{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,2]],"date-time":"2026-07-02T13:18:50Z","timestamp":1782998330673,"version":"3.54.5"},"reference-count":47,"publisher":"Oxford University Press (OUP)","issue":"15","license":[{"start":{"date-parts":[[2021,8,6]],"date-time":"2021-08-06T00:00:00Z","timestamp":1628208000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001691","name":"JSPS","doi-asserted-by":"publisher","award":["26000007"],"award-info":[{"award-number":["26000007"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Scientific Research","award":["19H00924"],"award-info":[{"award-number":["19H00924"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2021,9,7]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Small-molecules interacting with particular RNAs and modulating their functions are vital tools for RNA-targeting drug discovery. Considering the substantial distribution of the internal loops involving two contiguous cytosines opposite to a single-nucleotide base (Y\/CC; Y\u00a0=\u00a0C, U or A) within the biologically significant functional RNAs, developing small-molecule probes targeting Y\/CC sites should provide profound insight into their functions and roles in biochemical processes. Herein, we report ANP77 as the small-molecule probe for sensing RNA internal loop of Y\/CC motifs and molecules binding to the motifs. The Y\/CC motifs interact with ANP77 via the formation of a 1:1 complex and quench the fluorescence of ANP77. The flanking sequence-dependent binding to C\/CC and U\/CC sites was assessed by fluorometric screening, provided the binding heat maps. The quenching phenomena of ANP77 fluorescence was confirmed with intrinsic potential drug target pre-miR-1908. Finally, the binding-dependent fluorescence quenching of ANP77 was utilized in the fluorescence indicator displacement assay to demonstrate the potential of ANP77 as an indicator by using the RNA-binding drugs, risdiplam and branaplam.<\/jats:p>","DOI":"10.1093\/nar\/gkab650","type":"journal-article","created":{"date-parts":[[2021,8,4]],"date-time":"2021-08-04T11:53:44Z","timestamp":1628078024000},"page":"8462-8470","source":"Crossref","is-referenced-by-count":18,"title":["A small-molecule fluorescence probe ANP77 for sensing RNA internal loop of C, U and A\/CC motifs and their binding molecules"],"prefix":"10.1093","volume":"49","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9885-4885","authenticated-orcid":false,"given":"Bimolendu","family":"Das","sequence":"first","affiliation":[{"name":"Department of Regulatory Bioorganic Chemistry, SANKEN (The Institute of Scientific and Industrial Research), Osaka University, 8-1 Mihogaoka, Ibaraki, Osaka 567-0047, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2073-7272","authenticated-orcid":false,"given":"Asako","family":"Murata","sequence":"additional","affiliation":[{"name":"Department of Regulatory Bioorganic Chemistry, SANKEN (The Institute of Scientific and Industrial Research), Osaka University, 8-1 Mihogaoka, Ibaraki, Osaka 567-0047, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1705-5265","authenticated-orcid":false,"given":"Kazuhiko","family":"Nakatani","sequence":"additional","affiliation":[{"name":"Department of Regulatory Bioorganic Chemistry, SANKEN (The Institute of Scientific and Industrial Research), Osaka University, 8-1 Mihogaoka, Ibaraki, Osaka 567-0047, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2021,8,6]]},"reference":[{"key":"2021090700081828100_B1","doi-asserted-by":"crossref","first-page":"777","DOI":"10.1016\/j.cell.2009.02.011","article-title":"RNA and disease","volume":"136","author":"Cooper","year":"2009","journal-title":"Cell"},{"key":"2021090700081828100_B2","doi-asserted-by":"crossref","first-page":"861","DOI":"10.1038\/nrg3074","article-title":"Non-coding RNAs in human disease","volume":"12","author":"Esteller","year":"2011","journal-title":"Nat. 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