{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,25]],"date-time":"2026-02-25T17:59:05Z","timestamp":1772042345938,"version":"3.50.1"},"reference-count":45,"publisher":"Oxford University Press (OUP)","issue":"15","license":[{"start":{"date-parts":[[2021,7,30]],"date-time":"2021-07-30T00:00:00Z","timestamp":1627603200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2018YFA0107304"],"award-info":[{"award-number":["2018YFA0107304"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81201181"],"award-info":[{"award-number":["81201181"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004731","name":"Natural Science Foundation of Zhejiang Province","doi-asserted-by":"publisher","award":["2017C37176"],"award-info":[{"award-number":["2017C37176"]}],"id":[{"id":"10.13039\/501100004731","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100007835","name":"Wenzhou Medical University","doi-asserted-by":"publisher","award":["J02-20190201"],"award-info":[{"award-number":["J02-20190201"]}],"id":[{"id":"10.13039\/100007835","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Lin He's Academician Workstation of New Medicine and Clinical Translation","award":["17331209"],"award-info":[{"award-number":["17331209"]}]},{"name":"Lin He's Academician Workstation of New Medicine and Clinical Translation","award":["18331105"],"award-info":[{"award-number":["18331105"]}]},{"DOI":"10.13039\/501100012152","name":"National Postdoctoral Program for Innovative Talents","doi-asserted-by":"publisher","award":["BX20200348"],"award-info":[{"award-number":["BX20200348"]}],"id":[{"id":"10.13039\/501100012152","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002858","name":"China Postdoctoral Science Foundation","doi-asserted-by":"publisher","award":["2020M681407"],"award-info":[{"award-number":["2020M681407"]}],"id":[{"id":"10.13039\/501100002858","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Wenzhou City Key Innovation Team of Reproductive Genetics","award":["C20170007"],"award-info":[{"award-number":["C20170007"]}]},{"name":"Wenzhou city","award":["2021Y1893"],"award-info":[{"award-number":["2021Y1893"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2021,9,7]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Cytosine base editor (CBE) enables targeted C-to-T conversions at single base-pair resolution and thus has potential therapeutic applications in humans. However, the low efficiency of the system limits practical use of this approach. We reported a high-throughput human cells-based reporter system that can be harnessed for quickly measuring editing activity of CBE. Screening of 1813 small-molecule compounds resulted in the identification of Ricolinostat (an HDAC6 inhibitor) that can enhance the efficiency of BE3 in human cells (2.45- to 9.21-fold improvement). Nexturastat A, another HDAC6 inhibitor, could also increase BE3-mediated gene editing by 2.18- to 9.95-fold. Ricolinostat and Nexturastat A also boost base editing activity of the other CBE variants (BE4max, YE1-BE4max, evoAPOBEC1-BE4max and SpRY-CBE4max, up to 8.32-fold). Meanwhile, combined application of BE3 and Ricolinostat led to\u00a0&amp;gt;3-fold higher efficiency of correcting a pathogenic mutation in ABCA4 gene related to Stargardt disease in human cells. Moreover, we demonstrated that our strategy could be applied for efficient generation of mouse models through direct zygote injection and base editing in primary human T cells. Our study provides a new strategy to improve the activity and specificity of CBE in human cells. Ricolinostat and Nexturastat A augment the effectiveness and applicability of CBE.<\/jats:p>","DOI":"10.1093\/nar\/gkab645","type":"journal-article","created":{"date-parts":[[2021,7,17]],"date-time":"2021-07-17T19:08:09Z","timestamp":1626548889000},"page":"8974-8986","source":"Crossref","is-referenced-by-count":13,"title":["Small-molecule compounds boost genome-editing efficiency of cytosine base editor"],"prefix":"10.1093","volume":"49","author":[{"given":"Tianyuan","family":"Zhao","sequence":"first","affiliation":[{"name":"School of Ophthalmology and Optometry, Eye Hospital, Wenzhou Medical University, State Key Laboratory and Key Laboratory of Vision Science, Ministry of Health and Zhejiang Provincial Key Laboratory of Ophthalmology and Optometry, Wenzhou, Zhejiang, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qing","family":"Li","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Cell Biology, Shanghai Key Laboratory of Molecular Andrology, Shanghai Institute of Biochemistry and Cell Biology, Center for Excellence in Molecular Cell Science, Chinese Academy of Sciences, Shanghai, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chenchen","family":"Zhou","sequence":"additional","affiliation":[{"name":"School of Ophthalmology and Optometry, Eye Hospital, Wenzhou Medical University, State Key Laboratory and Key Laboratory of Vision Science, Ministry of Health and Zhejiang Provincial Key Laboratory of Ophthalmology and Optometry, Wenzhou, Zhejiang, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiujuan","family":"Lv","sequence":"additional","affiliation":[{"name":"School of Ophthalmology and Optometry, Eye Hospital, Wenzhou Medical University, State Key Laboratory and Key Laboratory of Vision Science, Ministry of Health and Zhejiang Provincial Key Laboratory of Ophthalmology and Optometry, Wenzhou, Zhejiang, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hongyan","family":"Liu","sequence":"additional","affiliation":[{"name":"School of Ophthalmology and Optometry, Eye Hospital, Wenzhou Medical University, State Key Laboratory and Key Laboratory of Vision Science, Ministry of Health and Zhejiang Provincial Key Laboratory of Ophthalmology and Optometry, Wenzhou, Zhejiang, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tianxiang","family":"Tu","sequence":"additional","affiliation":[{"name":"School of Ophthalmology and Optometry, Eye Hospital, Wenzhou Medical University, State Key Laboratory and Key Laboratory of Vision Science, Ministry of Health and Zhejiang Provincial Key Laboratory of Ophthalmology and Optometry, Wenzhou, Zhejiang, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Na","family":"Tang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, University of Chinese Academy of Sciences, Beijing, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yanbo","family":"Cheng","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Cell Biology, Shanghai Key Laboratory of Molecular Andrology, Shanghai Institute of Biochemistry and Cell Biology, Center for Excellence in Molecular Cell Science, Chinese Academy of Sciences, Shanghai, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaoyu","family":"Liu","sequence":"additional","affiliation":[{"name":"School of Ophthalmology and Optometry, Eye Hospital, Wenzhou Medical University, State Key Laboratory and Key Laboratory of Vision Science, Ministry of Health and Zhejiang Provincial Key Laboratory of Ophthalmology and Optometry, Wenzhou, Zhejiang, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Changbao","family":"Liu","sequence":"additional","affiliation":[{"name":"The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Junzhao","family":"Zhao","sequence":"additional","affiliation":[{"name":"The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zongming","family":"Song","sequence":"additional","affiliation":[{"name":"Henan Eye Hospital, Henan Provincial People's Hospital and People's Hospital of Zhengzhou University, People's Hospital of Henan University, Zhengzhou, Henan, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Haoyi","family":"Wang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, University of Chinese Academy of Sciences, Beijing, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jinsong","family":"Li","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Cell Biology, Shanghai Key Laboratory of Molecular Andrology, Shanghai Institute of Biochemistry and Cell Biology, Center for Excellence in Molecular Cell Science, Chinese Academy of Sciences, Shanghai, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9600-8407","authenticated-orcid":false,"given":"Feng","family":"Gu","sequence":"additional","affiliation":[{"name":"School of Ophthalmology and Optometry, Eye Hospital, Wenzhou Medical University, State Key Laboratory and Key Laboratory of Vision Science, Ministry of Health and Zhejiang Provincial Key Laboratory of Ophthalmology and Optometry, Wenzhou, Zhejiang, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2021,7,30]]},"reference":[{"key":"2021090700053323100_B1","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1038\/nrd.2016.238","article-title":"Cornerstones of CRISPR\u2013Cas in drug discovery and therapy","volume":"16","author":"Fellmann","year":"2017","journal-title":"Nat. Rev. Drug Discov."},{"key":"2021090700053323100_B2","doi-asserted-by":"crossref","first-page":"661","DOI":"10.1038\/s41580-020-00288-9","article-title":"Applications of CRISPR-Cas in agriculture and plant biotechnology","volume":"21","author":"Zhu","year":"2020","journal-title":"Nat. Rev. Mol. Cell Biol."},{"key":"2021090700053323100_B3","doi-asserted-by":"crossref","first-page":"419","DOI":"10.1038\/s41591-019-0343-4","article-title":"Single-dose CRISPR\u2013Cas9 therapy extends lifespan of mice with Hutchinson\u2013Gilford progeria syndrome","volume":"25","author":"Beyret","year":"2019","journal-title":"Nat. Med."},{"key":"2021090700053323100_B4","doi-asserted-by":"crossref","first-page":"420","DOI":"10.1038\/nature17946","article-title":"Programmable editing of a target base in genomic DNA without double-stranded DNA cleavage","volume":"533","author":"Komor","year":"2016","journal-title":"Nature"},{"key":"2021090700053323100_B5","doi-asserted-by":"crossref","first-page":"eaay9101","DOI":"10.1126\/scitranslmed.aay9101","article-title":"In vivo base editing restores sensory transduction and transiently improves auditory function in a mouse model of recessive deafness","volume":"12","author":"Yeh","year":"2020","journal-title":"Sci. Transl. Med."},{"key":"2021090700053323100_B6","doi-asserted-by":"crossref","first-page":"91","DOI":"10.1089\/crispr.2019.0001","article-title":"Development and application of base editors","volume":"2","author":"Yang","year":"2019","journal-title":"CRISPR J."},{"key":"2021090700053323100_B7","doi-asserted-by":"crossref","first-page":"1315","DOI":"10.1038\/s41556-018-0202-4","article-title":"CRISPR-Cas9-mediated base-editing screening in mice identifies DND1 amino acids that are critical for primordial germ cell development","volume":"20","author":"Li","year":"2018","journal-title":"Nat. Cell Biol."},{"key":"2021090700053323100_B8","doi-asserted-by":"crossref","first-page":"371","DOI":"10.1038\/nbt.3803","article-title":"Increasing the genome-targeting scope and precision of base editing with engineered Cas9-cytidine deaminase fusions","volume":"35","author":"Kim","year":"2017","journal-title":"Nat. Biotechnol."},{"key":"2021090700053323100_B9","doi-asserted-by":"crossref","first-page":"1070","DOI":"10.1038\/s41587-019-0193-0","article-title":"Continuous evolution of base editors with expanded target compatibility and improved activity","volume":"37","author":"Thuronyi","year":"2019","journal-title":"Nat. Biotechnol."},{"key":"2021090700053323100_B10","doi-asserted-by":"crossref","first-page":"3612","DOI":"10.1038\/s41467-019-11562-6","article-title":"Expanding C-T base editing toolkit with diversified cytidine deaminases","volume":"10","author":"Cheng","year":"2019","journal-title":"Nat. Commun."},{"key":"2021090700053323100_B11","doi-asserted-by":"crossref","first-page":"620","DOI":"10.1038\/s41587-020-0414-6","article-title":"Evaluation and minimization of Cas9-independent off-target DNA editing by cytosine base editors","volume":"38","author":"Doman","year":"2020","journal-title":"Nat. Biotechnol."},{"key":"2021090700053323100_B12","doi-asserted-by":"crossref","first-page":"600","DOI":"10.1038\/s41592-020-0832-x","article-title":"A rationally engineered cytosine base editor retains high on-target activity while reducing both DNA and RNA off-target effects","volume":"17","author":"Zuo","year":"2020","journal-title":"Nat. Methods"},{"key":"2021090700053323100_B13","doi-asserted-by":"crossref","first-page":"626","DOI":"10.1038\/s41587-019-0134-y","article-title":"Circularly permuted and PAM-modified Cas9 variants broaden the targeting scope of base editors","volume":"37","author":"Huang","year":"2019","journal-title":"Nat. Biotechnol."},{"key":"2021090700053323100_B14","doi-asserted-by":"crossref","first-page":"276","DOI":"10.1038\/s41587-018-0011-0","article-title":"Engineered CRISPR-Cas12a variants with increased activities and improved targeting ranges for gene, epigenetic and base editing","volume":"37","author":"Kleinstiver","year":"2019","journal-title":"Nat. Biotechnol."},{"key":"2021090700053323100_B15","doi-asserted-by":"crossref","first-page":"107723","DOI":"10.1016\/j.celrep.2020.107723","article-title":"Cas12a base editors induce efficient and specific editing with low DNA damage response","volume":"31","author":"Wang","year":"2020","journal-title":"Cell Rep."},{"key":"2021090700053323100_B16","doi-asserted-by":"crossref","first-page":"861","DOI":"10.1038\/s41587-020-0535-y","article-title":"A dual-deaminase CRISPR base editor enables concurrent adenine and cytosine editing","volume":"38","author":"Gr\u00fcnewald","year":"2020","journal-title":"Nat. Biotechnol."},{"key":"2021090700053323100_B17","doi-asserted-by":"crossref","first-page":"2338","DOI":"10.1038\/s41467-018-04768-7","article-title":"Efficient generation of mouse models of human diseases via ABE- and BE-mediated base editing","volume":"9","author":"Liu","year":"2018","journal-title":"Nat. Commun."},{"key":"2021090700053323100_B18","doi-asserted-by":"crossref","first-page":"543","DOI":"10.1038\/nbt.3198","article-title":"Increasing the efficiency of homology-directed repair for CRISPR-Cas9-induced precise gene editing in mammalian cells","volume":"33","author":"Chu","year":"2015","journal-title":"Nat. Biotechnol."},{"key":"2021090700053323100_B19","doi-asserted-by":"crossref","first-page":"10548","DOI":"10.1038\/ncomms10548","article-title":"RS-1 enhances CRISPR\/Cas9- and TALEN-mediated knock-in efficiency","volume":"7","author":"Song","year":"2016","journal-title":"Nat. Commun."},{"key":"2021090700053323100_B20","doi-asserted-by":"crossref","first-page":"15848","DOI":"10.1073\/pnas.0808046105","article-title":"A chemical compound that stimulates the human homologous recombination protein RAD51","volume":"105","author":"Jayathilaka","year":"2008","journal-title":"PNAS"},{"key":"2021090700053323100_B21","doi-asserted-by":"crossref","first-page":"1067","DOI":"10.1016\/j.cell.2019.04.009","article-title":"A high-throughput platform to identify small-molecule inhibitors of CRISPR-Cas9","volume":"177","author":"Maji","year":"2019","journal-title":"Cell"},{"key":"2021090700053323100_B22","doi-asserted-by":"crossref","first-page":"5405","DOI":"10.1038\/srep05405","article-title":"Comparison of non-canonical PAMs for CRISPR\/Cas9-mediated DNA cleavage in human cells","volume":"4","author":"Zhang","year":"2014","journal-title":"Sci. Rep."},{"key":"2021090700053323100_B23","doi-asserted-by":"crossref","first-page":"659","DOI":"10.1016\/j.stem.2013.10.016","article-title":"Correction of a genetic disease in mouse via use of CRISPR-Cas9","volume":"13","author":"Wu","year":"2013","journal-title":"Cell stem cell"},{"key":"2021090700053323100_B24","doi-asserted-by":"crossref","first-page":"2114","DOI":"10.1093\/bioinformatics\/btu170","article-title":"Trimmomatic: a flexible trimmer for Illumina sequence data","volume":"30","author":"Bolger","year":"2014","journal-title":"Bioinformatics"},{"key":"2021090700053323100_B25","doi-asserted-by":"crossref","first-page":"e133977","DOI":"10.1172\/jci.insight.133977","article-title":"TGF-\u03b2 inhibition via CRISPR promotes the long-term efficacy of CAR T cells against solid tumors","volume":"5","author":"Tang","year":"2020","journal-title":"JCI Insight"},{"key":"2021090700053323100_B26","doi-asserted-by":"crossref","first-page":"101610","DOI":"10.1016\/j.scr.2019.101610","article-title":"Generation of a homozygous HDAC6 knockout human embryonic stem cell line by CRISPR\/Cas9 editing","volume":"41","author":"Xie","year":"2019","journal-title":"Stem Cell Res."},{"key":"2021090700053323100_B27","doi-asserted-by":"crossref","first-page":"e3000747","DOI":"10.1371\/journal.pbio.3000747","article-title":"High-fidelity SaCas9 identified by directional screening in human cells","volume":"18","author":"Xie","year":"2020","journal-title":"PLoS Biol."},{"key":"2021090700053323100_B28","doi-asserted-by":"crossref","first-page":"11295","DOI":"10.1093\/nar\/gkx783","article-title":"A \u2018new lease of life\u2019: FnCpf1 possesses DNA cleavage activity for genome editing in human cells","volume":"45","author":"Tu","year":"2017","journal-title":"Nucleic Acids Res."},{"key":"2021090700053323100_B29","doi-asserted-by":"crossref","first-page":"580","DOI":"10.1016\/j.omtn.2020.03.004","article-title":"Development of a Simple and Quick Method to Assess Base Editing in Human Cells","volume":"20","author":"Lv","year":"2020","journal-title":"Mol. Ther. Nucleic Acids"},{"key":"2021090700053323100_B30","doi-asserted-by":"crossref","first-page":"438","DOI":"10.1038\/nbt.3811","article-title":"Precise base editing in rice, wheat and maize with a Cas9-cytidine deaminase fusion","volume":"35","author":"Zong","year":"2017","journal-title":"Nat. Biotechnol."},{"key":"2021090700053323100_B31","doi-asserted-by":"crossref","first-page":"378","DOI":"10.1016\/j.omtn.2017.04.018","article-title":"CRISPR\/Cas9-loxP-mediated gene editing as a novel site-specific genetic manipulation tool","volume":"7","author":"Yang","year":"2017","journal-title":"Mol. Ther. Nucleic Acids"},{"key":"2021090700053323100_B32","doi-asserted-by":"crossref","first-page":"2579","DOI":"10.1182\/blood-2011-10-387365","article-title":"Preclinical activity, pharmacodynamic, and pharmacokinetic properties of a selective HDAC6 inhibitor, ACY-1215, in combination with bortezomib in multiple myeloma","volume":"119","author":"Santo","year":"2012","journal-title":"Blood"},{"key":"2021090700053323100_B33","doi-asserted-by":"crossref","first-page":"843","DOI":"10.1038\/nbt.4172","article-title":"Improving cytidine and adenine base editors by expression optimization and ancestral reconstruction","volume":"36","author":"Koblan","year":"2018","journal-title":"Nat. Biotechnol."},{"key":"2021090700053323100_B34","doi-asserted-by":"crossref","first-page":"290","DOI":"10.1126\/science.aba8853","article-title":"Unconstrained genome targeting with near-PAMless engineered CRISPR-Cas9 variants","volume":"368","author":"Walton","year":"2020","journal-title":"Science"},{"key":"2021090700053323100_B35","doi-asserted-by":"crossref","first-page":"275","DOI":"10.1038\/s41586-019-1314-0","article-title":"Off-target RNA mutation induced by DNA base editing and its elimination by mutagenesis","volume":"571","author":"Zhou","year":"2019","journal-title":"Nature"},{"key":"2021090700053323100_B36","doi-asserted-by":"crossref","first-page":"e95528","DOI":"10.1371\/journal.pone.0095528","article-title":"Molecular diagnosis of putative Stargardt disease by capture next generation sequencing","volume":"9","author":"Zhang","year":"2014","journal-title":"PLoS One"},{"key":"2021090700053323100_B37","doi-asserted-by":"crossref","first-page":"10437","DOI":"10.1073\/pnas.1512503112","article-title":"Generation of knock-in primary human T cells using Cas9 ribonucleoproteins","volume":"112","author":"Schumann","year":"2015","journal-title":"Proc. Natl. Acad. Sci. U.S.A."},{"key":"2021090700053323100_B38","doi-asserted-by":"crossref","first-page":"1317","DOI":"10.1038\/s41587-020-0555-7","article-title":"CHANGE-seq reveals genetic and epigenetic effects on CRISPR\u2013Cas9 genome-wide activity","volume":"38","author":"Lazzarotto","year":"2020","journal-title":"Nat. Biotechnol."},{"key":"2021090700053323100_B39","doi-asserted-by":"crossref","first-page":"464","DOI":"10.1038\/nature24644","article-title":"Programmable base editing of A\u2022T to G\u2022C in genomic DNA without DNA cleavage","volume":"551","author":"Gaudelli","year":"2017","journal-title":"Nature"},{"key":"2021090700053323100_B40","doi-asserted-by":"crossref","first-page":"2390","DOI":"10.1093\/nar\/gkab052","article-title":"Small-molecule inhibitors of histone deacetylase improve CRISPR-based adenine base editing","volume":"49","author":"Shin","year":"2021","journal-title":"Nucleic Acids Res."},{"key":"2021090700053323100_B41","doi-asserted-by":"crossref","first-page":"748","DOI":"10.1038\/nchembio.2140","article-title":"Structural insights into HDAC6 tubulin deacetylation and its selective inhibition","volume":"12","author":"Miyake","year":"2016","journal-title":"Nat. Chem. Biol."},{"key":"2021090700053323100_B42","doi-asserted-by":"crossref","first-page":"22516","DOI":"10.1074\/jbc.M113.489328","article-title":"Inhibition of HDAC6 deacetylase activity increases its binding with microtubules and suppresses microtubule dynamic instability in MCF-7 cells","volume":"288","author":"Asthana","year":"2013","journal-title":"J. Biol. Chem."},{"key":"2021090700053323100_B43","doi-asserted-by":"crossref","first-page":"1841","DOI":"10.1038\/mt.2008.190","article-title":"Microtubule acetylation through HDAC6 inhibition results in increased transfection efficiency","volume":"16","author":"Vaughan","year":"2008","journal-title":"Mol. Ther."},{"key":"2021090700053323100_B44","doi-asserted-by":"crossref","first-page":"5813","DOI":"10.1074\/jbc.RA118.006374","article-title":"HDAC6 regulates DNA damage response via deacetylating MLH1","volume":"294","author":"Zhang","year":"2019","journal-title":"J. Biol. Chem."},{"key":"2021090700053323100_B45","doi-asserted-by":"crossref","first-page":"eaao4774","DOI":"10.1126\/sciadv.aao4774","article-title":"Improved base excision repair inhibition and bacteriophage Mu Gam protein yields C:G-to-T:A base editors with higher efficiency and product purity","volume":"3","author":"Komor","year":"2017","journal-title":"Sci. Adv."}],"container-title":["Nucleic Acids Research"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/academic.oup.com\/nar\/article-pdf\/49\/15\/8974\/40083045\/gkab645.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"http:\/\/academic.oup.com\/nar\/article-pdf\/49\/15\/8974\/40083045\/gkab645.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,9,7]],"date-time":"2021-09-07T00:10:38Z","timestamp":1630973438000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/nar\/article\/49\/15\/8974\/6331672"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,7,30]]},"references-count":45,"journal-issue":{"issue":"15","published-online":{"date-parts":[[2021,7,30]]},"published-print":{"date-parts":[[2021,9,7]]}},"URL":"https:\/\/doi.org\/10.1093\/nar\/gkab645","relation":{},"ISSN":["0305-1048","1362-4962"],"issn-type":[{"value":"0305-1048","type":"print"},{"value":"1362-4962","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2021,9,7]]},"published":{"date-parts":[[2021,7,30]]}}}