{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T16:14:51Z","timestamp":1790612091980,"version":"4.1.0"},"reference-count":26,"publisher":"Oxford University Press (OUP)","issue":"3","license":[{"start":{"date-parts":[[2022,6,4]],"date-time":"2022-06-04T00:00:00Z","timestamp":1654300800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"DOI":"10.13039\/501100003478","name":"Japanese Ministry of Health, Labour and Welfare","doi-asserted-by":"crossref","award":["20FC1050"],"award-info":[{"award-number":["20FC1050"]}],"id":[{"id":"10.13039\/501100003478","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100009619","name":"Japan Agency for Medical Research and Development","doi-asserted-by":"publisher","award":["21ek0109531h0001"],"award-info":[{"award-number":["21ek0109531h0001"]}],"id":[{"id":"10.13039\/100009619","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2023,4,13]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n               <jats:sec>\n                  <jats:title>Objective<\/jats:title>\n                  <jats:p>To develop a multianalyte assay for the detection of dermatomyositis (DM)-related autoantibodies using immunoprecipitation (IP) combined with immunoblotting (IB).<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Methods<\/jats:title>\n                  <jats:p>Sera from 116 DM patients were subjected to RNA and protein immunoprecipitation assays as well as commercial enzyme-linked immunosorbent assays (ELISAs) for anti-aminoacyl transfer RNA synthetase, anti-melanoma differentiation antigen 5 (MDA5), anti-Mi-2, anti-transcriptional intermediary factor-1\u03b3 (TIF-1\u03b3), and anti-U1 ribonucleoprotein antibodies. The IP\/IB assay was developed by immunoprecipitation of autoantigens from HeLa cell extracts using patient sera, followed by immunoblotting with an antibody against Mi-2, TIF-1\u03b3, OJ, nuclear matrix protein (NXP)-2, MDA5, PM\/Scl, small ubiquitin-like modifier activating enzyme (SAE), or Ku. A multianalyte assay was designed by mixing primary antibodies in the IP\/IB assay.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Results<\/jats:title>\n                  <jats:p>IP assays identified any DM-related autoantibodies in 100 patients (86%), of which 82% were covered by commercial ELISAs, with a false-positive result in two sera and a false-negative result in one serum. The results obtained from the multianalyte IP\/IB assay and \u2018gold-standard\u2019 IP assays were concordant in terms of the presence or absence of anti-MDA5, anti-TIF-1\u03b3, anti-OJ, anti-NXP-2, anti-PM\/Scl, anti-SAE, anti-Mi-2, and anti-Ku antibodies.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Conclusion<\/jats:title>\n                  <jats:p>This multianalyte IP\/IB assay combined with commercial ELISAs is an alternative to \u2018gold-standard\u2019 IP assays for the detection of DM-related autoantibodies.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1093\/mr\/roac056","type":"journal-article","created":{"date-parts":[[2022,6,4]],"date-time":"2022-06-04T08:23:50Z","timestamp":1654331030000},"page":"543-548","source":"Crossref","is-referenced-by-count":14,"title":["A multianalyte assay for the detection of dermatomyositis-related autoantibodies based on immunoprecipitation combined with 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