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They function mainly by regulating interactions with specific RNA-binding proteins. Both modifications are linked to development, disease and stress response. To date, three methyltransferases and two demethylases have been identified that modify adenosines in mammalian mRNAs. Here, we present a comprehensive analysis of the interactomes of these enzymes. PCIF1 protein network comprises mostly factors involved in nascent RNA synthesis by RNA polymerase II, whereas ALKBH5 is closely linked with most aspects of pre-mRNA processing and mRNA export to the cytoplasm. METTL16 resides in subcellular compartments co-inhabited by several other RNA modifiers and processing factors. FTO interactome positions this demethylase at a crossroad between RNA transcription, RNA processing and DNA replication and repair. Altogether, these enzymes share limited spatial interactomes, pointing to specific molecular mechanisms of their regulation.<\/jats:p>","DOI":"10.1093\/nar\/gkab900","type":"journal-article","created":{"date-parts":[[2021,9,23]],"date-time":"2021-09-23T03:13:34Z","timestamp":1632366814000},"page":"10895-10910","source":"Crossref","is-referenced-by-count":50,"title":["The comprehensive interactomes of human adenosine RNA methyltransferases and demethylases reveal distinct functional and regulatory features"],"prefix":"10.1093","volume":"49","author":[{"given":"Helena","family":"Covelo-Molares","sequence":"first","affiliation":[{"name":"Central European Institute of Technology (CEITEC), Masaryk University, Brno 62500, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ales","family":"Obrdlik","sequence":"additional","affiliation":[{"name":"Central European Institute of Technology (CEITEC), Masaryk University, Brno 62500, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ivana","family":"Po\u0161tulkov\u00e1","sequence":"additional","affiliation":[{"name":"Central European Institute of Technology (CEITEC), Masaryk University, Brno 62500, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michaela","family":"Dohn\u00e1lkov\u00e1","sequence":"additional","affiliation":[{"name":"Central European Institute of Technology (CEITEC), Masaryk University, Brno 62500, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pavl\u00edna","family":"Gregorov\u00e1","sequence":"additional","affiliation":[{"name":"Central European Institute of Technology (CEITEC), Masaryk University, Brno 62500, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ranjani","family":"Ganji","sequence":"additional","affiliation":[{"name":"Central European Institute of Technology (CEITEC), Masaryk University, Brno 62500, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"Pot\u011b\u0161il","sequence":"additional","affiliation":[{"name":"Central European Institute of Technology (CEITEC), Masaryk University, Brno 62500, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lisa","family":"Gawriyski","sequence":"additional","affiliation":[{"name":"Institute of Biotechnology & HiLIFE \u2013 Helsinki Institute of Life Science, University of Helsinki, Helsinki 00014, Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Markku","family":"Varjosalo","sequence":"additional","affiliation":[{"name":"Institute of Biotechnology & HiLIFE \u2013 Helsinki Institute of Life Science, University of Helsinki, Helsinki 00014, Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8807-0684","authenticated-orcid":false,"given":"\u0160t\u011bp\u00e1nka","family":"Va\u0148\u00e1\u010dov\u00e1","sequence":"additional","affiliation":[{"name":"Central European Institute of Technology (CEITEC), Masaryk University, Brno 62500, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2021,10,11]]},"reference":[{"key":"2021110312485684100_B1","doi-asserted-by":"crossref","first-page":"201","DOI":"10.1038\/nature11112","article-title":"Topology of the human and mouse m6A RNA methylomes revealed by m6A-seq","volume":"485","author":"Dominissini","year":"2012","journal-title":"Nature"},{"key":"2021110312485684100_B2","doi-asserted-by":"crossref","first-page":"1635","DOI":"10.1016\/j.cell.2012.05.003","article-title":"Comprehensive analysis of mRNA methylation reveals enrichment in 3\u2032 UTRs and near stop codons","volume":"149","author":"Meyer","year":"2012","journal-title":"Cell"},{"key":"2021110312485684100_B3","doi-asserted-by":"crossref","first-page":"e1489","DOI":"10.1002\/wrna.1489","article-title":"RNA methylation in nuclear pre-mRNA processing","volume":"9","author":"Covelo-Molares","year":"2018","journal-title":"Wiley Interdiscip. 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