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However, insufficient large-scale integrated databases hinder researchers from accessing and exploring single-cell proteomics, impeding the advancement of this field. To fill this deficiency, we present a comprehensive database, namely Single-cell Proteomic DataBase (SPDB, https:\/\/scproteomicsdb.com\/), for general single-cell proteomic data, including antibody-based or mass spectrometry-based single-cell proteomics. Equipped with standardized data process and a user-friendly web interface, SPDB provides unified data formats for convenient interaction with downstream analysis, and offers not only dataset-level but also protein-level data search and exploration capabilities. To enable detailed exhibition of single-cell proteomic data, SPDB also provides a module for visualizing data from the perspectives of cell metadata or protein features. The current version of SPDB encompasses 133 antibody-based single-cell proteomic datasets involving more than 300 million cells and over 800 marker\/surface proteins, and 10 mass spectrometry-based single-cell proteomic datasets involving more than 4000 cells and over 7000 proteins. Overall, SPDB is envisioned to be explored as a useful resource that will facilitate the wider research communities by providing detailed insights into proteomics from the single-cell perspective.<\/jats:p>","DOI":"10.1093\/nar\/gkad1018","type":"journal-article","created":{"date-parts":[[2023,11,13]],"date-time":"2023-11-13T03:58:16Z","timestamp":1699847896000},"page":"D562-D571","source":"Crossref","is-referenced-by-count":20,"title":["SPDB: a comprehensive resource and knowledgebase for proteomic data at the single-cell resolution"],"prefix":"10.1093","volume":"52","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1491-5207","authenticated-orcid":false,"given":"Fang","family":"Wang","sequence":"first","affiliation":[{"name":"School of Computer Science and Technology, Harbin Institute of Technology , Harbin\u00a0 150001 , China"},{"name":"AI Lab, Tencent , Shenzhen\u00a0 518000 , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chunpu","family":"Liu","sequence":"additional","affiliation":[{"name":"School of Computer Science and Technology, Harbin Institute of Technology , Harbin\u00a0 150001 , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiawei","family":"Li","sequence":"additional","affiliation":[{"name":"College of Intelligence and Computing, Tianjin University , Tianjin\u00a0 300350 , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fan","family":"Yang","sequence":"additional","affiliation":[{"name":"AI Lab, Tencent , Shenzhen\u00a0 518000 , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8031-9086","authenticated-orcid":false,"given":"Jiangning","family":"Song","sequence":"additional","affiliation":[{"name":"Biomedicine Discovery Institute and Department of Biochemistry and Molecular Biology, Monash University , Melbourne , VIC 3800 , Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tianyi","family":"Zang","sequence":"additional","affiliation":[{"name":"School of Computer Science and Technology, Harbin Institute of Technology , Harbin\u00a0 150001 , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianhua","family":"Yao","sequence":"additional","affiliation":[{"name":"AI Lab, Tencent , Shenzhen\u00a0 518000 , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guohua","family":"Wang","sequence":"additional","affiliation":[{"name":"School of Computer Science and Technology, Harbin Institute of Technology , Harbin\u00a0 150001 , China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2023,11,11]]},"reference":[{"key":"2024010511374544500_B1","doi-asserted-by":"crossref","first-page":"365","DOI":"10.1146\/annurev-bioeng-060418-052538","article-title":"Single-cell omics analyses enabled by microchip technologies","volume":"21","author":"Deng","year":"2019","journal-title":"Annu. 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