{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,4]],"date-time":"2025-11-04T11:03:51Z","timestamp":1762254231107,"version":"3.37.3"},"reference-count":36,"publisher":"Oxford University Press (OUP)","issue":"19","license":[{"start":{"date-parts":[[2022,8,27]],"date-time":"2022-08-27T00:00:00Z","timestamp":1661558400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004329","name":"Slovenian Research Agency","doi-asserted-by":"publisher","award":["J1-8150","J4-1778","P1-0060","P1-0207","P4-0077","P1-0198"],"award-info":[{"award-number":["J1-8150","J4-1778","P1-0060","P1-0207","P4-0077","P1-0198"]}],"id":[{"id":"10.13039\/501100004329","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2022,10,28]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Encapsulation of a selected DNA molecule in a cell has important implications for bionanotechnology. Non-viral proteins that can be used as nucleic acid containers include proteinaceous subcellular bacterial microcompartments (MCPs) that self-assemble into a selectively permeable protein shell containing an enzymatic core. Here, we adapted a propanediol utilization (Pdu) MCP into a synthetic protein cage to package a specified DNA segment in vivo, thereby enabling subsequent affinity purification. To this end, we engineered the LacI transcription repressor to be routed, together with target DNA, into the lumen of a Strep-tagged Pdu shell. Sequencing of extracted DNA from the affinity-isolated MCPs shows that our strategy results in packaging of a DNA segment carrying multiple LacI binding sites, but not the flanking regions. Furthermore, we used LacI to drive the encapsulation of a DNA segment containing operators for LacI and for a second transcription factor.<\/jats:p>","DOI":"10.1093\/nar\/gkac714","type":"journal-article","created":{"date-parts":[[2022,8,27]],"date-time":"2022-08-27T10:08:43Z","timestamp":1661594923000},"page":"e113-e113","source":"Crossref","is-referenced-by-count":3,"title":["A method for targeting a specified segment of DNA to a bacterial microorganelle"],"prefix":"10.1093","volume":"50","author":[{"given":"Jan","family":"Otoni\u010dar","sequence":"first","affiliation":[{"name":"Department of Biology, Biotechnical Faculty, University of Ljubljana ,\u00a01000\u00a0 Ljubljana ,\u00a0 Slovenia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maja","family":"Hostnik","sequence":"additional","affiliation":[{"name":"Department of Biology, Biotechnical Faculty, University of Ljubljana ,\u00a01000\u00a0 Ljubljana ,\u00a0 Slovenia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maja","family":"Grundner","sequence":"additional","affiliation":[{"name":"Department of Biology, Biotechnical Faculty, University of Ljubljana ,\u00a01000\u00a0 Ljubljana ,\u00a0 Slovenia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rok","family":"Kostanj\u0161ek","sequence":"additional","affiliation":[{"name":"Department of Biology, Biotechnical Faculty, University of Ljubljana ,\u00a01000\u00a0 Ljubljana ,\u00a0 Slovenia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tajda","family":"Gredar","sequence":"additional","affiliation":[{"name":"Department of Biology, Biotechnical Faculty, University of Ljubljana ,\u00a01000\u00a0 Ljubljana ,\u00a0 Slovenia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maja","family":"Garvas","sequence":"additional","affiliation":[{"name":"Jo\u017eef Stefan Institute, Condensed Matter Physics Department ,\u00a01000\u00a0 Ljubljana ,\u00a0 Slovenia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zoran","family":"Arsov","sequence":"additional","affiliation":[{"name":"Jo\u017eef Stefan Institute, Condensed Matter Physics Department ,\u00a01000\u00a0 Ljubljana ,\u00a0 Slovenia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zdravko","family":"Podlesek","sequence":"additional","affiliation":[{"name":"Department of Biology, Biotechnical Faculty, University of Ljubljana ,\u00a01000\u00a0 Ljubljana ,\u00a0 Slovenia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cene","family":"Gostin\u010dar","sequence":"additional","affiliation":[{"name":"Department of Biology, Biotechnical Faculty, University of Ljubljana ,\u00a01000\u00a0 Ljubljana ,\u00a0 Slovenia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jernej","family":"Jak\u0161e","sequence":"additional","affiliation":[{"name":"Department of Agronomy, Biotechnical Faculty, University of Ljubljana ,\u00a01000\u00a0 Ljubljana ,\u00a0 Slovenia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2148-1758","authenticated-orcid":false,"given":"Stephen J W","family":"Busby","sequence":"additional","affiliation":[{"name":"School of Biosciences, University of Birmingham , Birmingham \u00a0B15 2TT,\u00a0 UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2411-3221","authenticated-orcid":false,"given":"Matej","family":"Butala","sequence":"additional","affiliation":[{"name":"Department of Biology, Biotechnical Faculty, University of Ljubljana ,\u00a01000\u00a0 Ljubljana ,\u00a0 Slovenia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2022,8,27]]},"reference":[{"key":"2022110710200170200_B1","doi-asserted-by":"crossref","first-page":"677","DOI":"10.1038\/s41579-020-0413-0","article-title":"Formation and function of bacterial organelles","volume":"18","author":"Greening","year":"2020","journal-title":"Nat. Rev. Microbiol."},{"key":"2022110710200170200_B2","doi-asserted-by":"crossref","first-page":"277","DOI":"10.1038\/nrmicro.2018.10","article-title":"Bacterial microcompartments","volume":"16","author":"Kerfeld","year":"2018","journal-title":"Nat. Rev. Microbiol."},{"key":"2022110710200170200_B3","doi-asserted-by":"crossref","first-page":"325","DOI":"10.1016\/j.tibtech.2018.08.006","article-title":"Biotechnological advances in bacterial microcompartment technology","volume":"37","author":"Lee","year":"2019","journal-title":"Trends Biotechnol"},{"key":"2022110710200170200_B4","doi-asserted-by":"crossref","first-page":"e1006351","DOI":"10.1371\/journal.pcbi.1006351","article-title":"The role of the encapsulated cargo in microcompartment assembly","volume":"14","author":"Mohajerani","year":"2018","journal-title":"PLoS Comput. Biol."},{"key":"2022110710200170200_B5","doi-asserted-by":"crossref","first-page":"e1010","DOI":"10.1002\/mbo3.1010","article-title":"Effect of metabolosome encapsulation peptides on enzyme activity, coaggregation, incorporation, and bacterial microcompartment formation","volume":"9","author":"Juodeikis","year":"2020","journal-title":"Microbiol. Open"},{"key":"2022110710200170200_B6","doi-asserted-by":"crossref","first-page":"5967","DOI":"10.1128\/JB.181.19.5967-5975.1999","article-title":"The propanediol utilization (pdu) operon of Salmonellaenterica serovar Typhimurium LT2 includes genes necessary for formation of polyhedral organelles involved in coenzyme B12-dependent 1,2-propanediol degradation","volume":"181","author":"Bobik","year":"1999","journal-title":"J. Bacteriol."},{"key":"2022110710200170200_B7","doi-asserted-by":"crossref","first-page":"14366","DOI":"10.1074\/jbc.M709214200","article-title":"Biochemical and structural insights into bacterial organelle form and biogenesis","volume":"283","author":"Parsons","year":"2008","journal-title":"J. Biol. Chem."},{"key":"2022110710200170200_B8","doi-asserted-by":"crossref","first-page":"37838","DOI":"10.1074\/jbc.M110.160580","article-title":"Structural insight into the mechanisms of transport across the Salmonellaenterica Pdu microcompartment shell","volume":"285","author":"Crowley","year":"2010","journal-title":"J. Biol. Chem."},{"key":"2022110710200170200_B9","first-page":"1355","article-title":"Engineering the PduT shell protein to modify the permeability of the 1,2-propanediol microcompartment of Salmonella","volume":"165","author":"Chowdhury","year":"2019","journal-title":"Microbiol. 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