{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,6]],"date-time":"2026-06-06T09:01:10Z","timestamp":1780736470415,"version":"3.54.1"},"reference-count":30,"publisher":"Oxford University Press (OUP)","issue":"2","license":[{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"name":"the Sichuan Provincial Health and Wellness Committee","award":["20PJ085"],"award-info":[{"award-number":["20PJ085"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,4,11]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>The testis-expressed (TEX\/Tex) genes are highly expressed in the testes of various species. Several members of the TEX catalog have been suggested to play a role in spermatogenesis, but the functions of most members remain unknown. Here, we initially confirmed that TEX2 is significantly upregulated in spermatids and further successfully generated a Tex2 knockout (KO) mouse model using CRISPR\/Cas9 technology. However, we found that the Tex2 KO male mice were fertile. Interestingly, Papanicolaou staining and scanning electron microscopy showed slight abnormalities in sperm morphology and reduced sperm motility as assessed by computer-assisted sperm analysis in Tex2 KO mice. Collectively, despite Tex2 having enriched expression in mouse testis, our genetic KO studies revealed that Tex2 is not essential for spermatogenesis or fertility in male mice, although its absence in sperm causes subtle abnormalities in morphology and motility.<\/jats:p>","DOI":"10.1093\/molehr\/gaag031","type":"journal-article","created":{"date-parts":[[2026,5,22]],"date-time":"2026-05-22T11:46:56Z","timestamp":1779450416000},"source":"Crossref","is-referenced-by-count":0,"title":["Investigating the role of a testis-expressed gene\n                    <i>Tex2<\/i>\n                    in spermatogenesis in mice"],"prefix":"10.1093","volume":"32","author":[{"given":"Guicheng","family":"Zhao","sequence":"first","affiliation":[{"name":"Reproductive Andrology and Sichuan Human Sperm Bank, Key Laboratory of Obstetric, Gynecologic and Pediatric Diseases and Birth Defects of Ministry of Education, West China Second University Hospital, Sichuan University , Chengdu, Sichuan,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Suyan","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Obstetrics\/Gynecology, Key Laboratory of Obstetric, Gynecologic and Pediatric Diseases and Birth Defects of Ministry of Education, West China Second University Hospital, Sichuan University , Chengdu, Sichuan,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gan","family":"Shen","sequence":"additional","affiliation":[{"name":"Department of Obstetrics\/Gynecology, Key Laboratory of Obstetric, Gynecologic and Pediatric Diseases and Birth Defects of Ministry of Education, West China Second University Hospital, Sichuan University , Chengdu, Sichuan,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liangchai","family":"Zhuo","sequence":"additional","affiliation":[{"name":"Department of Obstetrics\/Gynecology, Key Laboratory of Obstetric, Gynecologic and Pediatric Diseases and Birth Defects of Ministry of Education, West China Second University Hospital, Sichuan University , Chengdu, Sichuan,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tiechao","family":"Ruan","sequence":"additional","affiliation":[{"name":"Department of Obstetrics\/Gynecology, Key Laboratory of Obstetric, Gynecologic and Pediatric Diseases and Birth Defects of Ministry of Education, West China Second University Hospital, Sichuan University , Chengdu, Sichuan,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiang","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Obstetrics\/Gynecology, Key Laboratory of Obstetric, Gynecologic and Pediatric Diseases and Birth Defects of Ministry of Education, West China Second University Hospital, Sichuan University , Chengdu, Sichuan,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chuan","family":"Jiang","sequence":"additional","affiliation":[{"name":"Department of Obstetrics\/Gynecology, Key Laboratory of Obstetric, Gynecologic and Pediatric Diseases and Birth Defects of Ministry of Education, West China Second University Hospital, Sichuan University , Chengdu, Sichuan,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yao","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Obstetrics\/Gynecology, Key Laboratory of Obstetric, Gynecologic and Pediatric Diseases and Birth Defects of Ministry of Education, West China Second University Hospital, Sichuan University , Chengdu, Sichuan,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5165-1442","authenticated-orcid":false,"given":"Xiaohui","family":"Jiang","sequence":"additional","affiliation":[{"name":"Reproductive Andrology and Sichuan Human Sperm Bank, Key Laboratory of Obstetric, Gynecologic and Pediatric Diseases and Birth Defects of Ministry of Education, West China Second University Hospital, Sichuan University , Chengdu, Sichuan,","place":["China"]},{"name":"Tianfu Jincheng Laboratory, City of Future Medicine , Chengdu, Sichuan,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-5905-6195","authenticated-orcid":false,"given":"Dingming","family":"Li","sequence":"additional","affiliation":[{"name":"Reproductive Andrology and Sichuan Human Sperm Bank, Key Laboratory of Obstetric, Gynecologic and Pediatric Diseases and Birth Defects of Ministry of Education, West China Second University Hospital, Sichuan University , Chengdu, Sichuan,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1484-0807","authenticated-orcid":false,"given":"Ying","family":"Shen","sequence":"additional","affiliation":[{"name":"Department of Obstetrics\/Gynecology, Key Laboratory of Obstetric, Gynecologic and Pediatric Diseases and Birth Defects of Ministry of Education, West China Second University Hospital, Sichuan University , Chengdu, Sichuan,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2026,5,25]]},"reference":[{"key":"2026060604203623800_gaag031-B1","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1002\/rmb2.12067","article-title":"Revolutionizing male fertility factor research in mice by using the genome editing tool CRISPR\/Cas9","volume":"17","author":"Abbasi","year":"2018","journal-title":"Reprod Med Biol"},{"key":"2026060604203623800_gaag031-B2","doi-asserted-by":"crossref","first-page":"e0175787","DOI":"10.1371\/journal.pone.0175787","article-title":"Comparative expression profiling of testis-enriched genes regulated during the development of spermatogonial cells","volume":"12","author":"Ahn","year":"2017","journal-title":"PLoS One"},{"key":"2026060604203623800_gaag031-B3","doi-asserted-by":"crossref","first-page":"1711","DOI":"10.3390\/cells11101711","article-title":"Infertility in men: advances towards a comprehensive and integrative strategy for precision theranostics","volume":"11","author":"Assidi","year":"2022","journal-title":"Cells"},{"key":"2026060604203623800_gaag031-B4","doi-asserted-by":"crossref","first-page":"29","DOI":"10.2147\/IJGM.S241099","article-title":"Some of the factors involved in male infertility: a prospective review","volume":"13","author":"Babakhanzadeh","year":"2020","journal-title":"Int J Gen Med"},{"key":"2026060604203623800_gaag031-B5","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1186\/s12610-021-00127-7","article-title":"Human testis-expressed (TEX) genes: a review focused on spermatogenesis and male fertility","volume":"31","author":"Bellil","year":"2021","journal-title":"Basic Clin Androl"},{"key":"2026060604203623800_gaag031-B6","first-page":"185","article-title":"Expression analysis of genes encoding TEX11, TEX12, TEX14 and TEX15 in testis tissues of men with non-obstructive azoospermia","volume":"22","author":"Boroujeni","year":"2018","journal-title":"JBRA Assist Reprod"},{"key":"2026060604203623800_gaag031-B7","first-page":"50648","article-title":"Separation of spermatogenic cell types using STA\u2013PUT velocity sedimentation","volume":"80","author":"Bryant","year":"2013","journal-title":"J Vis Exp"},{"key":"2026060604203623800_gaag031-B8","doi-asserted-by":"crossref","first-page":"892043","DOI":"10.3389\/fonc.2022.892043","article-title":"A role of the TEX101 interactome in the common aetiology behind male subfertility and testicular germ cell tumor","volume":"12","author":"Burton","year":"2022","journal-title":"Front Oncol"},{"key":"2026060604203623800_gaag031-B9","doi-asserted-by":"crossref","first-page":"e2206852","DOI":"10.1002\/advs.202206852","article-title":"Deciphering the molecular characteristics of human idiopathic nonobstructive azoospermia from the perspective of germ cells","volume":"10","author":"Chen","year":"2023","journal-title":"Adv Sci (Weinh)"},{"key":"2026060604203623800_gaag031-B10","doi-asserted-by":"crossref","first-page":"7287","DOI":"10.1007\/s11033-022-07512-z","article-title":"Fbxw17 is dispensable for viability and fertility in mice","volume":"49","author":"Chen","year":"2022","journal-title":"Mol Biol Rep"},{"key":"2026060604203623800_gaag031-B11","doi-asserted-by":"crossref","first-page":"810","DOI":"10.1016\/j.fertnstert.2018.08.015","article-title":"Male infertility as a window to health","volume":"110","author":"Choy","year":"2018","journal-title":"Fertil Steril"},{"key":"2026060604203623800_gaag031-B12","doi-asserted-by":"crossref","first-page":"615","DOI":"10.1007\/s10815-015-0433-2","article-title":"Genetic aspects of monomorphic teratozoospermia: a review","volume":"32","author":"De Braekeleer","year":"2015","journal-title":"J Assist Reprod Genet"},{"key":"2026060604203623800_gaag031-B13","doi-asserted-by":"crossref","first-page":"e202205133","DOI":"10.1083\/jcb.202205133","article-title":"Tex2 is required for lysosomal functions at TMEM55-dependent ER membrane contact sites","volume":"222","author":"Du","year":"2023","journal-title":"J Cell Biol"},{"key":"2026060604203623800_gaag031-B14","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1038\/s41572-023-00459-w","article-title":"Male infertility","volume":"9","author":"Eisenberg","year":"2023","journal-title":"Nat Rev Dis Primers"},{"key":"2026060604203623800_gaag031-B15","doi-asserted-by":"crossref","first-page":"6065","DOI":"10.1038\/s41467-021-26177-z","article-title":"PDZD-8 and TEX-2 regulate endosomal PI (4, 5) P2 homeostasis via lipid transport to promote embryogenesis in C. elegans","volume":"12","author":"Jeyasimman","year":"2021","journal-title":"Nat Commun"},{"key":"2026060604203623800_gaag031-B16","doi-asserted-by":"crossref","first-page":"154","DOI":"10.1093\/humupd\/dmaa034","article-title":"Molecular genetics of infertility: loss-of-function mutations in humans and corresponding knockout\/mutated mice","volume":"27","author":"Jiao","year":"2021","journal-title":"Hum Reprod Update"},{"key":"2026060604203623800_gaag031-B17","doi-asserted-by":"crossref","first-page":"4975","DOI":"10.1038\/s41598-018-23176-x","article-title":"The evolutionarily conserved genes: Tex37, Ccdc73, Prss55 and Nxt2 are dispensable for fertility in mice","volume":"8","author":"Khan","year":"2018","journal-title":"Sci 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(Lausanne)"},{"key":"2026060604203623800_gaag031-B27","doi-asserted-by":"crossref","first-page":"272","DOI":"10.5534\/wjmh.220186","article-title":"Towards a multi-omics of male infertility","volume":"41","author":"Wagner","year":"2023","journal-title":"World J Mens Health"},{"key":"2026060604203623800_gaag031-B28","doi-asserted-by":"crossref","first-page":"gaaf052","DOI":"10.1093\/molehr\/gaaf052","article-title":"Advances in non-essential testis-enriched genes for human and mouse spermatogenesis","volume":"31","author":"Wei","year":"2025","journal-title":"Mol Hum Reprod"},{"key":"2026060604203623800_gaag031-B29","doi-asserted-by":"crossref","first-page":"1395","DOI":"10.1038\/s41467-022-29109-7","article-title":"CEP128 is involved in spermatogenesis in humans and mice","volume":"13","author":"Zhang","year":"2022","journal-title":"Nat 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