{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T20:24:57Z","timestamp":1787775897911,"version":"build-2784847793"},"reference-count":87,"publisher":"Oxford University Press (OUP)","issue":"15","license":[{"start":{"date-parts":[[2022,7,29]],"date-time":"2022-07-29T00:00:00Z","timestamp":1659052800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000002","name":"NIH","doi-asserted-by":"publisher","award":["R01AI150448"],"award-info":[{"award-number":["R01AI150448"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"NIH","doi-asserted-by":"publisher","award":["R01DE025447"],"award-info":[{"award-number":["R01DE025447"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"NIH","doi-asserted-by":"publisher","award":["R56AI157872"],"award-info":[{"award-number":["R56AI157872"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"NIH","doi-asserted-by":"publisher","award":["R01CA260690"],"award-info":[{"award-number":["R01CA260690"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"NIH","doi-asserted-by":"publisher","award":["R03DE029716"],"award-info":[{"award-number":["R03DE029716"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100005166","name":"OSU","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100005166","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2022,8,26]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>FACT (FAcilitates Chromatin Transcription) is a heterodimeric protein complex composed of SUPT16H and SSRP1, and a histone chaperone participating in chromatin remodeling during gene transcription. FACT complex is profoundly regulated, and contributes to both gene activation and suppression. Here we reported that SUPT16H, a subunit of FACT, is acetylated in both epithelial and natural killer (NK) cells. The histone acetyltransferase TIP60 contributes to the acetylation of SUPT16H middle domain (MD) at lysine 674 (K674). Such acetylation of SUPT16H is recognized by bromodomain protein BRD4, which promotes protein stability of SUPT16H in both epithelial and NK cells. We further demonstrated that SUPT16H-BRD4 associates with histone modification enzymes (HDAC1, EZH2), and further regulates their activation status and\/or promoter association as well as affects the relevant histone marks (H3ac, H3K9me3 and H3K27me3). BRD4 is known to profoundly regulate interferon (IFN) signaling, while such function of SUPT16H has never been explored. Surprisingly, our results revealed that SUPT16H genetic knockdown via RNAi or pharmacological inhibition by using its inhibitor, curaxin 137 (CBL0137), results in the induction of IFNs and interferon-stimulated genes (ISGs). Through this mechanism, depletion or inhibition of SUPT16H is shown to efficiently inhibit infection of multiple viruses, including Zika, influenza, and SARS-CoV-2. Furthermore, we demonstrated that depletion or inhibition of SUPT16H also causes the remarkable activation of IFN signaling in NK cells, which promotes the NK-mediated killing of virus-infected cells in a co-culture system using human primary NK cells. Overall, our studies unraveled the previously un-appreciated role of FACT complex in coordinating with BRD4 and regulating IFN signaling in both epithelial and NK cells, and also proposed the novel application of the FACT inhibitor CBL0137 to treat viral infections.<\/jats:p>","DOI":"10.1093\/nar\/gkac645","type":"journal-article","created":{"date-parts":[[2022,7,29]],"date-time":"2022-07-29T11:39:56Z","timestamp":1659094796000},"page":"8700-8718","source":"Crossref","is-referenced-by-count":22,"title":["FACT subunit SUPT16H associates with BRD4 and contributes to silencing of interferon signaling"],"prefix":"10.1093","volume":"50","author":[{"given":"Dawei","family":"Zhou","sequence":"first","affiliation":[{"name":"Department of Pathology, The Ohio State University Wexner Medical Center , Columbus , OH\u00a0 43210 ,\u00a0 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhenyu","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Pathology, The Ohio State University Wexner Medical Center , Columbus , OH\u00a0 43210 ,\u00a0 USA"},{"name":"Department of Biomedical Informatics, The Ohio State University Wexner Medical Center , Columbus , OH\u00a0 43210 ,\u00a0 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jun-Gyu","family":"Park","sequence":"additional","affiliation":[{"name":"Texas Biomedical Research Institute , San Antonio , TX \u00a078227,\u00a0 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guillaume N","family":"Fiches","sequence":"additional","affiliation":[{"name":"Department of Pathology, The Ohio State University Wexner Medical Center , Columbus , OH\u00a0 43210 ,\u00a0 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7364-0805","authenticated-orcid":false,"given":"Tai-Wei","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Pathology, The Ohio State University Wexner Medical Center , Columbus , OH\u00a0 43210 ,\u00a0 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qin","family":"Ma","sequence":"additional","affiliation":[{"name":"Department of Biomedical Informatics, The Ohio State University Wexner Medical Center , Columbus , OH\u00a0 43210 ,\u00a0 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huachao","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Medicine, Columbia University Medical Center , NY , NY \u00a010032,\u00a0 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ayan","family":"Biswas","sequence":"additional","affiliation":[{"name":"Department of Pathology, The Ohio State University Wexner Medical Center , Columbus , OH\u00a0 43210 ,\u00a0 USA"},{"name":"Department of Genetics, The University of Alabama at Birmingham , Birmingham , AL\u00a0 35233 ,\u00a0 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luis","family":"Martinez-Sobrido","sequence":"additional","affiliation":[{"name":"Texas Biomedical Research Institute , San Antonio , TX \u00a078227,\u00a0 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1175-5831","authenticated-orcid":false,"given":"Netty G","family":"Santoso","sequence":"additional","affiliation":[{"name":"Department of Pathology, The Ohio State University Wexner Medical Center , Columbus , OH\u00a0 43210 ,\u00a0 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9856-8320","authenticated-orcid":false,"given":"Jian","family":"Zhu","sequence":"additional","affiliation":[{"name":"Department of Pathology, The Ohio State University Wexner Medical Center , Columbus , OH\u00a0 43210 ,\u00a0 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2022,7,29]]},"reference":[{"key":"2022082600051784000_B1","doi-asserted-by":"crossref","first-page":"141","DOI":"10.1038\/nrm.2016.159","article-title":"Histone chaperone networks shaping chromatin function","volume":"18","author":"Hammond","year":"2017","journal-title":"Nat. Rev. Mol. Cell Biol."},{"key":"2022082600051784000_B2","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1016\/S0092-8674(00)80903-4","article-title":"FACT, a factor that facilitates transcript elongation through nucleosomes","volume":"92","author":"Orphanides","year":"1998","journal-title":"Cell"},{"key":"2022082600051784000_B3","doi-asserted-by":"crossref","first-page":"1090","DOI":"10.1126\/science.1085703","article-title":"FACT facilitates transcription-dependent nucleosome alteration","volume":"301","author":"Belotserkovskaya","year":"2003","journal-title":"Science"},{"key":"2022082600051784000_B4","doi-asserted-by":"crossref","first-page":"8884","DOI":"10.1073\/pnas.0712293105","article-title":"The FACT spt16 \u201cpeptidase\u201d domain is a histone H3-H4 binding module","volume":"105","author":"Stuwe","year":"2008","journal-title":"Proc. Natl. Acad. Sci. U.S.A."},{"key":"2022082600051784000_B5","doi-asserted-by":"crossref","first-page":"11929","DOI":"10.1093\/nar\/gkaa912","article-title":"The role of FACT in managing chromatin: disruption, assembly, or repair?","volume":"48","author":"Formosa","year":"2020","journal-title":"Nucleic Acids Res."},{"key":"2022082600051784000_B6","doi-asserted-by":"crossref","first-page":"736","DOI":"10.1016\/j.tibs.2016.07.010","article-title":"CENPs and sweet nucleosomes face the FACT","volume":"41","author":"Murawska","year":"2016","journal-title":"Trends Biochem. Sci."},{"key":"2022082600051784000_B7","doi-asserted-by":"crossref","first-page":"4011","DOI":"10.1093\/nar\/gkz055","article-title":"FACT subunit spt16 controls UVSSA recruitment to lesion-stalled RNA pol II and stimulates TC-NER","volume":"47","author":"Wienholz","year":"2019","journal-title":"Nucleic Acids Res."},{"key":"2022082600051784000_B8","doi-asserted-by":"crossref","first-page":"903","DOI":"10.1038\/mt.2016.9","article-title":"SSRP1 contributes to the malignancy of hepatocellular carcinoma and is negatively regulated by miR-497","volume":"24","author":"Ding","year":"2016","journal-title":"Mol. Ther."},{"key":"2022082600051784000_B9","doi-asserted-by":"crossref","first-page":"27297","DOI":"10.1074\/jbc.M115.652339","article-title":"FACT proteins, SUPT16H and SSRP1, are transcriptional suppressors of HIV-1 and HTLV-1 that facilitate viral latency","volume":"290","author":"Huang","year":"2015","journal-title":"J. Biol. Chem."},{"key":"2022082600051784000_B10","doi-asserted-by":"crossref","first-page":"653","DOI":"10.1016\/j.molcel.2020.03.025","article-title":"MYC controls the Epstein-Barr virus lytic switch","volume":"78","author":"Guo","year":"2020","journal-title":"Mol. Cell"},{"key":"2022082600051784000_B11","doi-asserted-by":"crossref","first-page":"1096","DOI":"10.1126\/science.1087374","article-title":"Transcription elongation factors repress transcription initiation from cryptic sites","volume":"301","author":"Kaplan","year":"2003","journal-title":"Science"},{"key":"2022082600051784000_B12","doi-asserted-by":"crossref","first-page":"501","DOI":"10.1016\/j.molcel.2019.11.016","article-title":"The chaperone FACT and histone H2B ubiquitination maintain s. pombe genome architecture through genic and subtelomeric functions","volume":"77","author":"Murawska","year":"2020","journal-title":"Mol. Cell"},{"key":"2022082600051784000_B13","doi-asserted-by":"crossref","first-page":"10211","DOI":"10.1093\/nar\/gkaa732","article-title":"Histone chaperone FACT represses retrotransposon MERVL and MERVL-derived cryptic promoters","volume":"48","author":"Chen","year":"2020","journal-title":"Nucleic Acids Res."},{"key":"2022082600051784000_B14","doi-asserted-by":"crossref","first-page":"1485","DOI":"10.1101\/gad.1887310","article-title":"Ubiquitylation of FACT by the cullin-E3 ligase rtt101 connects FACT to DNA replication","volume":"24","author":"Han","year":"2010","journal-title":"Genes Dev."},{"key":"2022082600051784000_B15","doi-asserted-by":"crossref","first-page":"834","DOI":"10.1126\/science.1175371","article-title":"Lysine acetylation targets protein complexes and co-regulates major cellular functions","volume":"325","author":"Choudhary","year":"2009","journal-title":"Science"},{"key":"2022082600051784000_B16","doi-asserted-by":"crossref","first-page":"212","DOI":"10.1016\/j.molcel.2012.01.026","article-title":"Proteomic investigations reveal a role for RNA processing factor THRAP3 in the DNA damage response","volume":"46","author":"Beli","year":"2012","journal-title":"Mol. Cell"},{"key":"2022082600051784000_B17","doi-asserted-by":"crossref","first-page":"634","DOI":"10.1038\/nmeth.2518","article-title":"Integrated proteomic analysis of post-translational modifications by serial enrichment","volume":"10","author":"Mertins","year":"2013","journal-title":"Nat. Methods"},{"key":"2022082600051784000_B18","doi-asserted-by":"crossref","first-page":"842","DOI":"10.1016\/j.celrep.2013.07.024","article-title":"Lysine succinylation is a frequently occurring modification in prokaryotes and eukaryotes and extensively overlaps with acetylation","volume":"4","author":"Weinert","year":"2013","journal-title":"Cell Rep."},{"key":"2022082600051784000_B19","doi-asserted-by":"crossref","first-page":"7333","DOI":"10.1093\/nar\/gkz493","article-title":"A CRISPR\/Cas9 screen identifies the histone demethylase MINA53 as a novel HIV-1 latency-promoting gene (LPG)","volume":"47","author":"Huang","year":"2019","journal-title":"Nucleic Acids Res."},{"key":"2022082600051784000_B20","doi-asserted-by":"crossref","first-page":"eaba1941","DOI":"10.1126\/sciadv.aba1941","article-title":"Inhibition of Polo-like kinase 1 (PLK1) facilitates the elimination of HIV-1 viral reservoirs in CD4(+) t cells ex vivo","volume":"6","author":"Zhou","year":"2020","journal-title":"Sci. Adv."},{"key":"2022082600051784000_B21","doi-asserted-by":"crossref","first-page":"e1009764","DOI":"10.1371\/journal.ppat.1009764","article-title":"Inhibition of polo-like kinase 1 (PLK1) facilitates reactivation of gamma-herpesviruses and their elimination","volume":"17","author":"Biswas","year":"2021","journal-title":"PLoS Pathog."},{"key":"2022082600051784000_B22","doi-asserted-by":"crossref","first-page":"e1010503","DOI":"10.1371\/journal.ppat.1010503","article-title":"Polyamine biosynthesis and eIF5A hypusination are modulated by the DNA tumor virus KSHV and promote KSHV viral infection","volume":"18","author":"Fiches","year":"2022","journal-title":"PLoS Pathog."},{"key":"2022082600051784000_B23","doi-asserted-by":"crossref","first-page":"550","DOI":"10.1186\/s13059-014-0550-8","article-title":"Moderated estimation of fold change and dispersion for RNA-seq data with DESeq2","volume":"15","author":"Love","year":"2014","journal-title":"Genome Biol."},{"key":"2022082600051784000_B24","doi-asserted-by":"crossref","first-page":"1902","DOI":"10.1002\/ijc.32582","article-title":"Potent antileukemic activity of curaxin CBL0137 against MLL-rearranged leukemia","volume":"146","author":"Somers","year":"2020","journal-title":"Int. J. Cancer"},{"key":"2022082600051784000_B25","doi-asserted-by":"crossref","first-page":"404","DOI":"10.1186\/s12859-016-1274-4","article-title":"ChiLin: a comprehensive chip-seq and DNase-seq quality control and analysis pipeline","volume":"17","author":"Qin","year":"2016","journal-title":"BMC Bioinformatics"},{"key":"2022082600051784000_B26","doi-asserted-by":"crossref","first-page":"13353","DOI":"10.1073\/pnas.1103105108","article-title":"Cooperative transcription factor associations discovered using regulatory variation","volume":"108","author":"Karczewski","year":"2011","journal-title":"Proc. Natl. Acad. Sci. U.S.A."},{"key":"2022082600051784000_B27","first-page":"1948","article-title":"ChIP-seq analysis of genomic binding regions of five major transcription factors highlights a central role for ZIC2 in the mouse epiblast stem cell gene regulatory network","volume":"144","author":"Matsuda","year":"2017","journal-title":"Development"},{"key":"2022082600051784000_B28","doi-asserted-by":"crossref","first-page":"2020","DOI":"10.1073\/pnas.1911708117","article-title":"CTCF mediates chromatin looping via N-terminal domain-dependent cohesin retention","volume":"117","author":"Pugacheva","year":"2020","journal-title":"Proc. Natl. Acad. Sci. U.S.A."},{"key":"2022082600051784000_B29","doi-asserted-by":"crossref","first-page":"e00235\u201318","DOI":"10.1128\/JVI.00235-18","article-title":"Interleukin-15-stimulated natural killer cells clear HIV-1-Infected cells following latency reversal ex vivo","volume":"92","author":"Garrido","year":"2018","journal-title":"J. Virol."},{"key":"2022082600051784000_B30","doi-asserted-by":"crossref","first-page":"113995","DOI":"10.1016\/j.jviromet.2020.113995","article-title":"Rapid in vitro assays for screening neutralizing antibodies and antivirals against SARS-CoV-2","volume":"287","author":"Park","year":"2021","journal-title":"J. Virol. Methods"},{"key":"2022082600051784000_B31","doi-asserted-by":"crossref","first-page":"1196","DOI":"10.1101\/gad.14.10.1196","article-title":"The something about silencing protein, sas3, is the catalytic subunit of nua3, a yTAF(II)30-containing HAT complex that interacts with the spt16 subunit of the yeast CP (Cdc68\/Pob3)-FACT complex","volume":"14","author":"John","year":"2000","journal-title":"Genes Dev."},{"key":"2022082600051784000_B32","doi-asserted-by":"crossref","first-page":"9456","DOI":"10.1093\/nar\/gky659","article-title":"TIP60 represses activation of endogenous retroviral elements","volume":"46","author":"Rajagopalan","year":"2018","journal-title":"Nucleic Acids Res."},{"key":"2022082600051784000_B33","doi-asserted-by":"crossref","first-page":"e1006272","DOI":"10.1371\/journal.pgen.1006272","article-title":"Acetylation reader proteins: linking acetylation signaling to genome maintenance and cancer","volume":"12","author":"Gong","year":"2016","journal-title":"PLoS Genet."},{"key":"2022082600051784000_B34","doi-asserted-by":"crossref","first-page":"146","DOI":"10.1016\/j.tips.2011.12.002","article-title":"Place your BETs: the therapeutic potential of bromodomains","volume":"33","author":"Prinjha","year":"2012","journal-title":"Trends Pharmacol. Sci."},{"key":"2022082600051784000_B35","doi-asserted-by":"crossref","first-page":"210","DOI":"10.1016\/j.ccr.2014.01.028","article-title":"Disrupting the interaction of BRD4 with diacetylated twist suppresses tumorigenesis in basal-like breast cancer","volume":"25","author":"Shi","year":"2014","journal-title":"Cancer Cell"},{"key":"2022082600051784000_B36","doi-asserted-by":"crossref","first-page":"1068","DOI":"10.1016\/j.molcel.2016.12.022","article-title":"Distinct roles of brd2 and brd4 in potentiating the transcriptional program for Th17 cell differentiation","volume":"65","author":"Cheung","year":"2017","journal-title":"Mol. Cell"},{"key":"2022082600051784000_B37","doi-asserted-by":"crossref","first-page":"8758","DOI":"10.1073\/pnas.1433065100","article-title":"The double bromodomain protein brd4 binds to acetylated chromatin during interphase and mitosis","volume":"100","author":"Dey","year":"2003","journal-title":"Proc. Natl. Acad. Sci. U.S.A."},{"key":"2022082600051784000_B38","doi-asserted-by":"crossref","first-page":"1114","DOI":"10.1016\/j.molcel.2020.04.034","article-title":"Opposing functions of BRD4 isoforms in breast cancer","volume":"78","author":"Wu","year":"2020","journal-title":"Mol Cell"},{"key":"2022082600051784000_B39","doi-asserted-by":"crossref","first-page":"1035","DOI":"10.3389\/fmicb.2017.01035","article-title":"A novel bromodomain inhibitor reverses HIV-1 latency through specific binding with BRD4 to promote tat and P-TEFb association","volume":"8","author":"Huang","year":"2017","journal-title":"Front. Microbiol."},{"key":"2022082600051784000_B40","doi-asserted-by":"crossref","first-page":"e00452\u201320","DOI":"10.1128\/MCB.00452-20","article-title":"Proteasomal regulation of mammalian SPT16 in controlling transcription","volume":"41","author":"Kaja","year":"2021","journal-title":"Mol. Cell Biol."},{"key":"2022082600051784000_B41","doi-asserted-by":"crossref","first-page":"329","DOI":"10.1136\/gutjnl-2019-318668","article-title":"Histone chaperone FACT complex mediates oxidative stress response to promote liver cancer progression","volume":"69","author":"Shen","year":"2020","journal-title":"Gut"},{"key":"2022082600051784000_B42","doi-asserted-by":"crossref","first-page":"807","DOI":"10.1016\/j.celrep.2012.09.008","article-title":"Reactivation of latent HIV-1 by inhibition of BRD4","volume":"2","author":"Zhu","year":"2012","journal-title":"Cell Rep"},{"key":"2022082600051784000_B43","doi-asserted-by":"crossref","first-page":"47","DOI":"10.1186\/1742-4690-6-47","article-title":"In vitro nuclear interactome of the HIV-1 tat protein","volume":"6","author":"Gautier","year":"2009","journal-title":"Retrovirology"},{"key":"2022082600051784000_B44","doi-asserted-by":"crossref","first-page":"1313","DOI":"10.1101\/gad.275073.115","article-title":"The CENP-T\/-W complex is a binding partner of the histone chaperone FACT","volume":"30","author":"Prendergast","year":"2016","journal-title":"Genes Dev"},{"key":"2022082600051784000_B45","doi-asserted-by":"crossref","first-page":"304","DOI":"10.1016\/j.molcel.2014.12.009","article-title":"Developmental control of polycomb subunit composition by GATA factors mediates a switch to non-canonical functions","volume":"57","author":"Xu","year":"2015","journal-title":"Mol. Cell"},{"key":"2022082600051784000_B46","doi-asserted-by":"crossref","first-page":"47733","DOI":"10.1074\/jbc.M105590200","article-title":"Histone deacetylase 1 phosphorylation promotes enzymatic activity and complex formation","volume":"276","author":"Pflum","year":"2001","journal-title":"J. Biol. Chem."},{"key":"2022082600051784000_B47","doi-asserted-by":"crossref","first-page":"343","DOI":"10.1038\/nature09784","article-title":"The polycomb complex PRC2 and its mark in life","volume":"469","author":"Margueron","year":"2011","journal-title":"Nature"},{"key":"2022082600051784000_B48","doi-asserted-by":"crossref","first-page":"19266","DOI":"10.1073\/pnas.1008937107","article-title":"Binding of different histone marks differentially regulates the activity and specificity of polycomb repressive complex 2 (PRC2)","volume":"107","author":"Xu","year":"2010","journal-title":"Proc. Natl. Acad. Sci. U.S.A."},{"key":"2022082600051784000_B49","doi-asserted-by":"crossref","first-page":"277","DOI":"10.1016\/j.molcel.2013.12.005","article-title":"The histone H3 lysine 9 methyltransferases G9a and GLP regulate polycomb repressive complex 2-mediated gene silencing","volume":"53","author":"Mozzetta","year":"2014","journal-title":"Mol. Cell"},{"key":"2022082600051784000_B50","doi-asserted-by":"crossref","first-page":"aad7993","DOI":"10.1126\/science.aad7993","article-title":"Topoisomerase 1 inhibition suppresses inflammatory genes and protects from death by inflammation","volume":"352","author":"Rialdi","year":"2016","journal-title":"Science"},{"key":"2022082600051784000_B51","doi-asserted-by":"crossref","first-page":"e1008429","DOI":"10.1371\/journal.ppat.1008429","article-title":"BRD4 inhibition exerts anti-viral activity through DNA damage-dependent innate immune responses","volume":"16","author":"Wang","year":"2020","journal-title":"PLoS Pathog"},{"key":"2022082600051784000_B52","doi-asserted-by":"crossref","first-page":"46","DOI":"10.1186\/s13072-019-0288-3","article-title":"Interplay between FACT subunit SPT16 and TRIM33 can remodel chromatin at macrophage distal regulatory elements","volume":"12","author":"Ferri","year":"2019","journal-title":"Epigenetics Chromatin"},{"key":"2022082600051784000_B53","doi-asserted-by":"crossref","first-page":"1474","DOI":"10.1101\/gad.343038.120","article-title":"The transcription factor EGR2 is the molecular linchpin connecting STAT6 activation to the late, stable epigenomic program of alternative macrophage polarization","volume":"34","author":"Daniel","year":"2020","journal-title":"Genes Dev"},{"key":"2022082600051784000_B54","doi-asserted-by":"crossref","first-page":"974","DOI":"10.1016\/j.cell.2015.07.011","article-title":"Inhibiting DNA methylation causes an interferon response in cancer via dsRNA including endogenous retroviruses","volume":"162","author":"Chiappinelli","year":"2015","journal-title":"Cell"},{"key":"2022082600051784000_B55","doi-asserted-by":"crossref","first-page":"11622","DOI":"10.1093\/nar\/gkx746","article-title":"Histone H1 depletion triggers an interferon response in cancer cells via activation of heterochromatic repeats","volume":"45","author":"Izquierdo-Bouldstridge","year":"2017","journal-title":"Nucleic Acids Res."},{"key":"2022082600051784000_B56","doi-asserted-by":"crossref","first-page":"286","DOI":"10.1038\/s41580-021-00430-1","article-title":"Cellular origins of dsRNA, their recognition and consequences","volume":"23","author":"Chen","year":"2022","journal-title":"Nat. Rev. Mol. Cell Biol."},{"key":"2022082600051784000_B57","doi-asserted-by":"crossref","first-page":"313","DOI":"10.1038\/nm.3082","article-title":"An inhibitor of the protein kinases TBK1 and IKK-varepsilon improves obesity-related metabolic dysfunctions in mice","volume":"19","author":"Reilly","year":"2013","journal-title":"Nat. Med."},{"key":"2022082600051784000_B58","doi-asserted-by":"crossref","first-page":"780","DOI":"10.1021\/acsmedchemlett.9b00027","article-title":"Discovery of GSK8612, a highly selective and potent TBK1 inhibitor","volume":"10","author":"Thomson","year":"2019","journal-title":"ACS Med. Chem. Lett."},{"key":"2022082600051784000_B59","doi-asserted-by":"crossref","first-page":"426","DOI":"10.1038\/s41586-019-1820-0","article-title":"FACT caught in the act of manipulating the nucleosome","volume":"577","author":"Liu","year":"2020","journal-title":"Nature"},{"key":"2022082600051784000_B60","doi-asserted-by":"crossref","first-page":"111","DOI":"10.1038\/nature12242","article-title":"Structural basis of histone H2A-H2B recognition by the essential chaperone FACT","volume":"499","author":"Hondele","year":"2013","journal-title":"Nature"},{"key":"2022082600051784000_B61","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1101\/gad.1975011","article-title":"Intergenic transcription causes repression by directing nucleosome assembly","volume":"25","author":"Hainer","year":"2011","journal-title":"Genes Dev."},{"key":"2022082600051784000_B62","doi-asserted-by":"crossref","first-page":"5","DOI":"10.1016\/S0092-8674(00)81316-1","article-title":"Histone acetylation and chromatin assembly: a single escort, multiple dances?","volume":"87","author":"Roth","year":"1996","journal-title":"Cell"},{"key":"2022082600051784000_B63","doi-asserted-by":"crossref","first-page":"6927","DOI":"10.1073\/pnas.1120422109","article-title":"BRD4 is an atypical kinase that phosphorylates serine2 of the RNA polymerase II carboxy-terminal domain","volume":"109","author":"Devaiah","year":"2012","journal-title":"Proc. Natl. Acad. Sci. U.S.A."},{"key":"2022082600051784000_B64","doi-asserted-by":"crossref","first-page":"306","DOI":"10.1126\/science.1118947","article-title":"Akt-mediated phosphorylation of EZH2 suppresses methylation of lysine 27 in histone h3","volume":"310","author":"Cha","year":"2005","journal-title":"Science"},{"key":"2022082600051784000_B65","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1038\/ncb2139","article-title":"CDK1-dependent phosphorylation of EZH2 suppresses methylation of H3K27 and promotes osteogenic differentiation of human mesenchymal stem cells","volume":"13","author":"Wei","year":"2011","journal-title":"Nat. Cell Biol."},{"key":"2022082600051784000_B66","doi-asserted-by":"crossref","first-page":"1108","DOI":"10.1038\/ncb2116","article-title":"Cyclin-dependent kinases regulate epigenetic gene silencing through phosphorylation of EZH2","volume":"12","author":"Chen","year":"2010","journal-title":"Nat. Cell Biol."},{"key":"2022082600051784000_B67","doi-asserted-by":"crossref","first-page":"e00007\u201317","DOI":"10.1128\/JVI.00007-17","article-title":"BRD4 couples NF-kappaB\/RelA with airway inflammation and the IRF-RIG-I amplification loop in respiratory syncytial virus infection","volume":"91","author":"Tian","year":"2017","journal-title":"J. Virol."},{"key":"2022082600051784000_B68","doi-asserted-by":"crossref","first-page":"1375","DOI":"10.1128\/MCB.01365-08","article-title":"Brd4 coactivates transcriptional activation of NF-kappaB via specific binding to acetylated relA","volume":"29","author":"Huang","year":"2009","journal-title":"Mol. Cell Biol."},{"key":"2022082600051784000_B69","doi-asserted-by":"crossref","first-page":"129","DOI":"10.1016\/j.cell.2009.05.047","article-title":"Control of inducible gene expression by signal-dependent transcriptional elongation","volume":"138","author":"Hargreaves","year":"2009","journal-title":"Cell"},{"key":"2022082600051784000_B70","doi-asserted-by":"crossref","first-page":"2383","DOI":"10.1101\/gad.1448206","article-title":"Brd4 links chromatin targeting to HPV transcriptional silencing","volume":"20","author":"Wu","year":"2006","journal-title":"Genes Dev"},{"key":"2022082600051784000_B71","doi-asserted-by":"crossref","first-page":"e01644\u201316","DOI":"10.1128\/mBio.01644-16","article-title":"Brd4 activates early viral transcription upon human papillomavirus 18 infection of primary keratinocytes","volume":"7","author":"McKinney","year":"2016","journal-title":"Mbio"},{"key":"2022082600051784000_B72","doi-asserted-by":"crossref","first-page":"535","DOI":"10.1016\/j.molcel.2005.06.029","article-title":"Recruitment of P-TEFb for stimulation of transcriptional elongation by the bromodomain protein brd4","volume":"19","author":"Yang","year":"2005","journal-title":"Mol Cell"},{"key":"2022082600051784000_B73","doi-asserted-by":"crossref","first-page":"13690","DOI":"10.1073\/pnas.0705053104","article-title":"Conserved P-TEFb-interacting domain of BRD4 inhibits HIV transcription","volume":"104","author":"Bisgrove","year":"2007","journal-title":"Proc. Natl. Acad. Sci. U.S.A."},{"key":"2022082600051784000_B74","doi-asserted-by":"crossref","first-page":"277","DOI":"10.1093\/nar\/gks976","article-title":"The BET bromodomain inhibitor JQ1 activates HIV latency through antagonizing brd4 inhibition of Tat-transactivation","volume":"41","author":"Li","year":"2013","journal-title":"Nucleic Acids Res."},{"key":"2022082600051784000_B75","doi-asserted-by":"crossref","first-page":"3361","DOI":"10.1172\/JCI120633","article-title":"Structure-guided drug design identifies a BRD4-selective small molecule that suppresses HIV","volume":"129","author":"Niu","year":"2019","journal-title":"J. Clin. Invest."},{"key":"2022082600051784000_B76","doi-asserted-by":"crossref","first-page":"1001","DOI":"10.1016\/j.molcel.2017.07.025","article-title":"The short isoform of BRD4 promotes HIV-1 latency by engaging repressive SWI\/SNF chromatin-remodeling complexes","volume":"67","author":"Conrad","year":"2017","journal-title":"Mol. Cell"},{"key":"2022082600051784000_B77","doi-asserted-by":"crossref","first-page":"e02032\u201320","DOI":"10.1128\/JVI.02032-20","article-title":"BRD4S interacts with viral E2 protein to limit human papillomavirus late transcription","volume":"95","author":"Yigitliler","year":"2021","journal-title":"J Virol."},{"key":"2022082600051784000_B78","doi-asserted-by":"crossref","first-page":"4083","DOI":"10.1038\/s41467-020-17503-y","article-title":"BRD4 prevents the accumulation of R-loops and protects against transcription-replication collision events and DNA damage","volume":"11","author":"Lam","year":"2020","journal-title":"Nat. Commun."},{"key":"2022082600051784000_B79","doi-asserted-by":"crossref","first-page":"517","DOI":"10.1016\/j.molcel.2017.04.027","article-title":"Bromodomain protein BRD4 is a transcriptional repressor of autophagy and lysosomal function","volume":"66","author":"Sakamaki","year":"2017","journal-title":"Mol. Cell"},{"key":"2022082600051784000_B80","doi-asserted-by":"crossref","first-page":"151","DOI":"10.1016\/S0968-0004(97)01042-6","article-title":"The bromodomain revisited","volume":"22","author":"Jeanmougin","year":"1997","journal-title":"Trends Biochem. Sci."},{"key":"2022082600051784000_B81","doi-asserted-by":"crossref","first-page":"7642","DOI":"10.1093\/nar\/gku485","article-title":"Silencing of IFN-stimulated gene transcription is regulated by histone H1 and its chaperone TAF-I","volume":"42","author":"Kadota","year":"2014","journal-title":"Nucleic Acids Res."},{"key":"2022082600051784000_B82","doi-asserted-by":"crossref","first-page":"e1007667","DOI":"10.1371\/journal.ppat.1007667","article-title":"The histone chaperone HIRA promotes the induction of host innate immune defences in response to HSV-1 infection","volume":"15","author":"McFarlane","year":"2019","journal-title":"PLoS Pathog"},{"key":"2022082600051784000_B83","doi-asserted-by":"crossref","first-page":"95ra74","DOI":"10.1126\/scitranslmed.3002530","article-title":"Curaxins: anticancer compounds that simultaneously suppress NF-kappaB and activate p53 by targeting FACT","volume":"3","author":"Gasparian","year":"2011","journal-title":"Sci. Transl. Med."},{"key":"2022082600051784000_B84","doi-asserted-by":"crossref","first-page":"6620","DOI":"10.1158\/0008-5472.CAN-15-2764","article-title":"Preclinical validation of a single-treatment infusion modality that can eradicate extremity melanomas","volume":"76","author":"Kim","year":"2016","journal-title":"Cancer Res."},{"key":"2022082600051784000_B85","first-page":"186","article-title":"Anticancer drug candidate CBL0137, which inhibits histone chaperone FACT, is efficacious in preclinical orthotopic models of temozolomide-responsive and -resistant glioblastoma","volume":"19","author":"Barone","year":"2017","journal-title":"Neuro Oncol."},{"key":"2022082600051784000_B86","doi-asserted-by":"crossref","first-page":"312ra176","DOI":"10.1126\/scitranslmed.aab1803","article-title":"Therapeutic targeting of the MYC signal by inhibition of histone chaperone FACT in neuroblastoma","volume":"7","author":"Carter","year":"2015","journal-title":"Sci. Transl. Med."},{"key":"2022082600051784000_B87","doi-asserted-by":"crossref","first-page":"9019","DOI":"10.1021\/jm501120z","article-title":"Biased multicomponent reactions to develop novel bromodomain inhibitors","volume":"57","author":"McKeown","year":"2014","journal-title":"J. Med. Chem."}],"container-title":["Nucleic Acids Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/nar\/article-pdf\/50\/15\/8700\/45508216\/gkac645.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/nar\/article-pdf\/50\/15\/8700\/45508216\/gkac645.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,8,25]],"date-time":"2022-08-25T20:06:45Z","timestamp":1661458005000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/nar\/article\/50\/15\/8700\/6651879"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,7,29]]},"references-count":87,"journal-issue":{"issue":"15","published-online":{"date-parts":[[2022,7,29]]},"published-print":{"date-parts":[[2022,8,26]]}},"URL":"https:\/\/doi.org\/10.1093\/nar\/gkac645","relation":{"has-preprint":[{"id-type":"doi","id":"10.1101\/2021.04.21.440833","asserted-by":"object"}]},"ISSN":["0305-1048","1362-4962"],"issn-type":[{"value":"0305-1048","type":"print"},{"value":"1362-4962","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2022,8,26]]},"published":{"date-parts":[[2022,7,29]]}}}