{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,17]],"date-time":"2026-06-17T01:49:15Z","timestamp":1781660955521,"version":"3.54.5"},"reference-count":52,"publisher":"Oxford University Press (OUP)","issue":"11","license":[{"start":{"date-parts":[[2022,6,3]],"date-time":"2022-06-03T00:00:00Z","timestamp":1654214400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/100010663","name":"European Research Council","doi-asserted-by":"publisher","award":["FP7\/2007-2013\/28159"],"award-info":[{"award-number":["FP7\/2007-2013\/28159"]}],"id":[{"id":"10.13039\/100010663","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Agence Nationale pour la Recherche","award":["2016-CE12-0030-0"],"award-info":[{"award-number":["2016-CE12-0030-0"]}]},{"name":"Agence Nationale pour la Recherche","award":["2021-CE35-0013-01"],"award-info":[{"award-number":["2021-CE35-0013-01"]}]},{"name":"Agence Nationale pour la Recherche","award":["2018-CE12-0012-03"],"award-info":[{"award-number":["2018-CE12-0012-03"]}]},{"DOI":"10.13039\/501100002915","name":"Fondation pour la Recherche M\u00e9dicale","doi-asserted-by":"publisher","award":["EQU202003010328"],"award-info":[{"award-number":["EQU202003010328"]}],"id":[{"id":"10.13039\/501100002915","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2022,6,24]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>The chromosome dimer resolution machinery of bacteria is generally composed of two tyrosine recombinases, XerC and XerD. They resolve chromosome dimers by adding a crossover between sister copies of a specific site, dif. The reaction depends on a cell division protein, FtsK, which activates XerD by protein-protein interactions. The toxin-linked cryptic satellite phage (TLC\u03a6) of Vibrio cholerae, which participates in the emergence of cholera epidemic strains, carries a dif-like attachment site (attP). TLC\u03a6 exploits the Xer machinery to integrate into the dif site of its host chromosomes. The TLC\u03a6 integration reaction escapes the control of FtsK because TLC\u03a6 encodes for its own XerD-activation factor, XafT. Additionally, TLC\u03a6 attP is a poor substrate for XerD binding, in apparent contradiction with the high integration efficiency of the phage. Here, we present a sequencing-based methodology to analyse the integration and excision efficiency of thousands of synthetic mini-TLC\u03a6 plasmids with differing attP sites in vivo. This methodology is applicable to the fine-grained analyses of DNA transactions on a wider scale. In addition, we compared the efficiency with which XafT and the XerD-activation domain of FtsK drive recombination reactions in vitro. Our results suggest that XafT not only activates XerD-catalysis but also helps form and\/or stabilize synaptic complexes between imperfect Xer recombination sites.<\/jats:p>","DOI":"10.1093\/nar\/gkac429","type":"journal-article","created":{"date-parts":[[2022,6,3]],"date-time":"2022-06-03T08:22:25Z","timestamp":1654244545000},"page":"6368-6383","source":"Crossref","is-referenced-by-count":13,"title":["The Xer activation factor of TLC\u03a6 expands the possibilities for Xer recombination"],"prefix":"10.1093","volume":"50","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3189-1881","authenticated-orcid":false,"given":"Solange","family":"Miele","sequence":"first","affiliation":[{"name":"Institute for Integrative Biology of the Cell (I2BC), Universit\u00e9 Paris-Saclay, CEA, CNRS , 1 Avenue de la Terrasse , 91198\u00a0Gif-sur-Yvette , France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7223-8411","authenticated-orcid":false,"given":"James Iain","family":"Provan","sequence":"additional","affiliation":[{"name":"Institute for Integrative Biology of the Cell (I2BC), Universit\u00e9 Paris-Saclay, CEA, CNRS , 1 Avenue de la Terrasse , 91198\u00a0Gif-sur-Yvette , France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Justine","family":"Vergne","sequence":"additional","affiliation":[{"name":"Institute for Integrative Biology of the Cell (I2BC), Universit\u00e9 Paris-Saclay, CEA, CNRS , 1 Avenue de la Terrasse , 91198\u00a0Gif-sur-Yvette , France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christophe","family":"Possoz","sequence":"additional","affiliation":[{"name":"Institute for Integrative Biology of the Cell (I2BC), Universit\u00e9 Paris-Saclay, CEA, CNRS , 1 Avenue de la Terrasse , 91198\u00a0Gif-sur-Yvette , France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fran\u00e7oise","family":"Ochsenbein","sequence":"additional","affiliation":[{"name":"Institute for Integrative Biology of the Cell (I2BC), Universit\u00e9 Paris-Saclay, CEA, CNRS , 1 Avenue de la Terrasse , 91198\u00a0Gif-sur-Yvette , France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9170-9896","authenticated-orcid":false,"given":"Fran\u00e7ois-Xavier","family":"Barre","sequence":"additional","affiliation":[{"name":"Institute for Integrative Biology of the Cell (I2BC), Universit\u00e9 Paris-Saclay, CEA, CNRS , 1 Avenue de la Terrasse , 91198\u00a0Gif-sur-Yvette , France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2022,6,3]]},"reference":[{"key":"2022062405241275200_B1","doi-asserted-by":"crossref","DOI":"10.1128\/microbiolspec.MDNA3-0056-2014","article-title":"Xer site-specific recombination: promoting vertical and horizontal transmission of genetic information","volume":"2","author":"Midonet","year":"2014","journal-title":"Microbiol. Spectr."},{"key":"2022062405241275200_B2","doi-asserted-by":"crossref","first-page":"351","DOI":"10.1016\/0092-8674(93)80076-Q","article-title":"Two related recombinases are required for site-specific recombination at dif and cer in e. coli K12","volume":"75","author":"Blakely","year":"1993","journal-title":"Cell"},{"key":"2022062405241275200_B3","doi-asserted-by":"crossref","first-page":"6973","DOI":"10.1128\/jb.172.12.6973-6980.1990","article-title":"Recombination at cole1 cer requires the Escherichia coli xerC gene product, a member of the lambda integrase family of site-specific recombinases","volume":"172","author":"Colloms","year":"1990","journal-title":"J. Bacteriol."},{"key":"2022062405241275200_B4","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1016\/j.tim.2012.10.003","article-title":"Integrative mobile elements exploiting xer recombination","volume":"21","author":"Das","year":"2013","journal-title":"Trends Microbiol."},{"key":"2022062405241275200_B5","doi-asserted-by":"crossref","first-page":"699","DOI":"10.1111\/mmi.14563","article-title":"Mobilization of pdif modules in acinetobacter: a novel mechanism for antibiotic resistance gene shuffling","volume":"114","author":"Balalovski","year":"2020","journal-title":"Mol. Microbiol."},{"key":"2022062405241275200_B6","doi-asserted-by":"crossref","first-page":"656","DOI":"10.1038\/nature00782","article-title":"Filamentous phage integration requires the host recombinases XerC and xerD","volume":"417","author":"Huber","year":"2002","journal-title":"Nature"},{"key":"2022062405241275200_B7","doi-asserted-by":"crossref","first-page":"4377","DOI":"10.1073\/pnas.0910212107","article-title":"Molecular keys of the tropism of integration of the cholera toxin phage","volume":"107","author":"Das","year":"2010","journal-title":"Proc. Natl. Acad. Sci. U.S.A."},{"key":"2022062405241275200_B8","doi-asserted-by":"crossref","first-page":"2516","DOI":"10.1073\/pnas.1017061108","article-title":"VGJphi integration and excision mechanisms contribute to the genetic diversity of vibrio cholerae epidemic strains","volume":"108","author":"Das","year":"2011","journal-title":"Proc. Natl. Acad. Sci. 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