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Its genome encodes the \u2018large\u2019 L protein, an essential enzyme that has polymerase, capping and methyltransferase activities. The methyltransferase activity leads to RNA co-transcriptional modifications at the N7 position of the cap structure and at the 2\u2032-O position of the first transcribed nucleotide. Unlike other Mononegavirales viruses, the Ebola virus methyltransferase also catalyses 2\u2032-O-methylation of adenosines located within the RNA sequences. Herein, we report the crystal structure at 1.8 \u00c5 resolution of the Ebola virus methyltransferase domain bound to a fragment of a camelid single-chain antibody. We identified structural determinants and key amino acids specifically involved in the internal adenosine-2\u2032-O-methylation from cap-related methylations. These results provide the first high resolution structure of an ebolavirus L protein domain, and the framework to investigate the effects of epitranscriptomic modifications and to design possible antiviral drugs against the Filoviridae family.<\/jats:p>","DOI":"10.1093\/nar\/gkaa1276","type":"journal-article","created":{"date-parts":[[2020,12,23]],"date-time":"2020-12-23T15:20:00Z","timestamp":1608736800000},"page":"1737-1748","source":"Crossref","is-referenced-by-count":21,"title":["First insights into the structural features of Ebola virus methyltransferase activities"],"prefix":"10.1093","volume":"49","author":[{"given":"Coralie","family":"Valle","sequence":"first","affiliation":[{"name":"AFMB, CNRS, Universit\u00e9 Aix-Marseille, UMR 7257, Case 925, 163 Avenue de Luminy, 13288 Marseille Cedex 09, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4662-6872","authenticated-orcid":false,"given":"Baptiste","family":"Martin","sequence":"additional","affiliation":[{"name":"AFMB, CNRS, Universit\u00e9 Aix-Marseille, UMR 7257, Case 925, 163 Avenue de Luminy, 13288 Marseille Cedex 09, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8351-4992","authenticated-orcid":false,"given":"Fran\u00e7ois","family":"Ferron","sequence":"additional","affiliation":[{"name":"AFMB, CNRS, Universit\u00e9 Aix-Marseille, UMR 7257, Case 925, 163 Avenue de Luminy, 13288 Marseille Cedex 09, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"V\u00e9ronique","family":"Roig-Zamboni","sequence":"additional","affiliation":[{"name":"AFMB, CNRS, Universit\u00e9 Aix-Marseille, UMR 7257, Case 925, 163 Avenue de Luminy, 13288 Marseille Cedex 09, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aline","family":"Desmyter","sequence":"additional","affiliation":[{"name":"AFMB, CNRS, Universit\u00e9 Aix-Marseille, UMR 7257, Case 925, 163 Avenue de Luminy, 13288 Marseille Cedex 09, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fran\u00e7oise","family":"Debart","sequence":"additional","affiliation":[{"name":"IBMM, UMR 5247 CNRS, Universit\u00e9 de Montpellier, ENSCM, Montpellier, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean-Jacques","family":"Vasseur","sequence":"additional","affiliation":[{"name":"IBMM, UMR 5247 CNRS, Universit\u00e9 de Montpellier, ENSCM, Montpellier, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4290-8247","authenticated-orcid":false,"given":"Bruno","family":"Canard","sequence":"additional","affiliation":[{"name":"AFMB, CNRS, Universit\u00e9 Aix-Marseille, UMR 7257, Case 925, 163 Avenue de Luminy, 13288 Marseille Cedex 09, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bruno","family":"Coutard","sequence":"additional","affiliation":[{"name":"Unit\u00e9 des Virus \u00c9mergents (UVE: Aix-Marseille Univ-IRD 190-Inserm, 1207-IHU M\u00e9diterran\u00e9e Infection) Marseille, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6046-024X","authenticated-orcid":false,"given":"Etienne","family":"Decroly","sequence":"additional","affiliation":[{"name":"AFMB, CNRS, Universit\u00e9 Aix-Marseille, UMR 7257, Case 925, 163 Avenue de Luminy, 13288 Marseille Cedex 09, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2021,1,27]]},"reference":[{"key":"2021022123594188600_B1","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1016\/j.coviro.2016.11.013","article-title":"Human transmission of Ebola virus","volume":"22","author":"Lawrence","year":"2017","journal-title":"Curr. 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