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Such biomolecular condensates have various biological functions and have been linked to disease. The protein Fused in Sarcoma (FUS) undergoes LLPS and mutations in FUS have been causally linked to the motor neuron disease Amyotrophic Lateral Sclerosis (ALS-FUS). LLPS followed by aggregation of cytoplasmic FUS has been proposed to be a crucial disease mechanism. However, it is currently unclear how LLPS impacts the behaviour of FUS in cells, e.g. its interactome. Hence, we developed a method allowing for the purification of LLPS FUS-containing droplets from cell lysates. We observe substantial alterations in the interactome, depending on its biophysical state. While non-LLPS FUS interacts mainly with factors involved in pre-mRNA processing, LLPS FUS predominantly binds to proteins involved in chromatin remodelling and DNA damage repair. Interestingly, also mitochondrial factors are strongly enriched with LLPS FUS, providing a potential explanation for the observed changes in mitochondrial gene expression in mouse models of ALS-FUS. In summary, we present a methodology to investigate the interactomes of phase separating proteins and provide evidence that LLPS shapes the FUS interactome with implications for function and disease.<\/jats:p>","DOI":"10.1093\/nar\/gkab582","type":"journal-article","created":{"date-parts":[[2021,6,28]],"date-time":"2021-06-28T07:09:23Z","timestamp":1624864163000},"page":"7713-7731","source":"Crossref","is-referenced-by-count":121,"title":["The phase separation-dependent FUS interactome reveals nuclear and cytoplasmic function of liquid\u2013liquid phase separation"],"prefix":"10.1093","volume":"49","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4885-3550","authenticated-orcid":false,"given":"Stefan","family":"Reber","sequence":"first","affiliation":[{"name":"United Kingdom Dementia Research Institute Centre at King's College London, Institute of Psychiatry, Psychology and Neuroscience, King's College London, Maurice Wohl Clinical Neuroscience Institute, London, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8381-7830","authenticated-orcid":false,"given":"Daniel","family":"Jutzi","sequence":"additional","affiliation":[{"name":"United Kingdom Dementia Research Institute Centre at King's College London, Institute of Psychiatry, Psychology and Neuroscience, King's College London, Maurice Wohl Clinical Neuroscience Institute, London, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Helen","family":"Lindsay","sequence":"additional","affiliation":[{"name":"Department of Mathematics, \u00c9cole polytechnique f\u00e9d\u00e9rale de Lausanne (EPFL), Lausanne, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1293-0630","authenticated-orcid":false,"given":"Anny","family":"Devoy","sequence":"additional","affiliation":[{"name":"United Kingdom Dementia Research Institute Centre at King's College London, Institute of Psychiatry, Psychology and Neuroscience, King's College London, Maurice Wohl Clinical Neuroscience Institute, London, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8818-7671","authenticated-orcid":false,"given":"Jonas","family":"Mechtersheimer","sequence":"additional","affiliation":[{"name":"United Kingdom Dementia Research Institute Centre at King's College London, Institute of Psychiatry, Psychology and Neuroscience, King's College London, Maurice Wohl Clinical Neuroscience Institute, London, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3368-4261","authenticated-orcid":false,"given":"Brunno Rocha","family":"Levone","sequence":"additional","affiliation":[{"name":"Department of Biotechnology and Biosciences, University of Milano-Bicocca, Milan, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6892-1496","authenticated-orcid":false,"given":"Michal","family":"Domanski","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Biochemistry, University of Bern, Bern, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eva","family":"Bentmann","sequence":"additional","affiliation":[{"name":"Biomedical Center (BMC), Cell Biology, Ludwig Maximilians University Munich, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9260-2775","authenticated-orcid":false,"given":"Dorothee","family":"Dormann","sequence":"additional","affiliation":[{"name":"Biomedical Center (BMC), Cell Biology, Ludwig Maximilians University Munich, Germany"},{"name":"Munich Cluster for Systems Neurology (SyNergy), Munich, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0657-1368","authenticated-orcid":false,"given":"Oliver","family":"M\u00fchlemann","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Biochemistry, University of Bern, Bern, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3584-4417","authenticated-orcid":false,"given":"Silvia M L","family":"Barabino","sequence":"additional","affiliation":[{"name":"Department of Biotechnology and Biosciences, University of Milano-Bicocca, Milan, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3264-9800","authenticated-orcid":false,"given":"Marc-David","family":"Ruepp","sequence":"additional","affiliation":[{"name":"United Kingdom Dementia Research Institute Centre at King's College London, Institute of Psychiatry, Psychology and Neuroscience, King's College London, 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