{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T04:09:19Z","timestamp":1777435759684,"version":"3.51.4"},"reference-count":53,"publisher":"Oxford University Press (OUP)","issue":"17","license":[{"start":{"date-parts":[[2022,9,12]],"date-time":"2022-09-12T00:00:00Z","timestamp":1662940800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001691","name":"JSPS","doi-asserted-by":"publisher","award":["26000007"],"award-info":[{"award-number":["26000007"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Scientific Research","award":["19H00924"],"award-info":[{"award-number":["19H00924"]}]},{"name":"Scientific Research","award":["22H00351"],"award-info":[{"award-number":["22H00351"]}]},{"name":"Scientific research for Young Scientist","award":["17K14516"],"award-info":[{"award-number":["17K14516"]}]},{"name":"Scientific Research","award":["19K05693"],"award-info":[{"award-number":["19K05693"]}]},{"name":"Scientific Research","award":["22K05315"],"award-info":[{"award-number":["22K05315"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2022,9,23]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Trinucleotide repeat (TNR) diseases are caused by the aberrant expansion of CXG (X\u00a0=\u00a0C, A, G\u00a0and T) sequences in genomes. We have reported two small molecules binding to TNR, NCD, and NA, which strongly bind to CGG repeat (responsible sequence of fragile X syndrome) and CAG repeat (Huntington's disease). The NMR structure of NA binding to the CAG\/CAG triad has been clarified, but the structure of NCD bound to the CGG\/CGG triad remained to be addressed. We here report the structural determination of the NCD-CGG\/CGG complex by NMR spectroscopy and the comparison with the NA-CAG\/CAG complex. While the NCD-CGG\/CGG structure shares the binding characteristics with that of the NA-CAG\/CAG complex, a significant difference was found in the overall structure caused by the structural fluctuation at the ligand-bound site. The NCD-CGG\/CGG complex was suggested in the equilibrium between stacked and kinked structures, although NA-CAG\/CAG complex has only the stacked structures. The dynamic fluctuation of the NCD-CGG\/CGG structure at the NCD-binding site suggested room for optimization in the linker structure of NCD to gain improved affinity to the CGG\/CGG triad.<\/jats:p>","DOI":"10.1093\/nar\/gkac740","type":"journal-article","created":{"date-parts":[[2022,9,12]],"date-time":"2022-09-12T18:14:42Z","timestamp":1663006482000},"page":"9621-9631","source":"Crossref","is-referenced-by-count":13,"title":["NMR determination of the 2:1 binding complex of naphthyridine carbamate dimer (NCD) and CGG\/CGG triad in double-stranded DNA"],"prefix":"10.1093","volume":"50","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3275-415X","authenticated-orcid":false,"given":"Takeshi","family":"Yamada","sequence":"first","affiliation":[{"name":"Department of Regulatory Bioorganic Chemistry, SANKEN, Osaka University , 8-1 Mihogaoka, Ibaraki 567-0047, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kyoko","family":"Furuita","sequence":"additional","affiliation":[{"name":"Institute for Protein Research, Osaka University , 3-2 Yamadaoka, Suita, Osaka 565-0871, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6561-2356","authenticated-orcid":false,"given":"Shuhei","family":"Sakurabayashi","sequence":"additional","affiliation":[{"name":"Department of Regulatory Bioorganic Chemistry, SANKEN, Osaka University , 8-1 Mihogaoka, Ibaraki 567-0047, Japan"},{"name":"Institute for Protein Research, Osaka University , 3-2 Yamadaoka, Suita, Osaka 565-0871, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Makoto","family":"Nomura","sequence":"additional","affiliation":[{"name":"Graduate School of Biological Sciences, Nara Institute of Science and Technology , 8916-5 Takayama, Ikoma 630-0192, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chojiro","family":"Kojima","sequence":"additional","affiliation":[{"name":"Institute for Protein Research, Osaka University , 3-2 Yamadaoka, Suita, Osaka 565-0871, Japan"},{"name":"Graduate School of Engineering Science, Yokohama National University , 79-5 Tokiwadai, Hodogaya-ku, Yokohama 240-8501, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1705-5265","authenticated-orcid":false,"given":"Kazuhiko","family":"Nakatani","sequence":"additional","affiliation":[{"name":"Department of Regulatory Bioorganic Chemistry, SANKEN, Osaka University , 8-1 Mihogaoka, Ibaraki 567-0047, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2022,9,12]]},"reference":[{"key":"2022092413204561200_B1","doi-asserted-by":"crossref","first-page":"533","DOI":"10.1016\/0092-8674(95)90074-8","article-title":"Trinucleotide repeats that expand in human disease form hairpin structures in vitro","volume":"81","author":"Gacy","year":"1995","journal-title":"Cell"},{"key":"2022092413204561200_B2","doi-asserted-by":"crossref","first-page":"28970","DOI":"10.1074\/jbc.270.48.28970","article-title":"The fragile X syndrome single strand d(CGG)n nucleotide repeats readily fold back to form unimolecular hairpin structures","volume":"270","author":"Nadel","year":"1995","journal-title":"J. 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Chem."},{"key":"2022092413204561200_B5","doi-asserted-by":"crossref","first-page":"905","DOI":"10.1016\/0092-8674(91)90397-H","article-title":"Identification of a gene (FMR-1) containing a CGG repeat coincident with a breakpoint cluster region exhibiting length variation in fragile X syndrome","volume":"65","author":"Verkerk","year":"1991","journal-title":"Cell"},{"key":"2022092413204561200_B6","doi-asserted-by":"crossref","first-page":"278","DOI":"10.1016\/S0959-437X(02)00299-X","article-title":"The fragile X premutation: into the phenotypic fold","volume":"12","author":"Hagerman","year":"2002","journal-title":"Curr. Opin. Genet. Dev."},{"key":"2022092413204561200_B7","doi-asserted-by":"crossref","first-page":"703","DOI":"10.1146\/annurev.ge.29.120195.003415","article-title":"Trinucleotide repeat expansion and human disease","volume":"29","author":"Ashley","year":"1995","journal-title":"Annu. Rev. 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