{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T07:23:30Z","timestamp":1790666610971,"version":"4.1.0"},"reference-count":27,"publisher":"Oxford University Press (OUP)","issue":"10","license":[{"start":{"date-parts":[[2019,7,20]],"date-time":"2019-07-20T00:00:00Z","timestamp":1563580800000},"content-version":"vor","delay-in-days":1,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"name":"Francophone Transplant Society"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2019,10,28]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Uterine transplantation from a deceased donor could become an available option for widely treating uterine infertility. However, this procedure requires more precise knowledge about the graft\u2019s tolerance to extended cold ischemia. Here, we sought to assess the uterine metabolic alterations after extended cold ischemic storage in a model of auto-transplantation in ewe. A total of 14 uterine auto-transplantations were performed, divided into 2 groups: 7 after 3\u00a0h of cold ischemia time (CIT) and 7 after 24\u00a0h. Venous uterine blood was collected before uterus retrieval and during reperfusion (30, 60 and 90\u00a0min); thereafter, blood gases, lactate, glucose and amino acids (AAs) were analyzed. Apoptosis analyses were performed before uterus retrieval and following reperfusion in uterus biopsies. A total of 12 uterine auto-transplantations were successfully performed and 7 ewes were alive \u22658\u00a0days after transplantation. After reperfusion, a decrease in pH, a rise of lactate and lactate\/glucose ratio and a delayed decrease of pO2 were found in the 3\u00a0h CIT group. No significant variation of these parameters was observed in the 24\u00a0h CIT group. Significant decreases of AAs were observed during reperfusion and these decreases were more pronounced and concerned a larger number of compounds in the 24\u00a0h CIT group than in the 3\u00a0h CIT group. There was no significant uterine apoptotic signal in either group. Overall, these results suggest that extended CIT storage delayed restoration of aerobic glycolysis and induced an increase in AA requirements of the uterus after reperfusion. However, this biochemical alteration did not reduce success rate for uterine transplantation.<\/jats:p>","DOI":"10.1093\/molehr\/gaz041","type":"journal-article","created":{"date-parts":[[2019,7,11]],"date-time":"2019-07-11T07:09:51Z","timestamp":1562828991000},"page":"647-659","source":"Crossref","is-referenced-by-count":8,"title":["Metabolic alterations of uterine grafts after extended cold ischemic storage: experimental study in ewes"],"prefix":"10.1093","volume":"25","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1826-3031","authenticated-orcid":false,"given":"Yannick","family":"Tholance","sequence":"first","affiliation":[{"name":"Department of Biochemistry and Molecular Genetics, Dupuytren University Hospital, F-87000 Limoges, France"},{"name":"Synaptopathies and Autoantibodies, Institut NeuroMyoGene Institut national de la sant\u00e9 et de la recherche m\u00e9dicale (INSERM) U1217\/Centre national de la recherche scientifique (CNRS) Unit\u00e9s Mixtes de Recherche (UMR) 5310, University of Lyon, University Jean-Monnet, F-42270 Saint-Priest-en-Jarez, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeremy","family":"Tricard","sequence":"additional","affiliation":[{"name":"Department of Thoracic and Cardiovascular Surgery and Angiology, Dupuytren University Hospital, F-87000 Limoges, France"},{"name":"Individual Profiling and Prevention of Risks with Immunosuppressive Therapies and Transplantation, UMR 1248 INSERM, School of Medicine, University of Limoges, F-87000 Limoges, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thierry","family":"Chianea","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Genetics, Dupuytren University Hospital, F-87000 Limoges, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pierre","family":"Marquet","sequence":"additional","affiliation":[{"name":"Individual Profiling and Prevention of Risks with Immunosuppressive Therapies and Transplantation, UMR 1248 INSERM, School of Medicine, University of Limoges, F-87000 Limoges, France"},{"name":"Department of Pharmacology and Toxicology, Dupuytren University Hospital, F-87000 Limoges, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S\u00e9bastien","family":"Ponsonnard","sequence":"additional","affiliation":[{"name":"Elsan, Clinique Ch\u00e9nieux, F-87000 Limoges, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Franck","family":"Sturtz","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Genetics, Dupuytren University Hospital, F-87000 Limoges, France"},{"name":"EA 6309\u2014Myelin Maintenance and Peripheral Neuropathies, School of Medicine, University of Limoges, F-87000 Limoges, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alessandro","family":"Piccardo","sequence":"additional","affiliation":[{"name":"Department of Thoracic and Cardiovascular Surgery and Angiology, Dupuytren University Hospital, F-87000 Limoges, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tristan","family":"Gauthier","sequence":"additional","affiliation":[{"name":"Individual Profiling and Prevention of Risks with Immunosuppressive Therapies and Transplantation, UMR 1248 INSERM, School of Medicine, University of Limoges, F-87000 Limoges, France"},{"name":"Department of Gynecology and Obstetrics, Dupuytren University Hospital, F-87000 Limoges, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2019,7,19]]},"reference":[{"key":"2019103013453058400_ref1","doi-asserted-by":"crossref","first-page":"465","DOI":"10.1097\/TP.0b013e3181b07666","article-title":"Heterotopic uterus transplantation in a swine 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