{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T03:13:21Z","timestamp":1790738001240,"version":"4.1.0"},"reference-count":58,"publisher":"Oxford University Press (OUP)","issue":"5","license":[{"start":{"date-parts":[[2022,2,1]],"date-time":"2022-02-01T00:00:00Z","timestamp":1643673600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"DOI":"10.13039\/501100004794","name":"Centre National de la Recherche Scientifique","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004794","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2022,5,25]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Iron\u2013sulfur (Fe\u2013S) clusters are inorganic ubiquitous and ancient cofactors. Fe\u2013S-bound proteins contribute to most cellular processes, including DNA replication and integrity, genetic expression and regulation, metabolism, biosynthesis, and most bioenergetics systems. Also, Fe\u2013S proteins hold a great biotechnological potential in metabolite and chemical production, including antibiotics. From classic biophysics and spectroscopy methodologies to recent development in bioinformatics, including structural modeling and chemoproteomics, our capacity to predict and identify Fe\u2013S proteins has spectacularly increased over the recent years. Here, these developments are presented and collectively used to update the composition of Escherichia coli Fe\u2013S proteome, for which we predict 181 occurrences, i.e. 40 more candidates than in our last catalog, and equivalent to 4% of its total proteome. Besides, Fe\u2013S clusters can be targeted by redox active compounds or reactive oxygen and nitrosative species, and even be destabilized by contaminant metals. Accordingly, we discuss how cells handle damaged Fe\u2013S proteins, i.e. degradation, recycling, or repair.<\/jats:p>","DOI":"10.1093\/mtomcs\/mfac022","type":"journal-article","created":{"date-parts":[[2022,3,28]],"date-time":"2022-03-28T03:06:18Z","timestamp":1648436778000},"source":"Crossref","is-referenced-by-count":28,"title":["The Fe\u2013S proteome of <i>Escherichia coli<\/i>: prediction, function, and fate"],"prefix":"10.1093","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9567-2437","authenticated-orcid":false,"given":"Marine","family":"L\u00e9non","sequence":"first","affiliation":[{"name":"Institut Pasteur, Universit\u00e9 Paris Cit\u00e9, CNRS UMR6047, Stress Adaptation and Metabolism Unit, Department of Microbiology , F-75015 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rodrigo","family":"Arias-Cart\u00edn","sequence":"additional","affiliation":[{"name":"Institut Pasteur, Universit\u00e9 Paris Cit\u00e9, CNRS UMR6047, Stress Adaptation and Metabolism Unit, Department of Microbiology , F-75015 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fr\u00e9d\u00e9ric","family":"Barras","sequence":"additional","affiliation":[{"name":"Institut Pasteur, Universit\u00e9 Paris Cit\u00e9, CNRS UMR6047, Stress Adaptation and Metabolism Unit, Department of Microbiology , F-75015 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2022,3,29]]},"reference":[{"issue":"1","key":"2024052508125827900_bib1","doi-asserted-by":"crossref","first-page":"2","DOI":"10.1007\/s007750050002","article-title":"Iron-Sulfur Proteins: Ancient Structures, Still Full of Surprises","volume":"5","author":"Beinert","year":"2000","journal-title":"JBIC"},{"issue":"1","key":"2024052508125827900_bib2","doi-asserted-by":"crossref","first-page":"424","DOI":"10.1002\/anie.202011211","article-title":"Structural Evidence for a [4Fe-5S] Intermediate in the Non-Redox Desulfuration of Thiouracil","volume":"60","author":"Zhou","year":"2021","journal-title":"Angew. 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