{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T16:20:35Z","timestamp":1791908435992,"version":"4.3.4"},"reference-count":47,"publisher":"Oxford University Press (OUP)","issue":"5","license":[{"start":{"date-parts":[[2024,4,30]],"date-time":"2024-04-30T00:00:00Z","timestamp":1714435200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"name":"Faculty of Health Sciences of Southern Denmark University"},{"DOI":"10.13039\/501100024075","name":"Region of Southern Denmark","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100024075","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,4,30]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Studies in humans and animals suggest that seminal plasma, the acellular seminal fluid component, stimulates the endometrium to promote immune tolerance and facilitate implantation. We designed a randomized, double-blinded, placebo-controlled trial to investigate changes in the endometrial transcriptomic profile after vaginal application of seminal plasma. The study participants were randomized into two groups. Five women received a vaginal application of seminal plasma, and four received a placebo application with saline solution. The application was performed 2 days after HCG-triggered ovulation in an unstimulated cycle. After 5\u20138\u2009days, an endometrial biopsy was collected to analyze differences in the endometrial transcriptomic profile using microarray analyses. A differential gene expression analysis and a gene set analysis were performed. The gene set enrichment analysis showed a positive enrichment of pathways associated with the immune response, cell viability, proliferation, and cellular movement. Moreover, pathways involved in implantation, embryo development, oocyte maturation, and angiogenesis were positively enriched. The differential gene expression analysis, after adjusting for multiple testing, showed no significantly differentially expressed genes between the two groups. A comparative analysis was also performed with similar studies conducted in other animals or in vitro using human endometrial cells. The comparative analysis showed that the effect of seminal plasma effect on the endometrium is similar in pigs, mice, and in vitro human endometrial cells. The present study provides evidence that seminal plasma might impact the endometrium during the implantation window, with potential to affect endometrial receptivity and embryo development.<\/jats:p>","DOI":"10.1093\/molehr\/gaae017","type":"journal-article","created":{"date-parts":[[2024,5,11]],"date-time":"2024-05-11T14:24:34Z","timestamp":1715437474000},"source":"Crossref","is-referenced-by-count":13,"title":["<i>In vivo<\/i>\n                    effect of vaginal seminal plasma application on the human endometrial transcriptome: a randomized controlled trial"],"prefix":"10.1093","volume":"30","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4194-4281","authenticated-orcid":false,"given":"Laura","family":"Catalini","sequence":"first","affiliation":[{"name":"Research Unit of Gynecology and Obstetrics, Department of Clinical Research, University of Southern Denmark , Odense, Denmark"},{"name":"Centre of Andrology and Fertility Clinic, Department of Gynecology and Obstetrics, Odense University Hospital , Odense, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mark","family":"Burton","sequence":"additional","affiliation":[{"name":"Research Unit of Clinical Genetics, Department of Clinical Research, Faculty of Health Sciences, University of Southern Denmark , Odense, Denmark"},{"name":"Department of Clinical Genetics, Odense University Hospital , Odense, Denmark"},{"name":"Clinical Genome Center, University of Southern Denmark & Region of Southern Denmark , Odense, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dorte L","family":"Egeberg","sequence":"additional","affiliation":[{"name":"European Sperm Bank , Copenhagen, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tilde V","family":"Eskildsen","sequence":"additional","affiliation":[{"name":"Research Unit of Gynecology and Obstetrics, Department of Clinical Research, University of Southern Denmark , Odense, Denmark"},{"name":"Centre of 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