{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,21]],"date-time":"2026-08-21T12:11:57Z","timestamp":1787314317684,"version":"build-2736575974"},"reference-count":43,"publisher":"Oxford University Press (OUP)","issue":"6","license":[{"start":{"date-parts":[[2024,3,5]],"date-time":"2024-03-05T00:00:00Z","timestamp":1709596800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,10,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>Osteoarthritis (OA) is the most common chronic joint degenerative disease. Herein, we investigated long non-coding RNA Opa-interacting protein 5-antisense transcript 1\u2019s (OIP5-AS1) in regulating mitophagy during OA.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>RNA immunoprecipitation and RNA pull-down verified the relationship between molecules. Cell counting kit-8 detected cell viability. Enzyme-linked immunosorbent assay evaluated inflammatory cytokines secretion. Flow cytometry measured the contents of reactive oxygen species (ROS) and calcium. Immunofluorescence staining analysed TOMM20 and LC3B levels. JC-1 staining was adopted to measure mitochondrial membrane potential. The changes of mitophagy were analysed by transmission electron microscopy.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Lipopolysaccharide (LPS) treatment contributed to the decrease of chondrocyte viability, and calcium level and inhibited mitochondrial membrane potential, while elevating the secretion of inflammatory factors, ROS, and TOMM20 expression. OIP5-AS1 overexpression inhibited LPS-induced chondrocyte injury and activated mitophagy. OIP5-AS1 upregulated the peroxisome proliferator\u2013activated receptor-\u03b3 (PPAR-\u03b3) mRNA level to regulate adenosine monophosphate\u2013activated protein kinase (AMPK)\/v-akt murine thymoma viral oncogene homolog (Akt)\/mammalian target of rapamycin (mTOR) signalling by interacting with FUS. PPAR-\u03b3 overexpression alleviated LPS-induced chondrocyte injury by activating AMPK\/Akt\/mTOR signalling. PPAR-\u03b3 knockdown reversed the promotion of OIP5-AS1 upregulation on mitophagy.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>OIP5-AS1 promotes PPAR-\u03b3 expression to activate the AMPK\/Akt\/mTOR signalling, thereby enhancing mitophagy and alleviating OA progression.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1093\/mr\/roae015","type":"journal-article","created":{"date-parts":[[2024,3,4]],"date-time":"2024-03-04T01:34:35Z","timestamp":1709516075000},"page":"1265-1276","source":"Crossref","is-referenced-by-count":11,"title":["lncRNA OIP5-AS1 promotes mitophagy to alleviate osteoarthritis by upregulating PPAR-\u03b3 to activate the AMPK\/Akt\/mTOR pathway"],"prefix":"10.1093","volume":"34","author":[{"given":"Zhilu","family":"Sun","sequence":"first","affiliation":[{"name":"The First Affiliated Hospital, Emergency Medicine, Hengyang Medical School, University of South China , Hengyang, Hunan Province,","place":["P.R. 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China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu","family":"Liu","sequence":"additional","affiliation":[{"name":"The First Affiliated Hospital, Emergency Medicine, Hengyang Medical School, University of South China , Hengyang, Hunan Province,","place":["P.R. China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing","family":"Liu","sequence":"additional","affiliation":[{"name":"The First Affiliated Hospital, Department of Rehabilitation, Hengyang Medical School, University of South China , Hengyang, Hunan Province,","place":["P.R. 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