{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,31]],"date-time":"2026-07-31T03:44:43Z","timestamp":1785469483793,"version":"3.56.0"},"reference-count":39,"publisher":"Oxford University Press (OUP)","issue":"10","license":[{"start":{"date-parts":[[2022,1,1]],"date-time":"2022-01-01T00:00:00Z","timestamp":1640995200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2022,10,20]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Metal and redox homeostasis in cyanobacteria is tightly controlled to preserve the photosynthetic machinery from mismetallation and minimize cell damage. This control is mainly taken by FUR (ferric uptake regulation) proteins. FurC works as the PerR (peroxide response) paralog in Anabaena sp. PCC7120. Despite its importance, this regulator remained poorly characterized. Although FurC lacks the typical CXXC motifs present in FUR proteins, it contains a tightly bound zinc per subunit. FurC: Zn stoichiometrically binds zinc and manganese in a second site, manganese being more efficient in the binding of FurC: Zn to its DNA target PprxA. Oligomerization analyses of FurC: Zn evidence the occurrence of different aggregates ranging from dimers to octamers. Notably, intermolecular disulfide bonds are not involved in FurC: Zn dimerization, dimer being the most reduced form of the protein. Oligomerization of dimers occurs upon oxidation of thiols by H2O2 or diamide and can be reversed by 1,4-Dithiothreitol (DTT). Irreversible inactivation of the regulator occurs by metal catalyzed oxidation promoted by ferrous iron. However, inactivation upon oxidation with H2O2 in the absence of iron was reverted by addition of DTT. Comparison of models for FurC: Zn dimers and tetramers obtained using AlphaFold Colab and SWISS-MODEL allowed to infer the residues forming both metal-binding sites and to propose the involvement of Cys86 in reversible tetramer formation. Our results decipher the existence of two levels of inactivation of FurC: Zn of Anabaena sp. PCC7120, a reversible one through disulfide-formed FurC: Zn tetramers and the irreversible metal catalyzed oxidation. This additional reversible regulation may be specific of cyanobacteria.<\/jats:p>","DOI":"10.1093\/mtomcs\/mfac077","type":"journal-article","created":{"date-parts":[[2022,10,6]],"date-time":"2022-10-06T20:12:39Z","timestamp":1665087159000},"source":"Crossref","is-referenced-by-count":6,"title":["Metal binding and oligomerization properties of FurC (PerR) from <i>Anabaena<\/i> sp. PCC7120: an additional layer of regulation?"],"prefix":"10.1093","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1960-2672","authenticated-orcid":false,"given":"Cristina","family":"Sarasa-Buisan","sequence":"first","affiliation":[{"name":"Departamento de Bioqu\u00edmica y Biolog\u00eda Molecular y Celular e Instituto de Biocomputaci\u00f3n y F\u00edsica de Sistemas Complejos (Bifi), Universidad de Zaragoza , 50009-Zaragoza , Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Etienne","family":"Emonot","sequence":"additional","affiliation":[{"name":"Universit\u00e9 Grenoble Alpes, CNRS CEA, IRIG-LCBM , 38000 Grenoble , France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9047-0046","authenticated-orcid":false,"given":"Marta","family":"Mart\u00ednez-J\u00falvez","sequence":"additional","affiliation":[{"name":"Departamento de Bioqu\u00edmica y Biolog\u00eda Molecular y Celular e Instituto de Biocomputaci\u00f3n y F\u00edsica de Sistemas Complejos (Bifi), Universidad de Zaragoza , 50009-Zaragoza , Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emma","family":"Sevilla","sequence":"additional","affiliation":[{"name":"Departamento de Bioqu\u00edmica y Biolog\u00eda Molecular y Celular e Instituto de Biocomputaci\u00f3n y F\u00edsica de Sistemas Complejos (Bifi), Universidad de Zaragoza , 50009-Zaragoza , Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adri\u00e1n","family":"Vel\u00e1zquez-Campoy","sequence":"additional","affiliation":[{"name":"Departamento de Bioqu\u00edmica y Biolog\u00eda Molecular y Celular e Instituto de Biocomputaci\u00f3n y F\u00edsica de Sistemas Complejos (Bifi), Universidad de Zaragoza , 50009-Zaragoza , Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Serge","family":"Crouzy","sequence":"additional","affiliation":[{"name":"Universit\u00e9 Grenoble Alpes, CNRS CEA, IRIG-LCBM , 38000 Grenoble , France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M Teresa","family":"Bes","sequence":"additional","affiliation":[{"name":"Departamento de Bioqu\u00edmica y Biolog\u00eda Molecular y Celular e Instituto de Biocomputaci\u00f3n y F\u00edsica de Sistemas Complejos (Bifi), Universidad de Zaragoza , 50009-Zaragoza , Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Isabelle","family":"Michaud-Soret","sequence":"additional","affiliation":[{"name":"Universit\u00e9 Grenoble Alpes, CNRS CEA, IRIG-LCBM , 38000 Grenoble , France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8644-4574","authenticated-orcid":false,"given":"Mar\u00eda F","family":"Fillat","sequence":"additional","affiliation":[{"name":"Departamento de Bioqu\u00edmica y Biolog\u00eda Molecular y Celular e Instituto de Biocomputaci\u00f3n y F\u00edsica de Sistemas Complejos (Bifi), Universidad de Zaragoza , 50009-Zaragoza , Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2022,10,6]]},"reference":[{"key":"2024013108254236700_bib1","doi-asserted-by":"publisher","first-page":"108770","DOI":"10.1016\/j.abb.2021.108770","article-title":"Fur-like proteins: beyond the ferric uptake regulator (Fur) paralog","volume":"701","author":"Sevilla","year":"2021","journal-title":"Arch. 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