{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T20:30:43Z","timestamp":1790713843277,"version":"4.1.0"},"reference-count":103,"publisher":"Oxford University Press (OUP)","issue":"11","license":[{"start":{"date-parts":[[2022,6,3]],"date-time":"2022-06-03T00:00:00Z","timestamp":1654214400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Russian Ministry of Science and Higher Education","award":["075-15-2021-1062"],"award-info":[{"award-number":["075-15-2021-1062"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2022,6,24]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>The X family polymerases (PolXs) are specialized DNA polymerases that are found in all domains of life. While the main representatives of eukaryotic PolXs, which have dedicated functions in DNA repair, were studied in much detail, the functions and diversity of prokaryotic PolXs have remained largely unexplored. Here, by combining a comprehensive bioinformatic analysis of prokaryotic PolXs and biochemical experiments involving selected recombinant enzymes, we reveal a previously unrecognized group of PolXs that seem to be lacking DNA polymerase activity. The noncanonical PolXs contain substitutions of the key catalytic residues and deletions in their polymerase and dNTP binding sites in the palm and fingers domains, but contain functional nuclease domains, similar to canonical PolXs. We demonstrate that representative noncanonical PolXs from the Deinococcus genus are indeed inactive as DNA polymerases but are highly efficient as 3\u2032-5\u2032 exonucleases. We show that both canonical and noncanonical PolXs are often encoded together with the components of the non-homologous end joining pathway and may therefore participate in double-strand break repair, suggesting an evolutionary conservation of this PolX function. This is a remarkable example of polymerases that have lost their main polymerase activity, but retain accessory functions in DNA processing and repair.<\/jats:p>","DOI":"10.1093\/nar\/gkac461","type":"journal-article","created":{"date-parts":[[2022,6,3]],"date-time":"2022-06-03T12:23:18Z","timestamp":1654258998000},"page":"6398-6413","source":"Crossref","is-referenced-by-count":10,"title":["Noncanonical prokaryotic X family DNA polymerases lack polymerase activity and act as exonucleases"],"prefix":"10.1093","volume":"50","author":[{"given":"Maria","family":"Prostova","sequence":"first","affiliation":[{"name":"Institute of Molecular Genetics, National Research Centre \u201cKurchatov Institute\u201d , Moscow 123182 , Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Evgeniy","family":"Shilkin","sequence":"additional","affiliation":[{"name":"Institute of Molecular Genetics, National Research Centre \u201cKurchatov Institute\u201d , Moscow 123182 , Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexandra A","family":"Kulikova","sequence":"additional","affiliation":[{"name":"Institute of Molecular Genetics, National Research Centre \u201cKurchatov Institute\u201d , Moscow 123182 , Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alena","family":"Makarova","sequence":"additional","affiliation":[{"name":"Institute of Molecular Genetics, National Research Centre \u201cKurchatov Institute\u201d , Moscow 123182 , Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3171-2997","authenticated-orcid":false,"given":"Sergei","family":"Ryazansky","sequence":"additional","affiliation":[{"name":"Institute of Molecular Genetics, National Research Centre \u201cKurchatov Institute\u201d , Moscow 123182 , Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2292-6424","authenticated-orcid":false,"given":"Andrey","family":"Kulbachinskiy","sequence":"additional","affiliation":[{"name":"Institute of Molecular Genetics, National Research Centre \u201cKurchatov Institute\u201d , Moscow 123182 , Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2022,6,3]]},"reference":[{"key":"2022062405251201000_B1","doi-asserted-by":"crossref","first-page":"239","DOI":"10.1042\/BST20180579","article-title":"An updated structural classification of replicative DNA polymerases","volume":"47","author":"Raia","year":"2019","journal-title":"Biochem. 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