{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T03:06:17Z","timestamp":1792033577776,"version":"4.4.0"},"reference-count":105,"publisher":"Oxford University Press (OUP)","issue":"7","license":[{"start":{"date-parts":[[2022,3,2]],"date-time":"2022-03-02T00:00:00Z","timestamp":1646179200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000268","name":"Biotechnology and Biological Sciences Research Council","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000268","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000265","name":"Medical Research Council","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000265","id-type":"DOI","asserted-by":"publisher"}]},{"name":"University of Sheffield Harry Worthington Scholarship"},{"DOI":"10.13039\/100009122","name":"Ministry of Higher Education and Scientific Research","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100009122","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2022,4,22]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Burkholderia cenocepacia is an opportunistic pathogen that causes severe infections of the cystic fibrosis (CF) lung. To acquire iron, B.\u00a0cenocepacia secretes the Fe(III)-binding compound, ornibactin. Genes for synthesis and utilisation of ornibactin are served by the iron starvation (IS) extracytoplasmic function (ECF) \u03c3 factor, OrbS. Transcription of orbS is regulated in response to the prevailing iron concentration by the ferric uptake regulator (Fur), such that orbS expression is repressed under iron-sufficient conditions. Here we show that, in addition to Fur-mediated regulation of orbS, the OrbS protein itself responds to intracellular iron availability. Substitution of cysteine residues in the C-terminal region of OrbS diminished the ability to respond to Fe(II) in vivo. Accordingly, whilst Fe(II) impaired transcription from and recognition of OrbS-dependent promoters in vitro by inhibiting the binding of OrbS to core RNA polymerase (RNAP), the cysteine-substituted OrbS variant was less responsive to Fe(II). Thus, the cysteine residues within the C-terminal region of OrbS contribute to an iron-sensing motif that serves as an on-board \u2018anti-\u03c3 factor\u2019 in the presence of Fe(II). A model to account for the presence two regulators (Fur and OrbS) that respond to the same intracellular Fe(II) signal to control ornibactin synthesis and utilisation is discussed.<\/jats:p>","DOI":"10.1093\/nar\/gkac137","type":"journal-article","created":{"date-parts":[[2022,2,14]],"date-time":"2022-02-14T20:25:48Z","timestamp":1644870348000},"page":"3709-3726","source":"Crossref","is-referenced-by-count":8,"title":["The <i>Burkholderia cenocepacia<\/i> iron starvation \u03c3 factor, OrbS, possesses an on-board iron sensor"],"prefix":"10.1093","volume":"50","author":[{"given":"Aaron T","family":"Butt","sequence":"first","affiliation":[{"name":"Department of Infection, Immunity and Cardiovascular Disease, Faculty of Medicine, Dentistry and Health, University of Sheffield, Sheffield S10 2RX, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christopher D","family":"Banyard","sequence":"additional","affiliation":[{"name":"Department of Infection, Immunity and Cardiovascular Disease, Faculty of Medicine, Dentistry and Health, University of Sheffield, Sheffield S10 2RX, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sayali S","family":"Haldipurkar","sequence":"additional","affiliation":[{"name":"Department of Infection, Immunity and Cardiovascular Disease, Faculty of Medicine, Dentistry and Health, University of Sheffield, Sheffield S10 2RX, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kirsty","family":"Agnoli","sequence":"additional","affiliation":[{"name":"Department of Infection, Immunity and Cardiovascular Disease, Faculty of Medicine, Dentistry and Health, University of Sheffield, Sheffield S10 2RX, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Muslim\u00a0I","family":"Mohsin","sequence":"additional","affiliation":[{"name":"Department of Infection, Immunity and Cardiovascular Disease, Faculty of Medicine, Dentistry and Health, University of Sheffield, Sheffield S10 2RX, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Srdjan","family":"Vitovski","sequence":"additional","affiliation":[{"name":"Department of Infection, Immunity and Cardiovascular Disease, Faculty of Medicine, Dentistry and Health, University of Sheffield, Sheffield S10 2RX, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ameya","family":"Paleja","sequence":"additional","affiliation":[{"name":"Department of Infection, Immunity and Cardiovascular Disease, Faculty of Medicine, Dentistry and Health, University of Sheffield, Sheffield S10 2RX, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yingzhi","family":"Tang","sequence":"additional","affiliation":[{"name":"Department of Infection, Immunity and Cardiovascular Disease, Faculty of Medicine, Dentistry and Health, University of Sheffield, Sheffield S10 2RX, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rebecca","family":"Lomax","sequence":"additional","affiliation":[{"name":"Department of Infection, Immunity and Cardiovascular Disease, Faculty of Medicine, Dentistry and Health, University of Sheffield, Sheffield S10 2RX, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fuzhou","family":"Ye","sequence":"additional","affiliation":[{"name":"Section of Structural Biology, Department of Infectious Disease, Imperial College London, Sir Alexander Fleming Building, South Kensington, London\u00a0SW7 2AZ, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2238-6464","authenticated-orcid":false,"given":"Jeffrey","family":"Green","sequence":"additional","affiliation":[{"name":"School of Biosciences, University of Sheffield, Sheffield S10 2TN, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0701-2584","authenticated-orcid":false,"given":"Mark\u00a0S","family":"Thomas","sequence":"additional","affiliation":[{"name":"Department of Infection, Immunity and Cardiovascular Disease, Faculty of Medicine, Dentistry and Health, University of Sheffield, Sheffield S10 2RX, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2022,3,2]]},"reference":[{"key":"2022042200034838700_B1","doi-asserted-by":"crossref","first-page":"848","DOI":"10.3390\/biom5020848","article-title":"Structural biology of bacterial RNA polymerase","volume":"5","author":"Murakami","year":"2015","journal-title":"Biomolecules"},{"key":"2022042200034838700_B2","doi-asserted-by":"crossref","first-page":"357","DOI":"10.1146\/annurev-micro-092412-155737","article-title":"Bacterial sigma factors: a historical, structural, and genomic perspective","volume":"68","author":"Fekl\u00edstov","year":"2014","journal-title":"Annu. 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