{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T13:49:48Z","timestamp":1791899388553,"version":"4.3.4"},"reference-count":95,"publisher":"Oxford University Press (OUP)","issue":"8","license":[{"start":{"date-parts":[[2023,4,7]],"date-time":"2023-04-07T00:00:00Z","timestamp":1680825600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000060","name":"National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["R01AI128940"],"award-info":[{"award-number":["R01AI128940"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000060","name":"National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["U19AI089672"],"award-info":[{"award-number":["U19AI089672"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000060","name":"National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["R21AI149202"],"award-info":[{"award-number":["R21AI149202"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100011280","name":"Morsani College of Medicine","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100011280","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100008900","name":"University of South Florida","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100008900","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Cohen Foundation scholarship"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2023,5,8]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>DNA modifications are critical in fine-tuning the biological processes in model organisms. However, the presence of cytosine methylation (5mC) and the function of the putative DNA methyltransferase, PfDNMT2, in the human malaria pathogen, Plasmodium falciparum, remain controversial. Here, we revisited the 5mC in the parasite genome and the function of PfDNMT2. Low levels of genomic 5mC (0.1\u20130.2%) during asexual development were identified using a sensitive mass spectrometry procedure. Native PfDNMT2 displayed substantial DNA methylation activities, and disruption or overexpression of PfDNMT2 resulted in reduced or elevated genomic 5mC levels, respectively. PfDNMT2 disruption led to an increased proliferation phenotype, with the parasites having an extended schizont stage and producing a higher number of progenies. Consistent with PfDNMT2\u2019s interaction with an AP2 domain-containing transcription factor, transcriptomic analyses revealed that PfDNMT2 disruption led to a drastic alteration in the expression of many genes, some of which provided the molecular basis of enhanced proliferation after PfDNMT2 disruption. Furthermore, levels of tRNAAsp and its methylation rate at position C38, and the translation of a reporter containing an aspartate repeat were significantly reduced after PfDNMT2 disruption, while the levels of tRNAAsp and its C38 methylation were restored after complementation of PfDNMT2. Our study sheds new light on the dual function of PfDNMT2 during P. falciparum asexual development.<\/jats:p>","DOI":"10.1093\/nar\/gkad248","type":"journal-article","created":{"date-parts":[[2023,4,7]],"date-time":"2023-04-07T09:28:01Z","timestamp":1680859681000},"page":"3918-3933","source":"Crossref","is-referenced-by-count":32,"title":["Characterization of the dual role of <i>Plasmodium falciparum<\/i> DNA methyltransferase in regulating transcription and translation"],"prefix":"10.1093","volume":"51","author":[{"given":"Amuza B","family":"Lucky","sequence":"first","affiliation":[{"name":"Department of Internal Medicine, Morsani College of Medicine, University of South Florida , 3720 Spectrum Blvd, Tampa , FL , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chengqi","family":"Wang","sequence":"additional","affiliation":[{"name":"Center for Global Health and Infectious Diseases Research, College of Public Health, University of South Florida , 3720 Spectrum Blvd, Tampa , FL , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaolian","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, Morsani College of Medicine, University of South Florida , 3720 Spectrum Blvd, Tampa , FL , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anongruk","family":"Chim-Ong","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, Morsani College of Medicine, University of South Florida , 3720 Spectrum Blvd, Tampa , FL , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Swamy\u00a0R","family":"Adapa","sequence":"additional","affiliation":[{"name":"Center for Global Health and Infectious Diseases Research, College of Public Health, University of South Florida , 3720 Spectrum Blvd, Tampa , FL , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eoin P","family":"Quinlivan","sequence":"additional","affiliation":[{"name":"Department of Pathology, Immunology and Laboratory Medicine, University of Florida , Gainesville , FL , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rays","family":"Jiang","sequence":"additional","affiliation":[{"name":"Center for Global Health and Infectious Diseases Research, College of Public Health, University of South Florida , 3720 Spectrum Blvd, Tampa , FL , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8338-1974","authenticated-orcid":false,"given":"Liwang","family":"Cui","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, Morsani College of Medicine, University of South Florida , 3720 Spectrum Blvd, Tampa , FL , USA"},{"name":"Center for Global Health and Infectious Diseases Research, College of Public Health, University of South Florida , 3720 Spectrum Blvd, Tampa , FL , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9374-6749","authenticated-orcid":false,"given":"Jun","family":"Miao","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, Morsani College of Medicine, University of South Florida , 3720 Spectrum Blvd, Tampa , FL , USA"},{"name":"Center for Global Health and Infectious Diseases Research, College of Public Health, University of South Florida , 3720 Spectrum Blvd, Tampa , FL , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2023,4,7]]},"reference":[{"key":"2023050800214457800_B1","doi-asserted-by":"crossref","first-page":"24","DOI":"10.1186\/s13072-015-0016-6","article-title":"Epigenetic regulatory functions of DNA modifications: 5-methylcytosine and beyond","volume":"8","author":"Breiling","year":"2015","journal-title":"Epigenetics Chromatin"},{"key":"2023050800214457800_B2","doi-asserted-by":"crossref","first-page":"484","DOI":"10.1038\/nrg3230","article-title":"Functions of DNA methylation: islands, start sites, gene bodies and beyond","volume":"13","author":"Jones","year":"2012","journal-title":"Nat. Rev. Genet."},{"key":"2023050800214457800_B3","doi-asserted-by":"crossref","first-page":"465","DOI":"10.1038\/nrg2341","article-title":"DNA methylation landscapes: provocative insights from epigenomics","volume":"9","author":"Suzuki","year":"2008","journal-title":"Nat. Rev. Genet."},{"key":"2023050800214457800_B4","doi-asserted-by":"crossref","first-page":"a019133","DOI":"10.1101\/cshperspect.a019133","article-title":"DNA methylation in mammals","volume":"6","author":"Li","year":"2014","journal-title":"Cold Spring Harb. Perspect. Biol."},{"key":"2023050800214457800_B5","doi-asserted-by":"crossref","first-page":"8689","DOI":"10.1073\/pnas.1002720107","article-title":"Conservation and divergence of methylation patterning in plants and animals","volume":"107","author":"Feng","year":"2010","journal-title":"Proc. Natl. Acad. Sci. 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