{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,22]],"date-time":"2025-12-22T10:14:09Z","timestamp":1766398449552,"version":"3.48.0"},"reference-count":31,"publisher":"Oxford University Press (OUP)","issue":"1","license":[{"start":{"date-parts":[[2025,7,29]],"date-time":"2025-07-29T00:00:00Z","timestamp":1753747200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,12,22]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>Osteonecrosis of the femoral head (ONFH) is a progressive hip disorder often misdiagnosed owing to overlapping symptoms and radiographic findings. MicroRNAs (miRNAs) have emerged as potential diagnostic biomarkers; however, their role in ONFH remains unclear. This study analysed miRNA expression in synovial fluid and hip joint tissues of patients with ONFH to identify diagnostic markers.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Synovial fluid and hip joint tissues were collected from 24 patients with ONFH and 12 patients with osteoarthritis (OA) undergoing total hip arthroplasty. MiRNA expression was assessed using microarray and validated using real-time polymerase chain reaction (PCR). Statistical analyses compared ONFH and OA groups and examined clinical factor correlations. Tissue-specific expression was evaluated in subchondral and cancellous bone, synovia, and labrum.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Real-time PCR revealed significant upregulation of miR-210 (P\u2009&amp;lt;\u2009.01), miR-296, miR-659, and let-7c (P\u2009&amp;lt;\u2009.05) in ONFH synovial fluid compared to that of OA. Expression patterns were independent of clinical factors. MiR-210 was also significantly upregulated in cancellous bone of the reparative zone in patients with ONFH (P\u2009&amp;lt;\u2009.05).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>MiR-210, miR-296, miR-659, and let-7c upregulation in ONFH synovial fluid independent of clinical factors indicates their potential as diagnostic markers. Localized increase in miR-210 in cancellous bone may indicate its role in ONFH pathophysiology.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1093\/mr\/roaf071","type":"journal-article","created":{"date-parts":[[2025,7,28]],"date-time":"2025-07-28T11:27:36Z","timestamp":1753702056000},"page":"144-151","source":"Crossref","is-referenced-by-count":0,"title":["Analysis of microRNA expression in synovial fluid and intra-articular tissues of osteonecrosis of the femoral head"],"prefix":"10.1093","volume":"36","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3191-0894","authenticated-orcid":false,"given":"Hideki","family":"Shozen","sequence":"first","affiliation":[{"name":"Department of Orthopedic Surgery, Hiroshima University Hospital , Hiroshima ,","place":["Japan"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takeshi","family":"Shoji","sequence":"additional","affiliation":[{"name":"Department of Orthopedic Surgery, Hiroshima University Hospital , Hiroshima ,","place":["Japan"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shinichi","family":"Ueki","sequence":"additional","affiliation":[{"name":"Department of Orthopedic Surgery, Hiroshima University Hospital , Hiroshima ,","place":["Japan"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hiroki","family":"Kaneta","sequence":"additional","affiliation":[{"name":"Department of Orthopedic Surgery, Hiroshima University Hospital , Hiroshima ,","place":["Japan"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nobuo","family":"Adachi","sequence":"additional","affiliation":[{"name":"Department of Orthopedic Surgery, Hiroshima University Hospital , Hiroshima ,","place":["Japan"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2025,7,29]]},"reference":[{"key":"2025122205110671800_ref1","doi-asserted-by":"publisher","first-page":"283","DOI":"10.1007\/s00264-007-0320-2","article-title":"Avascular necrosis of the femoral head: inter- and intraobserver variations of Ficat and ARCO classifications","volume":"32","author":"Schmitt-Sody","year":"2008","journal-title":"Int Orthop"},{"key":"2025122205110671800_ref2","first-page":"205","article-title":"Knee osteoarthritis prevalence, risk factors, pathogenesis and features: part I","volume":"2","author":"Heidari","year":"2011","journal-title":"Caspian J Intern Med"},{"key":"2025122205110671800_ref3","doi-asserted-by":"publisher","first-page":"100","DOI":"10.1016\/j.jot.2019.12.004","article-title":"Guidelines for clinical diagnosis and treatment of osteonecrosis of the femoral head in adults (2019 version)","volume":"21","author":"Zhao","year":"2020","journal-title":"J Orthop Translat"},{"key":"2025122205110671800_ref4","first-page":"337","article-title":"Osteonecrosis of the hip: management in the 21st century","volume":"52","author":"Lieberman","year":"2003","journal-title":"Instr Course Lect"},{"key":"2025122205110671800_ref5","doi-asserted-by":"publisher","first-page":"53","DOI":"10.1097\/BLO.0b013e3181591c92","article-title":"Osteonecrosis of the hip: novel approaches to evaluation and treatment","volume":"465","author":"Petrigliano","year":"2007","journal-title":"Clin Orthop Relat Res"},{"key":"2025122205110671800_ref6","doi-asserted-by":"publisher","first-page":"2715","DOI":"10.1007\/s11999-010-1292-x","article-title":"Nationwide epidemiologic survey of idiopathic osteonecrosis of the femoral head","volume":"468","author":"Fukushima","year":"2010","journal-title":"Clin Orthop Relat Res"},{"key":"2025122205110671800_ref7","doi-asserted-by":"publisher","first-page":"808","DOI":"10.1093\/mr\/roab047","article-title":"Epidemiological study of osteonecrosis of the femoral head using the national registry of designated intractable diseases in Japan","volume":"32","author":"Sato","year":"2022","journal-title":"Mod Rheumatol"},{"key":"2025122205110671800_ref8","doi-asserted-by":"publisher","first-page":"e117","DOI":"10.3928\/01477447-20161013-03","article-title":"Radiologic and clinical features of misdiagnosed idiopathic osteonecrosis of the femoral head","volume":"40","author":"Ando","year":"2017","journal-title":"Orthopedics"},{"key":"2025122205110671800_ref9","doi-asserted-by":"publisher","first-page":"150","DOI":"10.1136\/ard.2006.066878","article-title":"Histopathological prevalence of subchondral insufficiency fracture of the femoral head","volume":"67","author":"Yamamoto","year":"2008","journal-title":"Ann Rheum Dis"},{"key":"2025122205110671800_ref10","doi-asserted-by":"publisher","first-page":"599","DOI":"10.3390\/ijms19020599","article-title":"The miRNA mirage: how close are we to finding a non-invasive diagnostic biomarker in endometriosis? 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