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Six intrinsic target elements were analyzed in yeast cells at different cell growth phases cultured in medium with different phosphorus concentrations (0, 7, 14\u00a0mM) to study its effect on cell growth and composition. SC-ICP-MS results were compared with those obtained by the acid digestion and the average ratio was 0.81. The limits of detection of this method were 0.08, 2.54, 12.5, 0.02, 0.02, and 0.08 fg cell\u20131 for Mg, P, K, Mn, Cu, and Zn, respectively. During the exponential growth phase, the cells exhibited higher elemental contents, wider distribution for most elements, and larger cell size in comparison to the stationary growth phase. Phosphorus-free conditions reduced the average P content in single cells of stationary growth phase from 650 to 80 fg. Phosphorus deficiency led to decreasing intracellular concentrations not only of P but also of K and Cu, and to increasing Zn concentration after 48\u00a0h. Mg maintained its concentration at \u223c0.11 fg \u00b5m\u20133 and did not change significantly under the three investigated conditions after 48\u00a0h. Accordingly, Mg content was successfully used to estimate the intracellular concentration of other intrinsic elements in single yeast cells. SC-ICP-MS is suited to determine target elements in single yeast cells, and allows the study of heterogeneity of cell composition and effects of stressors on the elemental content, distribution, and concentrations of intrinsic elements.<\/jats:p>","DOI":"10.1093\/mtomcs\/mfab032","type":"journal-article","created":{"date-parts":[[2021,6,2]],"date-time":"2021-06-02T11:30:48Z","timestamp":1622633448000},"source":"Crossref","is-referenced-by-count":15,"title":["Determination of elemental distribution and evaluation of elemental concentration in single <i>Saccharomyces cerevisiae<\/i> cells using single cell-inductively coupled plasma mass spectrometry"],"prefix":"10.1093","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0774-071X","authenticated-orcid":false,"given":"Wen","family":"Qin","sequence":"first","affiliation":[{"name":"Department of Analytical Chemistry, Helmholtz Centre for Environmental Research\u2014UFZ, Permoserstrasse 15, 04318 Leipzig, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hans-Joachim","family":"St\u00e4rk","sequence":"additional","affiliation":[{"name":"Department of Analytical Chemistry, Helmholtz Centre for Environmental Research\u2014UFZ, Permoserstrasse 15, 04318 Leipzig, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Susann","family":"M\u00fcller","sequence":"additional","affiliation":[{"name":"Department of Environmental Microbiology, Helmholtz Centre for Environmental Research\u2014UFZ, Permoserstrasse 15, 04318 Leipzig, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1606-0764","authenticated-orcid":false,"given":"Thorsten","family":"Reemtsma","sequence":"additional","affiliation":[{"name":"Department of Analytical Chemistry, Helmholtz Centre for Environmental Research\u2014UFZ, Permoserstrasse 15, 04318 Leipzig, Germany"},{"name":"Institute of Analytical Chemistry, University of Leipzig, Linn\u00e9strasse 3, 04103 Leipzig, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3184-2599","authenticated-orcid":false,"given":"Stephan","family":"Wagner","sequence":"additional","affiliation":[{"name":"Department of Analytical Chemistry, Helmholtz Centre for Environmental Research\u2014UFZ, Permoserstrasse 15, 04318 Leipzig, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2021,6,4]]},"reference":[{"key":"2021120813090813500_bib1","doi-asserted-by":"crossref","first-page":"247","DOI":"10.1016\/S0010-8545(00)00324-6","article-title":"The distribution of elements in cells","volume":"200\u2013202","author":"Williams","year":"2000","journal-title":"Coord. 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