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This challenge represents a major roadblock to genome engineering applications. Here we exploit the unmatched heterologous DNA cargo capacity of baculovirus to resolve this bottleneck in human cells. By encoding Cas9, sgRNA and Donor DNAs on a single, rapidly assembled baculoviral vector, we achieve with up to 30% efficacy whole-exon replacement in the intronic \u03b2-actin (ACTB) locus, including site-specific docking of very large DNA payloads. We use our approach to rescue wild-type podocin expression in steroid-resistant nephrotic syndrome (SRNS) patient derived podocytes. We demonstrate single baculovirus vectored delivery of single and multiplexed prime-editing toolkits, achieving up to 100% cleavage-free DNA search-and-replace interventions without detectable indels. Taken together, we provide a versatile delivery platform for single base to multi-gene level genome interventions, addressing the currently unmet need for a powerful delivery system accommodating current and future CRISPR technologies without the burden of limited cargo capacity.<\/jats:p>","DOI":"10.1093\/nar\/gkac587","type":"journal-article","created":{"date-parts":[[2022,7,8]],"date-time":"2022-07-08T09:16:20Z","timestamp":1657271780000},"page":"7783-7799","source":"Crossref","is-referenced-by-count":46,"title":["Highly efficient CRISPR-mediated large DNA docking and multiplexed prime editing using a single baculovirus"],"prefix":"10.1093","volume":"50","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3097-3765","authenticated-orcid":false,"given":"Francesco","family":"Aulicino","sequence":"first","affiliation":[{"name":"BrisSynBio Bristol Synthetic Biology Centre, Biomedical Sciences, School of Biochemistry, 1 Tankard's Close, University of Bristol , Bristol BS8 1TD, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martin","family":"Pelosse","sequence":"additional","affiliation":[{"name":"BrisSynBio Bristol Synthetic Biology Centre, Biomedical Sciences, School of Biochemistry, 1 Tankard's Close, University of Bristol , Bristol BS8 1TD, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9984-5940","authenticated-orcid":false,"given":"Christine","family":"Toelzer","sequence":"additional","affiliation":[{"name":"BrisSynBio Bristol Synthetic Biology Centre, Biomedical Sciences, School of Biochemistry, 1 Tankard's Close, University of Bristol , Bristol BS8 1TD, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julien","family":"Capin","sequence":"additional","affiliation":[{"name":"BrisSynBio Bristol Synthetic Biology Centre, Biomedical Sciences, School of Biochemistry, 1 Tankard's Close, University of Bristol , Bristol BS8 1TD, 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Bristol , Bristol BS8 1TD, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Moin\u00a0A","family":"Saleem","sequence":"additional","affiliation":[{"name":"Bristol Renal, Bristol Medical School, Dorothy Hodgkin Building , Whitson street, Bristol BS1 3NY, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gavin\u00a0I","family":"Welsh","sequence":"additional","affiliation":[{"name":"Bristol Renal, Bristol Medical School, Dorothy Hodgkin Building , Whitson street, Bristol BS1 3NY, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Imre","family":"Berger","sequence":"additional","affiliation":[{"name":"BrisSynBio Bristol Synthetic Biology Centre, Biomedical Sciences, School of Biochemistry, 1 Tankard's Close, University of Bristol , Bristol BS8 1TD, UK"},{"name":"Max Planck Bristol Centre for Minimal Biology, School of Chemistry, University of Bristol , Cantock's Close, Bristol BS8 1TS, 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Biotechnol."},{"key":"2022072200030743300_B73","doi-asserted-by":"crossref","first-page":"759","DOI":"10.3390\/pharmaceutics12080759","article-title":"Synthetic virus-derived nanosystems (SVNs) for delivery and precision docking of large multifunctional DNA circuitry in mammalian cells","volume":"12","author":"Aulicino","year":"2020","journal-title":"Pharmaceutics"},{"key":"2022072200030743300_B74","doi-asserted-by":"crossref","first-page":"871","DOI":"10.1089\/104303401750195845","article-title":"In vivo gene transfer in mouse skeletal muscle mediated by baculovirus vectors","volume":"12","author":"Pieroni","year":"2001","journal-title":"Hum. Gene Ther."},{"key":"2022072200030743300_B75","doi-asserted-by":"crossref","first-page":"2011","DOI":"10.1089\/hum.1997.8.17-2011","article-title":"Efficient transduction of mammalian cells by a recombinant baculovirus having the vesicular stomatitis virus G glycoprotein","volume":"8","author":"Barsoum","year":"1997","journal-title":"Hum. Gene Ther."},{"key":"2022072200030743300_B76","doi-asserted-by":"crossref","first-page":"126","DOI":"10.1038\/s41551-018-0318-7","article-title":"Spatial control of in vivo CRISPR-Cas9 genome editing via nanomagnets","volume":"3","author":"Zhu","year":"2019","journal-title":"Nat. Biomed. Eng."},{"key":"2022072200030743300_B77","doi-asserted-by":"crossref","first-page":"451","DOI":"10.1038\/88122","article-title":"Incorporation of decay-accelerating factor into the baculovirus envelope generates complement-resistant gene transfer vectors","volume":"19","author":"Huser","year":"2001","journal-title":"Nat. Biotechnol."},{"key":"2022072200030743300_B78","doi-asserted-by":"crossref","first-page":"987","DOI":"10.1038\/mt.2010.25","article-title":"Screening of complement inhibitors: shielded baculoviruses increase the safety and efficacy of gene delivery","volume":"18","author":"Kaikkonen","year":"2010","journal-title":"Mol. Ther."},{"key":"2022072200030743300_B79","doi-asserted-by":"crossref","first-page":"127","DOI":"10.1073\/pnas.96.1.127","article-title":"Transient and stable gene expression in mammalian cells transduced with a recombinant baculovirus vector","volume":"96","author":"Condreay","year":"1999","journal-title":"Proc. Nat. Acad. Sci. U.S.A."},{"key":"2022072200030743300_B80","doi-asserted-by":"crossref","first-page":"373","DOI":"10.1021\/bp025609d","article-title":"Enhancement and prolongation of baculovirus-mediated expression in mammalian cells: focuses on strategic infection and feeding","volume":"19","author":"Hu","year":"2003","journal-title":"Biotechnol. Prog."},{"key":"2022072200030743300_B81","doi-asserted-by":"crossref","first-page":"194","DOI":"10.1016\/j.omtm.2017.07.002","article-title":"Global screening of antiviral genes that suppress baculovirus transgene expression in mammalian cells","volume":"6","author":"Wang","year":"2017","journal-title":"Mol. Ther. Methods Clin. 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