{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,3]],"date-time":"2026-07-03T00:28:31Z","timestamp":1783038511579,"version":"3.54.6"},"reference-count":52,"publisher":"Oxford University Press (OUP)","issue":"11","license":[{"start":{"date-parts":[[2019,8,28]],"date-time":"2019-08-28T00:00:00Z","timestamp":1566950400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"DOI":"10.13039\/501100004410","name":"Scientific and Technological Research Council of Turkey","doi-asserted-by":"publisher","award":["112O259"],"award-info":[{"award-number":["112O259"]}],"id":[{"id":"10.13039\/501100004410","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2019,11,30]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>In vitro culture under atmospheric oxygen puts embryos under oxidative stress and impairs preimplantation development. However, to what extent this process alters the redox balance in the perinatal period remains largely unknown. The aim of the present study was to examine if the redox balance is altered in the lung tissue of fetuses generated through transfer of mouse embryos exposed to atmospheric oxygen at different stages of development and to determine if this has any effect on lung morphogenesis and gene expression. Two experimental groups (EGs) were generated by transferring in vitro- and in vivo-derived blastocysts to pseudo-pregnant females. In vivo-developed fetuses served as control. Enzymatic\/nonenzymatic antioxidants, malondialdehyde (MDA) levels, total antioxidant capacity, stage of lung development and gene expression were evaluated on day 18 of pregnancy. Weight of fetuses was significantly less in both experimental cohorts (ANOVA, P\u2009&lt;\u20090.001 versus control), associated with delayed lung development, higher amounts of MDA (ANOVA, P\u2009&lt;\u20090.001 versus control) and altered expression of several genes in oxidative stress\/damage pathways. Evidence gathered in the present study indicates that pre-implantation stress caused by culture under atmospheric oxygen, even for a short period of time, leads to fetal growth restriction, impaired lung development and redox balance along with dysregulation of several genes in oxidative stress response. Absence of an EG in which in vitro embryo culture was performed at 5% oxygen and the use of genetically heterogeneous F2 fetuses are the limitations of the study. In any case, the long-term impact of such dramatic changes in the developmental programming of resulting fetuses warrants further investigations.<\/jats:p>","DOI":"10.1093\/molehr\/gaz052","type":"journal-article","created":{"date-parts":[[2019,8,20]],"date-time":"2019-08-20T19:14:45Z","timestamp":1566328485000},"page":"745-754","source":"Crossref","is-referenced-by-count":10,"title":["Transfer of mouse blastocysts exposed to ambient oxygen levels can lead to impaired lung development and redox balance"],"prefix":"10.1093","volume":"25","author":[{"given":"Nedim","family":"Karagen\u00e7","sequence":"first","affiliation":[{"name":"Faculty of Medicine, Department of Medical Genetics, Pamukkale University, Denizli, Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G\u00f6ksel","family":"Do\u011fan","sequence":"additional","affiliation":[{"name":"Faculty of Veterinary Medicine, Department of Histology-Embryology, Adnan Menderes University, Ayd\u0131n, Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kerem","family":"Esmen","sequence":"additional","affiliation":[{"name":"Faculty of Medicine, Department of Basic Medical Sciences, Dokuz Eyl\u00fcl University, \u0130zmir, Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bengi \u00c7\u0131nar","family":"Kul","sequence":"additional","affiliation":[{"name":"Faculty of Veterinary Medicine, Department of Genetics, Ankara University, Ankara, Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hasan","family":"Ye\u015filkaya","sequence":"additional","affiliation":[{"name":"Department of Respiratory Sciences, University of Leicester, Leicester, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mehmet Nurullah","family":"Orman","sequence":"additional","affiliation":[{"name":"Faculty of Medicine, Department of Biostatistics and Medical Informatics, Ege University, \u0130zmir, Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mustafa","family":"Sand\u0131k\u00e7\u0131","sequence":"additional","affiliation":[{"name":"Faculty of Veterinary Medicine, Department of Histology-Embryology, Adnan Menderes University, Ayd\u0131n, Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H\u00fcmeyra","family":"\u00dcnsal","sequence":"additional","affiliation":[{"name":"Faculty of Veterinary Medicine, Department of Physiology, Adnan Menderes University, Ayd\u0131n, Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2074-2450","authenticated-orcid":false,"given":"Levent","family":"Karagen\u00e7","sequence":"additional","affiliation":[{"name":"Faculty of Veterinary Medicine, Department of Histology-Embryology, Adnan Menderes University, Ayd\u0131n, Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2019,8,28]]},"reference":[{"key":"2019112917103519700_ref1","doi-asserted-by":"crossref","first-page":"503","DOI":"10.1016\/j.fertnstert.2006.02.088","article-title":"Oxidative stress in an assisted reproductive technique setting","volume":"86","author":"Agarwal","year":"2006","journal-title":"Fertil Steril"},{"key":"2019112917103519700_ref2","first-page":"59","article-title":"The effect of synchronous and asynchronous egg transfer on foetal weight in mice selected for large and small body size","volume":"37","author":"Aitken","year":"1977","journal-title":"J Embryol Exp Morphol"},{"key":"2019112917103519700_ref3","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1016\/j.jpeds.2017.08.041","article-title":"Can we understand the pathobiology of bronchopulmonary dysplasia?","volume":"190","author":"Alvira","year":"2017","journal-title":"J Pediatr"},{"key":"2019112917103519700_ref4","doi-asserted-by":"crossref","first-page":"484","DOI":"10.1016\/S1472-6483(10)61630-6","article-title":"Oxygen concentration and preimplantation development","volume":"9","author":"Bavister","year":"2004","journal-title":"Reprod Biomed Online"},{"key":"2019112917103519700_ref5","volume-title":"Manipulating the Mouse Embryo. 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