{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T23:58:27Z","timestamp":1791244707456,"version":"4.1.0"},"reference-count":0,"publisher":"Oxford University Press (OUP)","license":[{"start":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T00:00:00Z","timestamp":1791158400000},"content-version":"am","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Staphylococcus aureus and Escherichia coli remain major concerns in global public health, posing clinical challenges due to their adaptability, virulence, and biofilm-mediated persistence. The continued emergence of drug-resistant strains necessitates the development of new antimicrobial compounds. In this study, we evaluated the multifunctional potential of a hetero-ligated, thiolato-bridged dinuclear metallacyclic Mn(I)-based CO-releasing compound (S-MnC), analysing its antibacterial, antibiofilm, molecular, and target-binding efficacies against S. aureus and E. coli. The antibacterial potential of S-MnC was confirmed by a significant decrease in bacterial growth, biofilm biomass, matrix integrity, motility, and structural integrity. Our results further confirmed its anti-adhesive potential, demonstrating the depletion of intracellular free thiols and an increase in reactive oxygen species. Additionally, S-MnC displayed safe hemolysis levels. ADMET analysis indicated a good balance of drug-likeness properties. Molecular docking studies suggested potential binding sites of S-MnC to molecular targets such as bacterial DNA Gyrase B, SaeR, SaeS, and SarA of S. aureus, as well as DNA Gyrase B and SdiA of E. coli, suggesting the antivirulence mechanisms mediated by S-MnC. In this study, we report, for the first time, the antibacterial and antibiofilm activities of S-MnC against both Gram-negative, E. coli, and Gram-positive, S. aureus, and its binding affinity to multiple molecular and virulence targets.<\/jats:p>","DOI":"10.1093\/mtomcs\/mfag031","type":"journal-article","created":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T11:27:47Z","timestamp":1791113267000},"source":"Crossref","is-referenced-by-count":0,"title":["Pharmacokinetic, Antimicrobial, Antibiofilm, and Virulence Target Binding Propensities of a Hetero-Ligated Thiolato-Bridged Dinuclear Metallacyclic Manganese(I)-based CO-Releasing Molecule"],"prefix":"10.1093","author":[{"given":"Aswathy","family":"Anil","sequence":"first","affiliation":[{"name":"School of Life Sciences, Pondicherry University Department of Biotechnology, , Kalapet, Puducherry , , 605014","place":["India"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meyappan","family":"Vadivel","sequence":"additional","affiliation":[{"name":"School of Life Sciences, Pondicherry University Department of Biotechnology, , Kalapet, Puducherry , , 605014","place":["India"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-2978-7769","authenticated-orcid":false,"given":"Sriram","family":"Shankar","sequence":"additional","affiliation":[{"name":"School of Life Sciences, Pondicherry University Department of Biotechnology, , Kalapet, Puducherry , , 605014","place":["India"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Subhashree Subhasmita","family":"Nayak","sequence":"additional","affiliation":[{"name":"School of Life Sciences, Pondicherry University Department of Bioinformatics, , Kalapet, Puducherry , , 605014","place":["India"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ramadas","family":"Krishna","sequence":"additional","affiliation":[{"name":"School of Life Sciences, Pondicherry University Department of Bioinformatics, , Kalapet, Puducherry , , 605014","place":["India"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Udit","family":"Kumar","sequence":"additional","affiliation":[{"name":"School of Physical, Chemical and Applied Sciences, Pondicherry University Department of Chemistry, , Kalapet, Puducherry , , 605014","place":["India"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bala","family":"Manimaran","sequence":"additional","affiliation":[{"name":"School of Physical, Chemical and Applied Sciences, Pondicherry University Department of Chemistry, , Kalapet, Puducherry , , 605014","place":["India"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2161-803X","authenticated-orcid":false,"given":"Natarajan","family":"Sakthivel","sequence":"additional","affiliation":[{"name":"School of Life Sciences, Pondicherry University Department of Biotechnology, , Kalapet, Puducherry , , 605014","place":["India"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2026,10,5]]},"container-title":["Metallomics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/metallomics\/advance-article-pdf\/doi\/10.1093\/mtomcs\/mfag031\/71606349\/mfag031.pdf","content-type":"application\/pdf","content-version":"am","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/metallomics\/advance-article-pdf\/doi\/10.1093\/mtomcs\/mfag031\/71606349\/mfag031.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T23:40:16Z","timestamp":1791243616000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/metallomics\/advance-article\/doi\/10.1093\/mtomcs\/mfag031\/8865297"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,10,5]]},"references-count":0,"URL":"https:\/\/doi.org\/10.1093\/mtomcs\/mfag031","relation":{},"ISSN":["1756-591X"],"issn-type":[{"value":"1756-591X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,10,5]]},"article-number":"mfag031"}}