-page":"jcs238816","DOI":"10.1242\/jcs.238816","article-title":"Capacitation-associated alkalization in human sperm is differentially controlled at the subcellular level","volume":"133","author":"Matamoros-Volante","year":"2020","journal-title":"J Cell Sci"},{"key":"2024030523381888900_gaae007-B22","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1186\/1756-6606-6-27","article-title":"Neurological characterization of mice deficient in GSK3\u03b1 highlight pleiotropic physiological functions in cognition and pathological activity as Tau kinase","volume":"6","author":"Maurin","year":"2013","journal-title":"Mol Brain"},{"key":"2024030523381888900_gaae007-B23","doi-asserted-by":"crossref","first-page":"1484","DOI":"10.1111\/andr.13423","article-title":"Effects of preconception exposure to phthalates on mouse sperm capacitation parameters","volume":"11","author":"Mohanty","year":"2023","journal-title":"Andrology"},{"key":"2024030523381888900_gaae007-B24","doi-asserted-by":"crossref","first-page":"607","DOI":"10.1387\/ijdb.072554bn","article-title":"Ion channels that control fertility in mammalian spermatozoa","volume":"52","author":"Navarro","year":"2008","journal-title":"Int J Dev Biol"},{"key":"2024030523381888900_gaae007-B25","doi-asserted-by":"crossref","first-page":"718","DOI":"10.1002\/mrd.23539","article-title":"TSSK3, a novel target for male contraception, is required for spermiogenesis","volume":"88","author":"Nayyab","year":"2021","journal-title":"Mol Reprod Dev"},{"key":"2024030523381888900_gaae007-B26","doi-asserted-by":"crossref","first-page":"1149","DOI":"10.1016\/j.bbrc.2014.05.100","article-title":"Intracellular pH in sperm physiology","volume":"450","author":"Nishigaki","year":"2014","journal-title":"Biochem Biophys Res Commun"},{"key":"2024030523381888900_gaae007-B27","doi-asserted-by":"crossref","first-page":"1017","DOI":"10.1093\/molehr\/5.11.1017","article-title":"Regulation of human sperm capacitation by a cholesterol efflux-stimulated signal transduction pathway leading to protein kinase A-mediated up-regulation of protein tyrosine phosphorylation","volume":"5","author":"Osheroff","year":"1999","journal-title":"Mol Hum Reprod"},{"key":"2024030523381888900_gaae007-B28","doi-asserted-by":"crossref","first-page":"215","DOI":"10.5534\/wjmh.220108","article-title":"Glycogen synthase kinase-3 isoform variants and their inhibitory phosphorylation in human testes and spermatozoa","volume":"41","author":"Park","year":"2023","journal-title":"World J Mens Health"},{"key":"2024030523381888900_gaae007-B29","doi-asserted-by":"crossref","first-page":"885","DOI":"10.1111\/j.1476-5381.2008.00085.x","article-title":"Glycogen synthase kinase 3: more than a namesake","volume":"156","author":"Rayasam","year":"2009","journal-title":"Br J Pharmacol"},{"key":"2024030523381888900_gaae007-B30","doi-asserted-by":"crossref","first-page":"2872","DOI":"10.1096\/fj.14-265553","article-title":"Glycogen synthase kinase 3 regulates acrosomal exocytosis in mouse spermatozoa via dynamin phosphorylation","volume":"29","author":"Reid","year":"2015","journal-title":"Faseb J"},{"key":"2024030523381888900_gaae007-B31","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1016\/j.mod.2018.04.004","article-title":"Regulation mechanisms and implications of sperm membrane hyperpolarization","volume":"154","author":"Ritagliati","year":"2018","journal-title":"Mech Dev"},{"key":"2024030523381888900_gaae007-B32","doi-asserted-by":"crossref","first-page":"264","DOI":"10.1093\/biolre\/ioaa064","article-title":"Testis-specific serine kinase protein family in male fertility and as targets for non-hormonal male contraception","volume":"103","author":"Salicioni","year":"2020","journal-title":"Biol Reprod"},{"key":"2024030523381888900_gaae007-B33","doi-asserted-by":"crossref","first-page":"e21528","DOI":"10.1096\/fj.202100122R","article-title":"Starvation induces an increase in intracellular calcium and potentiates the progesterone-induced mouse sperm acrosome reaction","volume":"35","author":"S\u00e1nchez-C\u00e1rdenas","year":"2021","journal-title":"Faseb J."},{"key":"2024030523381888900_gaae007-B34","doi-asserted-by":"crossref","first-page":"1041","DOI":"10.1016\/j.febslet.2010.02.005","article-title":"The SLO3 sperm-specific potassium channel plays a vital role in male fertility","volume":"584","author":"Santi","year":"2010","journal-title":"FEBS Lett"},{"key":"2024030523381888900_gaae007-B35","doi-asserted-by":"crossref","first-page":"3509","DOI":"10.1074\/jbc.273.6.3509","article-title":"SLO3, a novel pH-sensitive K+ channel from mammalian spermatocytes","volume":"273","author":"Schreiber","year":"1998","journal-title":"J Biol Chem"},{"key":"2024030523381888900_gaae007-B36","doi-asserted-by":"crossref","first-page":"331","DOI":"10.1242\/jcs.059949","article-title":"Functional transformation of the chromatoid body in mouse spermatids requires testis-specific serine\/threonine kinases","volume":"123","author":"Shang","year":"2010","journal-title":"J Cell Sci"},{"key":"2024030523381888900_gaae007-B37","first-page":"630","article-title":"Mutation of the Calpha subunit of PKA leads to growth retardation and sperm dysfunction","volume":"16","author":"Sk\u00e5lhegg","year":"2002","journal-title":"Mol Endocrinol"},{"key":"2024030523381888900_gaae007-B38","doi-asserted-by":"crossref","first-page":"4250","DOI":"10.1128\/MCB.25.10.4250-4261.2005","article-title":"Identification and characterization of SSTK, a serine\/threonine protein kinase essential for male fertility","volume":"25","author":"Spiridonov","year":"2005","journal-title":"Mol Cell Biol"},{"key":"2024030523381888900_gaae007-B39","doi-asserted-by":"crossref","first-page":"16935","DOI":"10.1021\/acs.langmuir.9b03064","article-title":"How valinomycin ionophores enter and transport K+ across model lipid bilayer membranes","volume":"35","author":"Su","year":"2019","journal-title":"Langmuir"},{"key":"2024030523381888900_gaae007-B40","doi-asserted-by":"crossref","first-page":"544","DOI":"10.1002\/mrd.23524","article-title":"Activation of cAMP-dependent phosphorylation pathways is independent of ROS production during mouse sperm capacitation","volume":"88","author":"Takei","year":"2021","journal-title":"Mol Reprod Dev"},{"key":"2024030523381888900_gaae007-B41","doi-asserted-by":"crossref","first-page":"227","DOI":"10.1016\/S0889-1605(86)80022-2","article-title":"Distribution of sterols and anionic lipids in human sperm plasma membrane: Effects of in vitro capacitation","volume":"97","author":"Tesa\u0159\u00edk","year":"1986","journal-title":"J. Ultrastruct Res"},{"key":"2024030523381888900_gaae007-B42","doi-asserted-by":"crossref","first-page":"10241","DOI":"10.3390\/ijms221910241","article-title":"Caput ligation renders immature mouse sperm motile and capable to undergo cAMP-dependent phosphorylation","volume":"22","author":"Tourzani","year":"2021","journal-title":"Int J Mol Sci"},{"key":"2024030523381888900_gaae007-B43","doi-asserted-by":"crossref","first-page":"60","DOI":"10.3389\/fcell.2018.00060","article-title":"Changes in protein O-GlcNacylation during mouse epididymal sperm maturation","volume":"6","author":"Tourzani","year":"2018","journal-title":"Front Cell Dev Biol"},{"key":"2024030523381888900_gaae007-B44","doi-asserted-by":"crossref","first-page":"4350","DOI":"10.1073\/pnas.76.9.4350","article-title":"Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications","volume":"76","author":"Towbin","year":"1979","journal-title":"Proc Natl Acad Sci USA"},{"key":"2024030523381888900_gaae007-B45","doi-asserted-by":"crossref","first-page":"731","DOI":"10.1172\/JCI0216392","article-title":"The role of cholesterol efflux in regulating the fertilization potential of mammalian spermatozoa","volume":"110","author":"Travis","year":"2002","journal-title":"J Clin Invest"},{"key":"2024030523381888900_gaae007-B46","doi-asserted-by":"crossref","first-page":"667","DOI":"10.1073\/pnas.0811895106","article-title":"Understanding the molecular basis of sperm capacitation through kinase design","volume":"106","author":"Visconti","year":"2009","journal-title":"Proc Natl Acad Sci USA"},{"key":"2024030523381888900_gaae007-B47","doi-asserted-by":"crossref","first-page":"1129","DOI":"10.1242\/dev.121.4.1129","article-title":"Capacitation of mouse spermatozoa. I. Correlation between the capacitation state and protein tyrosine phosphorylation","volume":"121","author":"Visconti","year":"1995","journal-title":"Development"},{"key":"2024030523381888900_gaae007-B48","doi-asserted-by":"crossref","first-page":"3235","DOI":"10.1074\/jbc.274.5.3235","article-title":"Cholesterol efflux-mediated signal transduction in mammalian sperm. beta-cyclodextrins initiate transmembrane signaling leading to an increase in protein tyrosine phosphorylation and capacitation","volume":"274","author":"Visconti","year":"1999","journal-title":"J Biol Chem"},{"key":"2024030523381888900_gaae007-B49","doi-asserted-by":"crossref","first-page":"429","DOI":"10.1006\/dbio.1999.9428","article-title":"Cholesterol efflux-mediated signal transduction in mammalian sperm: cholesterol release signals an increase in protein tyrosine phosphorylation during mouse sperm capacitation","volume":"214","author":"Visconti","year":"1999","journal-title":"Dev Biol"},{"key":"2024030523381888900_gaae007-B50","doi-asserted-by":"crossref","first-page":"82","DOI":"10.1002\/(SICI)1098-2795(199601)43:1<82::AID-MRD11>3.0.CO;2-6","article-title":"Properties and localization of a tyrosine phosphorylated form of hexokinase in mouse sperm","volume":"43","author":"Visconti","year":"1996","journal-title":"Mol Reprod Dev"},{"key":"2024030523381888900_gaae007-B51","doi-asserted-by":"crossref","first-page":"9325","DOI":"10.1073\/pnas.0611296104","article-title":"A sperm-specific Na+\/H+ exchanger (sNHE) is critical for expression and in vivo bicarbonate regulation of the soluble adenylyl cyclase (sAC)","volume":"104","author":"Wang","year":"2007","journal-title":"Proc Natl Acad Sci USA"},{"key":"2024030523381888900_gaae007-B52","doi-asserted-by":"crossref","first-page":"35307","DOI":"10.1074\/jbc.M113.489476","article-title":"Compartmentalization of distinct cAMP signaling pathways in mammalian sperm","volume":"288","author":"Wertheimer","year":"2013","journal-title":"J Biol Chem"},{"key":"2024030523381888900_gaae007-B53","doi-asserted-by":"crossref","first-page":"4210","DOI":"10.1038\/s41467-020-18023-5","article-title":"Author correction: the solute carrier SLC9C1 is a Na+\/H+-exchanger gated by an S4-type voltage-sensor and cyclic-nucleotide binding","volume":"11","author":"Windler","year":"2020","journal-title":"Nat Commun"},{"key":"2024030523381888900_gaae007-B54","doi-asserted-by":"crossref","first-page":"448","DOI":"10.1038\/78153","article-title":"Spermiogenesis and exchange of basic nuclear proteins are impaired in male germ cells lacking Camk4","volume":"25","author":"Wu","year":"2000","journal-title":"Nat Genet"},{"key":"2024030523381888900_gaae007-B55","doi-asserted-by":"crossref","first-page":"119","DOI":"10.1186\/1477-7827-7-119","article-title":"The BSA-induced Ca2+ influx during sperm capacitation is CATSPER channel-dependent","volume":"7","author":"Xia","year":"2009","journal-title":"Reprod Biol Endocrinol"},{"key":"2024030523381888900_gaae007-B56","doi-asserted-by":"crossref","first-page":"1092","DOI":"10.1095\/biolreprod.108.074039","article-title":"Egg coat proteins activate calcium entry into mouse sperm via CATSPer channels","volume":"80","author":"Xia","year":"2009","journal-title":"Biol Reprod"},{"key":"2024030523381888900_gaae007-B57","doi-asserted-by":"crossref","first-page":"118","DOI":"10.1038\/12729","article-title":"Globozoospermia in mice lacking the casein kinase II alpha\u2019 catalytic subunit","volume":"23","author":"Xu","year":"1999","journal-title":"Nat Genet"}],"container-title":["Molecular Human Reproduction"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/molehr\/advance-article-pdf\/doi\/10.1093\/molehr\/gaae007\/56661078\/gaae007.pdf","content-type":"application\/pdf","content-version":"am","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/molehr\/article-pdf\/30\/3\/gaae007\/56851003\/gaae007.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/molehr\/article-pdf\/30\/3\/gaae007\/56851003\/gaae007.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,3,5]],"date-time":"2024-03-05T23:39:29Z","timestamp":1709681969000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/molehr\/article\/doi\/10.1093\/molehr\/gaae007\/7606333"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,2,10]]},"references-count":57,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2024,2,29]]}},"URL":"https:\/\/doi.org\/10.1093\/molehr\/gaae007","relation":{},"ISSN":["1460-2407"],"issn-type":[{"value":"1460-2407","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2024,3,1]]},"published":{"date-parts":[[2024,2,10]]},"article-number":"gaae007"}}