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Resistance to both groups can be mediated by pentapeptide repeat proteins (PRPs). Despite long-term studies, the mechanism of action of these protective PRPs is not known. We show that a PRP, QnrB1 provides specific protection against fluoroquinolones, which strictly requires ATP hydrolysis by gyrase. QnrB1 binds to the GyrB protein and stimulates ATPase activity of the isolated N-terminal ATPase domain of GyrB (GyrB43). We probed the QnrB1 binding site using site-specific incorporation of a photoreactive amino acid and mapped the crosslinks to the GyrB43 protein. We propose a model in which QnrB1 binding allosterically promotes dissociation of the fluoroquinolone molecule from the cleavage complex.<\/jats:p>","DOI":"10.1093\/nar\/gkaa1266","type":"journal-article","created":{"date-parts":[[2021,1,6]],"date-time":"2021-01-06T17:27:05Z","timestamp":1609954025000},"page":"1581-1596","source":"Crossref","is-referenced-by-count":16,"title":["Pentapeptide repeat protein QnrB1 requires ATP hydrolysis to rejuvenate poisoned gyrase complexes"],"prefix":"10.1093","volume":"49","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9932-9073","authenticated-orcid":false,"given":"\u0141ukasz","family":"Mazurek","sequence":"first","affiliation":[{"name":"Malopolska Centre of Biotechnology, Jagiellonian University, Krakow, Poland"},{"name":"Postgraduate School of Molecular Medicine, Warsaw, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9325-3207","authenticated-orcid":false,"given":"Dmitry","family":"Ghilarov","sequence":"additional","affiliation":[{"name":"Malopolska Centre of Biotechnology, Jagiellonian University, Krakow, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9276-7764","authenticated-orcid":false,"given":"Elizabeth","family":"Michalczyk","sequence":"additional","affiliation":[{"name":"Malopolska Centre of Biotechnology, Jagiellonian University, Krakow, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2288-8616","authenticated-orcid":false,"given":"Zuzanna","family":"Pakosz","sequence":"additional","affiliation":[{"name":"Malopolska Centre of Biotechnology, Jagiellonian University, Krakow, Poland"},{"name":"Postgraduate School of Molecular Medicine, Warsaw, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2829-6395","authenticated-orcid":false,"given":"Mikhail","family":"Metelev","sequence":"additional","affiliation":[{"name":"Institute of Gene Biology, Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8206-1661","authenticated-orcid":false,"given":"Wojciech","family":"Czyszczo\u0144","sequence":"additional","affiliation":[{"name":"Malopolska Centre of Biotechnology, Jagiellonian University, Krakow, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0353-6156","authenticated-orcid":false,"given":"Karolina","family":"Wawro","sequence":"additional","affiliation":[{"name":"Malopolska Centre of Biotechnology, Jagiellonian University, Krakow, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Iraj","family":"Behroz","sequence":"additional","affiliation":[{"name":"Institute of Biological Chemistry, Technische Universit\u00e4t Berlin, Berlin, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9225-5534","authenticated-orcid":false,"given":"Svetlana","family":"Dubiley","sequence":"additional","affiliation":[{"name":"Institute of Gene Biology, Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7027-2069","authenticated-orcid":false,"given":"Roderich D","family":"S\u00fcssmuth","sequence":"additional","affiliation":[{"name":"Institute of Biological Chemistry, Technische Universit\u00e4t Berlin, Berlin, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0994-9928","authenticated-orcid":false,"given":"Jonathan G","family":"Heddle","sequence":"additional","affiliation":[{"name":"Malopolska Centre of Biotechnology, Jagiellonian University, Krakow, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2021,1,12]]},"reference":[{"key":"2021022200002218400_B1","doi-asserted-by":"crossref","first-page":"827","DOI":"10.1038\/nrm3228","article-title":"All tangled up: how cells direct, manage and exploit topoisomerase function","volume":"12","author":"Vos","year":"2011","journal-title":"Nat. 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