{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T02:31:37Z","timestamp":1790908297986,"version":"4.1.0"},"reference-count":66,"publisher":"Oxford University Press (OUP)","issue":"3","license":[{"start":{"date-parts":[[2022,1,31]],"date-time":"2022-01-31T00:00:00Z","timestamp":1643587200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Kimmelman\u00a0Center for Macromolecular Assemblies"},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["AI119788"],"award-info":[{"award-number":["AI119788"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Kimmelman Center for Structural Biology"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2022,2,22]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Giardiasis is a disease caused by the protist Giardia lamblia. As no human vaccines have been approved so far against it, and resistance to current drugs is spreading, new strategies for combating giardiasis need to be developed. The G. lamblia ribosome may provide a promising therapeutic target due to its distinct sequence differences from ribosomes of most eukaryotes and prokaryotes. Here, we report the cryo-electron microscopy structure of the G. lamblia (WB strain) ribosome determined at 2.75 \u00c5 resolution. The ribosomal RNA is the shortest known among eukaryotes, and lacks nearly all the eukaryote-specific ribosomal RNA expansion segments. In contrast, the ribosomal proteins are typically eukaryotic with some species-specific insertions\/extensions. Most typical inter-subunit bridges are maintained except for one missing contact site. Unique structural features are located mainly at the ribosome\u2019s periphery. These may be exploited as target sites for the design of new compounds that inhibit selectively the parasite\u2019s ribosomal activity.<\/jats:p>","DOI":"10.1093\/nar\/gkac046","type":"journal-article","created":{"date-parts":[[2022,1,25]],"date-time":"2022-01-25T12:22:30Z","timestamp":1643113350000},"page":"1770-1782","source":"Crossref","is-referenced-by-count":20,"title":["Cryo-EM structure of the ancient eukaryotic ribosome from the human parasite <i>Giardia lamblia<\/i>"],"prefix":"10.1093","volume":"50","author":[{"given":"Disha-Gajanan","family":"Hiregange","sequence":"first","affiliation":[{"name":"Department of Chemical and Structural Biology, Weizmann Institute of Science, Rehovot 7610001, Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andre","family":"Rivalta","sequence":"additional","affiliation":[{"name":"Department of Chemical and Structural Biology, Weizmann Institute of Science, Rehovot 7610001, Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tanaya","family":"Bose","sequence":"additional","affiliation":[{"name":"Department of Chemical and Structural Biology, Weizmann Institute of Science, Rehovot 7610001, Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elinor","family":"Breiner-Goldstein","sequence":"additional","affiliation":[{"name":"Department of Chemical and Structural Biology, Weizmann Institute of Science, Rehovot 7610001, Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sarit","family":"Samiya","sequence":"additional","affiliation":[{"name":"Department of Chemical and Structural Biology, Weizmann Institute of Science, Rehovot 7610001, Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giuseppe","family":"Cimicata","sequence":"additional","affiliation":[{"name":"Department of Chemical and Structural Biology, Weizmann Institute of Science, Rehovot 7610001, Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liudmila","family":"Kulakova","sequence":"additional","affiliation":[{"name":"Institute for Bioscience and Biotechnology Research, University of Maryland, Rockville, MD 20742-4454, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ella","family":"Zimmerman","sequence":"additional","affiliation":[{"name":"Department of Chemical and Structural Biology, Weizmann Institute of Science, Rehovot 7610001, Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7705-0466","authenticated-orcid":false,"given":"Anat","family":"Bashan","sequence":"additional","affiliation":[{"name":"Department of Chemical and Structural Biology, Weizmann Institute of Science, Rehovot 7610001, Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Osnat","family":"Herzberg","sequence":"additional","affiliation":[{"name":"Institute for Bioscience and Biotechnology Research, University of Maryland, Rockville, MD 20742-4454, USA"},{"name":"Department of Chemistry and Biochemistry, University of Maryland, College Park, MD 20742-4454, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ada","family":"Yonath","sequence":"additional","affiliation":[{"name":"Department of Chemical and Structural Biology, Weizmann Institute of Science, Rehovot 7610001, Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2022,1,31]]},"reference":[{"key":"2022022200024866400_B1","doi-asserted-by":"crossref","first-page":"24","DOI":"10.3390\/antibiotics5030024","article-title":"Ribosomal antibiotics: contemporary challenges","volume":"5","author":"Auerbach-Nevo","year":"2016","journal-title":"Antibiotics"},{"key":"2022022200024866400_B2","doi-asserted-by":"crossref","first-page":"274","DOI":"10.1016\/j.sbi.2011.01.007","article-title":"The ribosomal tunnel as a functional environment for nascent polypeptide folding and translational stalling","volume":"21","author":"Wilson","year":"2011","journal-title":"Curr. 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