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The enhancer E\u03b1 plays an essential role in Tcra rearrangement by establishing a recombination centre in the J\u03b1 array and a chromatin hub for interactions between V\u03b1 and J\u03b1 genes. But the mechanism of the E\u03b1 and its downstream CTCF binding site (here named EACBE) in dynamic chromatin regulation is unknown. The Hi-C data showed that the EACBE is located at the sub-TAD boundary which separates the Tcra\u2013Tcrd locus and the downstream region including the Dad1 gene. The EACBE is required for long-distance regulation of the E\u03b1 on the proximal V\u03b1 genes, and its deletion impaired the Tcra rearrangement. We also noticed that the EACBE and E\u03b1 regulate the genes in the downstream sub-TAD via asymmetric chromatin extrusion. This study provides a new insight into the role of CTCF binding sites at TAD boundaries in gene regulation.<\/jats:p>","DOI":"10.1093\/nar\/gkaa711","type":"journal-article","created":{"date-parts":[[2020,8,20]],"date-time":"2020-08-20T19:09:52Z","timestamp":1597950592000},"page":"9621-9636","source":"Crossref","is-referenced-by-count":23,"title":["A role of the CTCF binding site at enhancer E\u03b1 in the dynamic chromatin organization of the Tcra\u2013Tcrd locus"],"prefix":"10.1093","volume":"48","author":[{"given":"Hao","family":"Zhao","sequence":"first","affiliation":[{"name":"Guangdong Provincial Key Laboratory of Tumor Immunotherapy, Cancer Research Institute, School of Basic Medical Sciences, Southern Medical University, Guangzhou, Guangdong 510515, P.R. 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