{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T20:07:06Z","timestamp":1792786026054,"version":"4.5.0"},"reference-count":58,"publisher":"Oxford University Press (OUP)","issue":"1","license":[{"start":{"date-parts":[[2019,11,29]],"date-time":"2019-11-29T00:00:00Z","timestamp":1574985600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"DOI":"10.13039\/100012756","name":"Lalor Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100012756","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["HD040793"],"award-info":[{"award-number":["HD040793"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["HD069623"],"award-info":[{"award-number":["HD069623"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Boston Area Diabetes and Endocrinology Research Center","award":["DK57521"],"award-info":[{"award-number":["DK57521"]}]},{"name":"Center for the Study of Inflammatory Bowel Disease","award":["DK43351"],"award-info":[{"award-number":["DK43351"]}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["S10-OD-021577-01"],"award-info":[{"award-number":["S10-OD-021577-01"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2020,1,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>In the epididymis, prevention of autoimmune responses against spermatozoa and simultaneous protection against pathogens is important for male fertility. We have previously shown that mononuclear phagocytes (MPs) are located either in the epididymal interstitium or in close proximity to the epithelium. In the initial segments (IS), these \u2018intraepithelial\u2019 MPs extend slender luminal-reaching projections between epithelial cells. In this study, we performed an in-depth characterisation of MPs isolated from IS, caput\u2013corpus and cauda epididymis of CX3CR1EGFP+\/\u2212 mice that express EGFP in these cells. Flow cytometry analysis revealed region-specific subsets of MPs that express combinations of markers traditionally described in \u2018dendritic cells\u2019 or \u2018macrophages\u2019. RNA sequencing identified distinct transcriptomic signatures in MPs from each region and revealed specific genes involved in inflammatory and anti-inflammatory responses, phagosomal activity and antigen processing and presentation. Functional fluorescent in vivo labelling assays showed that higher percentages of CX3CR1+ MPs that captured and processed antigens were detected in the IS compared to other regions. Confocal microscopy showed that in the IS, caput and corpus, circulatory antigens were internalised and processed by interstitial and intraepithelial MPs. However, in the cauda only interstitial MPs internalised and processed antigens, while intraepithelial MPs did not take up antigens, indicating that all antigens have been captured before they reached the epithelial lining. Cauda MPs may thus confer a stronger protection against blood-borne pathogens compared to proximal regions. By identifying immunoregulatory mechanisms in the epididymis, our study may lead to new therapies for male infertility and epididymitis and identify potential targets for immunocontraception.<\/jats:p>","DOI":"10.1093\/molehr\/gaz059","type":"journal-article","created":{"date-parts":[[2019,10,17]],"date-time":"2019-10-17T15:37:29Z","timestamp":1571326649000},"page":"14-29","source":"Crossref","is-referenced-by-count":52,"title":["Region-specific transcriptomic and functional signatures of mononuclear phagocytes in the epididymis"],"prefix":"10.1093","volume":"26","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0490-9548","authenticated-orcid":false,"given":"Maria A","family":"Battistone","sequence":"first","affiliation":[{"name":"Program in Membrane Biology, Center for Systems Biology and Division of Nephrology, Department of Medicine, Massachusetts General Hospital and Harvard Medical School, Boston, MA, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexandra C","family":"Mendelsohn","sequence":"additional","affiliation":[{"name":"Program in Membrane Biology, Center for Systems Biology and Division of Nephrology, Department of Medicine, Massachusetts General Hospital and Harvard Medical School, Boston, MA, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5402-3687","authenticated-orcid":false,"given":"Raul German","family":"Spallanzani","sequence":"additional","affiliation":[{"name":"Division of Immunology, Department of Microbiology an Immunobiology, Harvard Medical School, Evergrande Center for Immunologic Diseases, and Brigham and Women\u2019s Hospital, Boston, MA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dennis","family":"Brown","sequence":"additional","affiliation":[{"name":"Program in Membrane Biology, Center for Systems Biology and Division of Nephrology, Department of Medicine, Massachusetts General Hospital and Harvard Medical School, Boston, MA, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anil V","family":"Nair","sequence":"additional","affiliation":[{"name":"Program in Membrane Biology, Center for Systems Biology and Division of Nephrology, Department of Medicine, Massachusetts General Hospital and Harvard Medical School, Boston, MA, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6380-8689","authenticated-orcid":false,"given":"Sylvie","family":"Breton","sequence":"additional","affiliation":[{"name":"Program in Membrane Biology, Center for Systems Biology and Division of Nephrology, Department of Medicine, Massachusetts General Hospital and Harvard Medical School, Boston, MA, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2019,11,29]]},"reference":[{"key":"2020013101265052800_ref1","author":"NICHD","year":"2017"},{"key":"2020013101265052800_ref2","doi-asserted-by":"crossref","first-page":"209","DOI":"10.1002\/ar.1092090208","article-title":"Microvasculature of the mouse epididymis, with special reference to fenestrated capillaries localized in the initial segment","volume":"209","author":"Abe","year":"1984","journal-title":"Anat Rec"},{"key":"2020013101265052800_ref3","doi-asserted-by":"crossref","first-page":"18","DOI":"10.1038\/ni.3325","article-title":"The role of the local environment and epigenetics in shaping macrophage identity and their effect on tissue homeostasis","volume":"17","author":"Amit","year":"2016","journal-title":"Nat Immunol"},{"key":"2020013101265052800_ref56","doi-asserted-by":"crossref","first-page":"166","DOI":"10.1093\/bioinformatics\/btu638","article-title":"HTSeq \u2013a Python framework to work with high-throughput sequencing data","volume":"31","author":"Anders","year":"2015","journal-title":"Bioinformatics"},{"key":"2020013101265052800_ref4","doi-asserted-by":"crossref","first-page":"245","DOI":"10.1038\/32588","article-title":"Dendritic cells and the control of immunity","volume":"392","author":"Banchereau","year":"1998","journal-title":"Nature"},{"key":"2020013101265052800_ref6","doi-asserted-by":"crossref","first-page":"545","DOI":"10.1681\/ASN.2017060643","article-title":"Extracellular adenosine stimulates vacuolar ATPase-dependent proton secretion in medullary intercalated cells","volume":"29","author":"Battistone","year":"2018","journal-title":"J Am Soc Nephrol"},{"key":"2020013101265052800_ref5","doi-asserted-by":"crossref","first-page":"1957","DOI":"10.1113\/JP277565","article-title":"Unraveling purinergic regulation in the epididymis: activation of V-ATPase-dependent acidification by luminal ATP and adenosine","volume":"597","author":"Battistone","year":"2019","journal-title":"J Physiol"},{"key":"2020013101265052800_ref58","article-title":"Novel role of proton-secreting epithelial cells in sperm maturation and mucosal immunity","volume":"133","author":"Battistone","year":"2019","journal-title":"J Cell Sci"},{"key":"2020013101265052800_ref7","doi-asserted-by":"crossref","first-page":"270","DOI":"10.1016\/j.it.2014.04.003","article-title":"Intestinal macrophages and dendritic cells: what\u2019s the difference?","volume":"35","author":"Cerovic","year":"2014","journal-title":"Trends Immunol"},{"key":"2020013101265052800_ref8","doi-asserted-by":"crossref","first-page":"153","DOI":"10.1016\/j.immuni.2012.09.018","article-title":"Circulatory antigen processing by mucosal dendritic cells controls CD8(+) T cell activation","volume":"38","author":"Chang","year":"2013","journal-title":"Immunity"},{"key":"2020013101265052800_ref9","doi-asserted-by":"crossref","first-page":"435","DOI":"10.1038\/nri2335","article-title":"Dendritic cells in intestinal immune regulation","volume":"8","author":"Coombes","year":"2008","journal-title":"Nat Rev Immunol"},{"key":"2020013101265052800_ref10","doi-asserted-by":"crossref","first-page":"1757","DOI":"10.1084\/jem.20070590","article-title":"A functionally specialized population of mucosal CD103+ DCs induces Foxp3+ regulatory T cells via a TGF-beta and retinoic acid-dependent mechanism","volume":"204","author":"Coombes","year":"2007","journal-title":"J Exp Med"},{"key":"2020013101265052800_ref11","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1093\/humupd\/dmn055","article-title":"New insights into epididymal biology and function","volume":"15","author":"Cornwall","year":"2009","journal-title":"Hum Reprod Update"},{"key":"2020013101265052800_ref12","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1007\/s00441-015-2270-0","article-title":"Macrophages and dendritic cells in the post-testicular environment","volume":"363","author":"Da Silva","year":"2016","journal-title":"Cell Tissue Res"},{"key":"2020013101265052800_ref13","doi-asserted-by":"crossref","first-page":"653","DOI":"10.1530\/REP-10-0493","article-title":"A dense network of dendritic cells populates the murine epididymis","volume":"141","author":"Da Silva","year":"2011","journal-title":"Reproduction"},{"key":"2020013101265052800_ref14","doi-asserted-by":"crossref","first-page":"591","DOI":"10.4103\/1008-682X.153540","article-title":"Exploring the role of mononuclear phagocytes in the epididymis","volume":"17","author":"Da Silva","year":"2015","journal-title":"Asian J Androl"},{"key":"2020013101265052800_ref55","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1093\/bioinformatics\/bts635","article-title":"STAR: ultrafast universal RNA-seq aligner","volume":"29","author":"Dobin","year":"2013","journal-title":"Bioinformatics"},{"key":"2020013101265052800_ref15","doi-asserted-by":"crossref","first-page":"1059","DOI":"10.1083\/jcb.201004096","article-title":"Clearance of apoptotic cells: implications in health and disease","volume":"189","author":"Elliott","year":"2010","journal-title":"J Cell Biol"},{"key":"2020013101265052800_ref16","doi-asserted-by":"crossref","first-page":"416","DOI":"10.1093\/humupd\/dmy009","article-title":"Infectious, inflammatory and \u2018autoimmune\u2019 male factor infertility: how do rodent models inform clinical practice?","volume":"24","author":"Fijak","year":"2018","journal-title":"Hum Reprod Update"},{"key":"2020013101265052800_ref17","doi-asserted-by":"crossref","first-page":"380","DOI":"10.1002\/(SICI)1097-0185(199707)248:3<380::AID-AR11>3.0.CO;2-L","article-title":"Distribution of leukocytes in the epithelium and interstitium of four regions of the Lewis rat epididymis","volume":"248","author":"Flickinger","year":"1997","journal-title":"Anat Rec"},{"key":"2020013101265052800_ref18","doi-asserted-by":"crossref","first-page":"453","DOI":"10.1038\/nri2784","article-title":"Unravelling mononuclear phagocyte heterogeneity","volume":"10","author":"Geissmann","year":"2010","journal-title":"Nat Rev Immunol"},{"key":"2020013101265052800_ref19","doi-asserted-by":"crossref","first-page":"36","DOI":"10.1111\/imr.12223","article-title":"Macrophage heterogeneity in tissues: phenotypic diversity and functions","volume":"262","author":"Gordon","year":"2014","journal-title":"Immunol Rev"},{"key":"2020013101265052800_ref20","doi-asserted-by":"crossref","first-page":"953","DOI":"10.1038\/nri1733","article-title":"Monocyte and macrophage heterogeneity","volume":"5","author":"Gordon","year":"2005","journal-title":"Nat Rev Immunol"},{"key":"2020013101265052800_ref21","doi-asserted-by":"crossref","first-page":"1327","DOI":"10.1016\/j.cell.2014.11.023","article-title":"Environment drives selection and function of enhancers controlling tissue-specific macrophage identities","volume":"159","author":"Gosselin","year":"2014","journal-title":"Cell"},{"key":"2020013101265052800_ref22","doi-asserted-by":"crossref","DOI":"10.1111\/andr.12637","article-title":"Of vessels and cells: the spatial organization of the epididymal immune system","author":"Guiton","year":"2019","journal-title":"Andrology"},{"key":"2020013101265052800_ref23","doi-asserted-by":"crossref","first-page":"323","DOI":"10.1016\/j.immuni.2011.09.007","article-title":"Dendritic cell and macrophage heterogeneity in vivo","volume":"35","author":"Hashimoto","year":"2011","journal-title":"Immunity"},{"key":"2020013101265052800_ref24","doi-asserted-by":"crossref","first-page":"625","DOI":"10.2164\/jandrol.111.012989","article-title":"Immunophysiology and pathology of inflammation in the testis and epididymis","volume":"32","author":"Hedger","year":"2011","journal-title":"J Androl"},{"key":"2020013101265052800_ref25","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1111\/j.0105-2896.2009.00885.x","article-title":"Origin and functional heterogeneity of non-lymphoid tissue dendritic cells in mice","volume":"234","author":"Helft","year":"2010","journal-title":"Immunol Rev"},{"key":"2020013101265052800_ref26","doi-asserted-by":"crossref","first-page":"355","DOI":"10.1002\/cyto.b.20485","article-title":"Considerations for the control of background fluorescence in clinical flow cytometry","volume":"76","author":"Hulspas","year":"2009","journal-title":"Cytometry B Clin Cytom"},{"key":"2020013101265052800_ref27","doi-asserted-by":"crossref","first-page":"432","DOI":"10.1038\/mi.2008.36","article-title":"Differentiation and heterogeneity in the mononuclear phagocyte system","volume":"1","author":"Hume","year":"2008","journal-title":"Mucosal Immunol"},{"key":"2020013101265052800_ref28","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1016\/j.jri.2005.06.007","article-title":"Tissue microcircumstances for leukocytic infiltration into the testis and epididymis in mice","volume":"67","author":"Itoh","year":"2005","journal-title":"J Reprod Immunol"},{"key":"2020013101265052800_ref29","doi-asserted-by":"crossref","first-page":"349","DOI":"10.1038\/nri.2017.28","article-title":"Monocyte differentiation and antigen-presenting functions","volume":"17","author":"Jakubzick","year":"2017","journal-title":"Nat Rev Immunol"},{"key":"2020013101265052800_ref30","first-page":"849","article-title":"Infertility","volume":"75","author":"Jose-Miller","year":"2007","journal-title":"Am Fam Physician"},{"key":"2020013101265052800_ref31","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0066494","article-title":"Indoleamine 2, 3-dioxygenase 1 (ido 1) is involved in the control of mouse caput epididymis immune environment","volume":"8","author":"Jrad-Lamine","year":"2013","journal-title":"PLoS One"},{"key":"2020013101265052800_ref32","doi-asserted-by":"crossref","first-page":"4106","DOI":"10.1128\/MCB.20.11.4106-4114.2000","article-title":"Analysis of fractalkine receptor CX (3) CR1 function by targeted deletion and green fluorescent protein reporter gene insertion","volume":"20","author":"Jung","year":"2000","journal-title":"Mol Cell Biol"},{"key":"2020013101265052800_ref33","doi-asserted-by":"crossref","first-page":"1312","DOI":"10.1016\/j.cell.2014.11.018","article-title":"Tissue-resident macrophage enhancer landscapes are shaped by the local microenvironment","volume":"159","author":"Lavin","year":"2014","journal-title":"Cell"},{"key":"2020013101265052800_ref34","doi-asserted-by":"crossref","first-page":"e5","DOI":"10.4110\/in.2018.18.e5","article-title":"Tissue-specific role of CX3CR1 expressing immune cells and their relationships with human disease","volume":"18","author":"Lee","year":"2018","journal-title":"Immune Netw"},{"issue":"Pt 2","key":"2020013101265052800_ref35","first-page":"255","article-title":"A quantitative description of the epididymis and its microvasculature: an age-related study in the rat","volume":"180","author":"Markey","year":"1992","journal-title":"J Anat"},{"key":"2020013101265052800_ref36","doi-asserted-by":"crossref","first-page":"13","DOI":"10.12703\/P6-13","article-title":"The M1 and M2 paradigm of macrophage activation: time for reassessment","volume":"6","author":"Martinez","year":"2014","journal-title":"F1000Prime Rep"},{"key":"2020013101265052800_ref37","doi-asserted-by":"crossref","first-page":"595","DOI":"10.1136\/gut.2009.185108","article-title":"Gut CD103+ dendritic cells express indoleamine 2, 3-dioxygenase which influences T regulatory\/T effector cell balance and oral tolerance induction","volume":"59","author":"Matteoli","year":"2010","journal-title":"Gut"},{"key":"2020013101265052800_ref38","doi-asserted-by":"crossref","first-page":"248","DOI":"10.1016\/j.immuni.2013.12.012","article-title":"Oral tolerance can be established via gap junction transfer of fed antigens from CX3CR1(+) macrophages to CD103(+) dendritic cells","volume":"40","author":"Mazzini","year":"2014","journal-title":"Immunity"},{"key":"2020013101265052800_ref39","doi-asserted-by":"crossref","first-page":"169","DOI":"10.1007\/978-1-4939-3606-9_12","article-title":"Protocols for the identification and isolation of antigen-presenting cells in human and mouse tissues","volume":"1423","author":"McGovern","year":"2016","journal-title":"Methods Mol Biol"},{"key":"2020013101265052800_ref40","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1016\/S0165-0378(02)00014-1","article-title":"Basis, diagnosis and treatment of immunological infertility in men","volume":"57","author":"McLachlan","year":"2002","journal-title":"J Reprod Immunol"},{"key":"2020013101265052800_ref41","doi-asserted-by":"crossref","first-page":"687","DOI":"10.1097\/01.mog.0000181710.96904.58","article-title":"Lamina propria dendritic cells in the physiology and pathology of the gastrointestinal tract","volume":"21","author":"Niess","year":"2005","journal-title":"Curr Opin Gastroenterol"},{"key":"2020013101265052800_ref42","doi-asserted-by":"crossref","first-page":"1882","DOI":"10.3389\/fimmu.2018.01882","article-title":"Transforming growth factor beta signaling in dendritic cells is required for immunotolerance to sperm in the epididymis","volume":"9","author":"Pierucci-Alves","year":"2018","journal-title":"Front Immunol"},{"key":"2020013101265052800_ref43","doi-asserted-by":"crossref","first-page":"167","DOI":"10.1016\/0165-0378(94)90019-1","article-title":"Immunology of the testicular excurrent ducts","volume":"26","author":"Pollanen","year":"1994","journal-title":"J Reprod Immunol"},{"key":"2020013101265052800_ref57","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1093\/bioinformatics\/btp616","article-title":"edgeR: a Bioconductor package for differential expression analysis of digital gene expression data","volume":"26","author":"Robinson","year":"2010","journal-title":"Bioinformatics"},{"key":"2020013101265052800_ref44","doi-asserted-by":"crossref","first-page":"4396","DOI":"10.1242\/jcs.148098","article-title":"Breton S. CFTR interacts with ZO-1 to regulate tight junction assembly and epithelial differentiation through the ZONAB pathway","volume":"127","author":"Ruan","year":"2014","journal-title":"J Cell Sci"},{"key":"2020013101265052800_ref45","first-page":"219","author":"Serre","year":"2002"},{"key":"2020013101265052800_ref46","doi-asserted-by":"crossref","first-page":"90","DOI":"10.1095\/biolreprod.113.116681","article-title":"Epithelial basal cells are distinct from dendritic cells and macrophages in the mouse epididymis","volume":"90","author":"Shum","year":"2014","journal-title":"Biol Reprod"},{"key":"2020013101265052800_ref47","doi-asserted-by":"crossref","first-page":"419","DOI":"10.1038\/nature06175","article-title":"Taking dendritic cells into medicine","volume":"449","author":"Steinman","year":"2007","journal-title":"Nature"},{"key":"2020013101265052800_ref48","doi-asserted-by":"crossref","first-page":"685","DOI":"10.1146\/annurev.immunol.21.120601.141040","article-title":"Tolerogenic dendritic cells","volume":"21","author":"Steinman","year":"2003","journal-title":"Annu Rev Immunol"},{"key":"2020013101265052800_ref49","doi-asserted-by":"crossref","first-page":"309","DOI":"10.1002\/aja.1001630404","article-title":"Microvasculature of the mouse testis and excurrent duct system","volume":"163","author":"Suzuki","year":"1982","journal-title":"Am J Anat"},{"key":"2020013101265052800_ref50","doi-asserted-by":"crossref","first-page":"3150","DOI":"10.1002\/eji.201242847","article-title":"CD64 distinguishes macrophages from dendritic cells in the gut and reveals the Th1-inducing role of mesenteric lymph node macrophages during colitis","volume":"42","author":"Tamoutounour","year":"2012","journal-title":"Eur J Immunol"},{"key":"2020013101265052800_ref51","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1111\/j.1749-6632.2010.05746.x","article-title":"Immune regulation by apoptotic cell clearance","volume":"1209","author":"Tanaka","year":"2010","journal-title":"Ann N Y Acad Sci"},{"key":"2020013101265052800_ref52","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1016\/j.jri.2018.01.003","article-title":"Comprehensive overview of murine epididymal mononuclear phagocytes and lymphocytes: unexpected populations arise","volume":"126","author":"Voisin","year":"2018","journal-title":"J Reprod Immunol"},{"key":"2020013101265052800_ref53","doi-asserted-by":"crossref","first-page":"371","DOI":"10.1017\/S0967199400002240","article-title":"Fertility of mammalian spermatozoa: its development and relativity","volume":"2","author":"Yanagimachi","year":"1994","journal-title":"Zygote"},{"key":"2020013101265052800_ref54","doi-asserted-by":"crossref","first-page":"917","DOI":"10.1095\/biolreprod50.4.917","article-title":"Basal cells of the human epididymis--antigenic and ultrastructural similarities to tissue-fixed macrophages","volume":"50","author":"Yeung","year":"1994","journal-title":"Biol Reprod"}],"container-title":["Molecular Human Reproduction"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/academic.oup.com\/molehr\/advance-article-pdf\/doi\/10.1093\/molehr\/gaz059\/31853689\/gaz059.pdf","content-type":"application\/pdf","content-version":"am","intended-application":"syndication"},{"URL":"http:\/\/academic.oup.com\/molehr\/article-pdf\/26\/1\/14\/32265780\/gaz059.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"http:\/\/academic.oup.com\/molehr\/article-pdf\/26\/1\/14\/32265780\/gaz059.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,1,31]],"date-time":"2020-01-31T01:27:17Z","timestamp":1580434037000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/molehr\/article\/26\/1\/14\/5610798"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,11,29]]},"references-count":58,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2019,11,29]]},"published-print":{"date-parts":[[2020,1,1]]}},"URL":"https:\/\/doi.org\/10.1093\/molehr\/gaz059","relation":{},"ISSN":["1460-2407"],"issn-type":[{"value":"1460-2407","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2020,1]]},"published":{"date-parts":[[2019,11,29]]}}}