{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T05:22:23Z","timestamp":1790054543612,"version":"4.0.1"},"reference-count":53,"publisher":"Oxford University Press (OUP)","issue":"16","license":[{"start":{"date-parts":[[2020,7,31]],"date-time":"2020-07-31T00:00:00Z","timestamp":1596153600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100006477","name":"National Taiwan University","doi-asserted-by":"publisher","award":["NTU-CC-108L892004"],"award-info":[{"award-number":["NTU-CC-108L892004"]}],"id":[{"id":"10.13039\/501100006477","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100007225","name":"Ministry of Science and Technology","doi-asserted-by":"publisher","award":["MOST 105-2314-B-002-073"],"award-info":[{"award-number":["MOST 105-2314-B-002-073"]}],"id":[{"id":"10.13039\/100007225","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100007225","name":"Ministry of Science and Technology","doi-asserted-by":"publisher","award":["MOST 108-2321-B-002-054"],"award-info":[{"award-number":["MOST 108-2321-B-002-054"]}],"id":[{"id":"10.13039\/100007225","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100007225","name":"Ministry of Science and Technology","doi-asserted-by":"publisher","award":["MOST 107-2113-M-002-010"],"award-info":[{"award-number":["MOST 107-2113-M-002-010"]}],"id":[{"id":"10.13039\/100007225","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2020,9,18]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Microcephalin 1 (MCPH1) was identified from genetic mutations in patients with primary autosomal recessive microcephaly. In response to DNA double-strand breaks (DSBs), MCPH1 forms damage-induced foci and recruits BRCA2\u2013RAD51 complex, a key component of the DSB repair machinery for homologous recombination (HR), to damage sites. Accordingly, the efficiency of HR is significantly attenuated upon depletion of MCPH1. The biochemical characteristics of MCPH1 and its functional interaction with the HR machinery had remained unclear due to lack of highly purified MCPH1 recombinant protein for functional study. Here, we established a mammalian expression system to express and purify MCPH1 protein. We show that MCPH1 is a bona fide DNA-binding protein and provide direct biochemical analysis of this MCPH family protein. Furthermore, we reveal that MCPH1 directly interacts with RAD51 at multiple contact points, providing evidence for how MCPH1 physically engages with the HR machinery. Importantly, we demonstrate that MCPH1 enhances the stability of RAD51 on single-strand DNA, a prerequisite step for RAD51-mediated recombination. Single-molecule tethered particle motion analysis showed a \u223c2-fold increase in the lifetime of RAD51\u2013ssDNA filaments in the presence of MCPH1. Thus, our study demonstrates direct crosstalk between microcephaly protein MCPH1 and the recombination component RAD51 for DSB repair.<\/jats:p>","DOI":"10.1093\/nar\/gkaa636","type":"journal-article","created":{"date-parts":[[2020,7,22]],"date-time":"2020-07-22T19:24:27Z","timestamp":1595445867000},"page":"9135-9146","source":"Crossref","is-referenced-by-count":14,"title":["Microcephaly family protein MCPH1 stabilizes RAD51 filaments"],"prefix":"10.1093","volume":"48","author":[{"given":"Hao-Yen","family":"Chang","sequence":"first","affiliation":[{"name":"Institute of Biochemical Sciences, National Taiwan University, No. 1, Sec. 4, Roosevelt Road, Taipei\u00a010617, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chia-Yi","family":"Lee","sequence":"additional","affiliation":[{"name":"Institute of Biochemical Sciences, National Taiwan University, No. 1, Sec. 4, Roosevelt Road, Taipei\u00a010617, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chih-Hao","family":"Lu","sequence":"additional","affiliation":[{"name":"Department of Chemistry, National Taiwan University, No. 1, Sec. 4, Roosevelt Road, Taipei\u00a010617, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Chemistry, National Taiwan University, No. 1, Sec. 4, Roosevelt Road, Taipei\u00a010617, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Han-Lin","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Chemistry, National Taiwan University, No. 1, Sec. 4, Roosevelt Road, Taipei\u00a010617, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hsin-Yi","family":"Yeh","sequence":"additional","affiliation":[{"name":"Institute of Biochemical Sciences, National Taiwan University, No. 1, Sec. 4, Roosevelt Road, Taipei\u00a010617, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5614-3727","authenticated-orcid":false,"given":"Hung-Wen","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Chemistry, National Taiwan University, No. 1, Sec. 4, Roosevelt Road, Taipei\u00a010617, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9229-8729","authenticated-orcid":false,"given":"Peter","family":"Chi","sequence":"additional","affiliation":[{"name":"Institute of Biochemical Sciences, National Taiwan University, No. 1, Sec. 4, Roosevelt Road, Taipei\u00a010617, Taiwan"},{"name":"Institute of Biological Chemistry, Academia Sinica, 128 Academia Road, Section 2, Nankang, Taipei 11529, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2020,7,31]]},"reference":[{"key":"2020091720045985600_B1","doi-asserted-by":"crossref","first-page":"136","DOI":"10.1086\/341283","article-title":"Identification of microcephalin, a protein implicated in determining the size of the human brain","volume":"71","author":"Jackson","year":"2002","journal-title":"Am. 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Treat."},{"key":"2020091720045985600_B5","doi-asserted-by":"crossref","first-page":"184","DOI":"10.1016\/j.gene.2012.12.088","article-title":"MCPH1 protein expression and polymorphisms are associated with risk of breast cancer","volume":"517","author":"Jo","year":"2013","journal-title":"Gene"},{"key":"2020091720045985600_B6","doi-asserted-by":"crossref","first-page":"S424","DOI":"10.1245\/s10434-012-2715-6","article-title":"Frequent alterations of MCPH1 and ATM are associated with primary breast carcinoma: clinical and prognostic implications","volume":"20","author":"Bhattacharya","year":"2013","journal-title":"Ann. Surg Oncol."},{"key":"2020091720045985600_B7","doi-asserted-by":"crossref","first-page":"e1000826","DOI":"10.1371\/journal.pgen.1000826","article-title":"BRIT1\/MCPH1 is essential for mitotic and meiotic recombination DNA repair and maintaining genomic stability in mice","volume":"6","author":"Liang","year":"2010","journal-title":"PLoS Genet."},{"key":"2020091720045985600_B8","doi-asserted-by":"crossref","first-page":"1325","DOI":"10.1038\/ncb2342","article-title":"MCPH1 regulates the neuroprogenitor division mode by coupling the centrosomal cycle with mitotic entry through the Chk1-Cdc25 pathway","volume":"13","author":"Gruber","year":"2011","journal-title":"Nat. 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