{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T09:20:52Z","timestamp":1785403252399,"version":"3.56.0"},"reference-count":49,"publisher":"Oxford University Press (OUP)","issue":"7","license":[{"start":{"date-parts":[[2024,6,13]],"date-time":"2024-06-13T00:00:00Z","timestamp":1718236800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"name":"Hunan Provincial Key Laboratory of Regional Hereditary Birth Defects Prevention and Control"},{"name":"Clinical Medical Technology Innovation Guidance Project of Hunan Province","award":["2021SK53716"],"award-info":[{"award-number":["2021SK53716"]}]},{"name":"Hunan Province Assisted Reproduction and Regenerative Medicine Clinical Demonstration Center Funded","award":["2020SK4019"],"award-info":[{"award-number":["2020SK4019"]}]},{"name":"Undergraduate and Graduate Student Independent Exploration Innovation Fund of the Central South University","award":["1053320215869"],"award-info":[{"award-number":["1053320215869"]}]},{"name":"Undergraduate and Graduate Student Independent Exploration Innovation Fund of the Central South University","award":["1053320214303"],"award-info":[{"award-number":["1053320214303"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,6,26]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Acephalic spermatozoa syndrome (ASS) is a severe teratospermia with decaudated, decapitated, and malformed sperm, resulting in male infertility. Nuclear envelope protein SUN5 localizes to the junction between the sperm head and tail. Mutations in the SUN5 gene have been identified most frequently (33\u201347%) in ASS cases, and its molecular mechanism of action is yet to be explored. In the present study, we generated Sun5 knockout mice, which presented the phenotype of ASS. Nuclear membrane protein LaminB1 and cytoskeletal GTPases Septin12 and Septin2 were identified as potential partners for interacting with SUN5 by immunoprecipitation\u2013mass spectrometry in mouse testis. Further studies demonstrated that SUN5 connected the nucleus by interacting with LaminB1 and connected the proximal centriole by interacting with Septin12. The binding between SUN5 and Septin12 promoted their aggregation together in the sperm neck. The disruption of the LaminB1\/SUN5\/Septin12 complex by Sun5 deficiency caused separation of the Septin12-proximal centriole from the nucleus, leading to the breakage of the head-to-tail junction. Collectively, these data provide new insights into the pathogenesis of ASS caused by SUN5 deficiency.<\/jats:p>","DOI":"10.1093\/molehr\/gaae022","type":"journal-article","created":{"date-parts":[[2024,6,14]],"date-time":"2024-06-14T11:51:56Z","timestamp":1718365916000},"source":"Crossref","is-referenced-by-count":15,"title":["SUN5 interacts with nuclear membrane LaminB1 and cytoskeletal GTPase Septin12 mediating the sperm head-and-tail junction"],"prefix":"10.1093","volume":"30","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0483-2253","authenticated-orcid":false,"given":"Yunfei","family":"Zhang","sequence":"first","affiliation":[{"name":"Department of Laboratory Medicine, The Third Xiangya Hospital, Central South University , Changsha, China"},{"name":"Center for Experimental Medicine, The Third Xiangya Hospital, Central South University , Changsha, 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