{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,8]],"date-time":"2026-01-08T08:37:34Z","timestamp":1767861454974,"version":"3.49.0"},"reference-count":0,"publisher":"Oxford University Press (OUP)","license":[{"start":{"date-parts":[[2019,10,25]],"date-time":"2019-10-25T00:00:00Z","timestamp":1571961600000},"content-version":"am","delay-in-days":1,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"DOI":"10.13039\/501100000024","name":"Canadian Institutes of Health Research","doi-asserted-by":"publisher","award":["MOP 133661"],"award-info":[{"award-number":["MOP 133661"]}],"id":[{"id":"10.13039\/501100000024","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000156","name":"Fonds de la recherche en sante du Quebec","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000156","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Montreal General Hospital Foundation"},{"name":"Montreal Children\u2019s Hospital Foundation"},{"name":"Cedars Cancer Foundation RI MUHC \u2013 Desjardins Studentship in Child Health Research"},{"name":"Claude Gagnon Urology Studentship"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Peroxiredoxins (PRDXs) are antioxidant enzymes proven to control the levels of reactive oxygen species (ROS) and to avoid oxidative damage in the spermatozoon. Previously, we have shown that low amounts of PRDXs are associated with male infertility and that PRDX6 is the primary antioxidant defence in human spermatozoa, maintaining survival and DNA integrity (Gong et al., 2012, Fernandez and O\u2019Flaherty, 2018). Oxidative stress can trigger different pathway cascades in the spermatozoa, including truncated apoptosis. It has been reported that the phosphorylation status of phosphoinositide 3-kinase (PI3K) and its target AKT (protein kinase B) prevent the spermatozoon from entering the truncated apoptotic cascade. Here, we aim to study the regulation of the PI3K\/AKT pathway by PRDX6 and assess its role in maintaining sperm viability. Human semen samples were obtained over 1\u00a0year from 20 healthy non-smoking volunteers aged 22\u201330\u00a0years old. Sperm viability, lipid peroxidation and apoptosis-like changes were determined by flow cytometry while phosphorylation of PI3K and AKT substrates were assessed by immunoblotting using anti-phospho-PI3K and anti-phospho-AKT substrates antibodies. We found that the addition of arachidonic acid and lysophosphatidic acid, products of PRDX6 calcium independent phospholipase A2 (Ca2+-iPLA2), prevented loss of sperm viability and maintained the phosphorylation of PI3K. Antioxidant compounds such as D-penicillamine partially prevented the oxidative damage on spermatozoa that led to a reduction of their viability. Thus, other pathways can also participate in sperm survival and be regulated by PRDXs. In conclusion, PRDX6 contributes to the regulation of ROS production and the PI3K\/AKT pathway for the maintenance of sperm survival.<\/jats:p>","DOI":"10.1093\/molehr\/gaz060","type":"journal-article","created":{"date-parts":[[2019,10,20]],"date-time":"2019-10-20T03:09:10Z","timestamp":1571540950000},"source":"Crossref","is-referenced-by-count":10,"title":["Peroxiredoxin 6 regulates the phosphoinositide 3-kinase\/AKT pathway to maintain human sperm viability"],"prefix":"10.1093","author":[{"given":"Maria C","family":"Fernandez","sequence":"first","affiliation":[{"name":"The Research Institute of the McGill University Health Centre, Montreal, Quebec, H4A 3J1, Canada"},{"name":"Department of Surgery (Urology Division), McGill University, Montr\u00e9al, Qu\u00e9bec, H4A 3J1, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alex","family":"Yu","sequence":"additional","affiliation":[{"name":"The Research Institute of the McGill University Health Centre, Montreal, Quebec, H4A 3J1, Canada"},{"name":"Department of Surgery (Urology Division), McGill University, Montr\u00e9al, Qu\u00e9bec, H4A 3J1, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Adel R","family":"Moawad","sequence":"additional","affiliation":[{"name":"The Research Institute of the McGill University Health Centre, Montreal, Quebec, H4A 3J1, Canada"},{"name":"Department of Surgery (Urology Division), McGill University, Montr\u00e9al, Qu\u00e9bec, H4A 3J1, Canada"},{"name":"Department of Theriogenology, Faculty of Veterinary Medicine, Cairo University, Giza, Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cristian","family":"O\u2019Flaherty","sequence":"additional","affiliation":[{"name":"The Research Institute of the McGill University Health Centre, Montreal, Quebec, H4A 3J1, Canada"},{"name":"Department of Surgery (Urology Division), McGill University, Montr\u00e9al, Qu\u00e9bec, H4A 3J1, Canada"},{"name":"Department of Pharmacology and Therapeutics, McGill University, Montr\u00e9al, Qu\u00e9bec, H3G 1Y6, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2019,10,24]]},"container-title":["Molecular Human Reproduction"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/academic.oup.com\/molehr\/advance-article-pdf\/doi\/10.1093\/molehr\/gaz060\/30268532\/gaz060.pdf","content-type":"application\/pdf","content-version":"am","intended-application":"syndication"},{"URL":"http:\/\/academic.oup.com\/molehr\/advance-article-pdf\/doi\/10.1093\/molehr\/gaz060\/30268532\/gaz060.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,10,25]],"date-time":"2019-10-25T10:19:40Z","timestamp":1571998780000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/molehr\/advance-article\/doi\/10.1093\/molehr\/gaz060\/5603786"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,10,24]]},"references-count":0,"URL":"https:\/\/doi.org\/10.1093\/molehr\/gaz060","relation":{},"ISSN":["1460-2407"],"issn-type":[{"value":"1460-2407","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,10,24]]},"article-number":"gaz060"}}