{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T23:29:15Z","timestamp":1787009355258,"version":"build-2736575974"},"reference-count":35,"publisher":"Oxford University Press (OUP)","issue":"11","license":[{"start":{"date-parts":[[2023,5,17]],"date-time":"2023-05-17T00:00:00Z","timestamp":1684281600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100002855","name":"Ministry of Science and Technology of the People's Republic of China","doi-asserted-by":"publisher","award":["2021YFB3202504"],"award-info":[{"award-number":["2021YFB3202504"]}],"id":[{"id":"10.13039\/501100002855","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002855","name":"Ministry of Science and Technology of the People's Republic of China","doi-asserted-by":"publisher","award":["2022YFE0107400"],"award-info":[{"award-number":["2022YFE0107400"]}],"id":[{"id":"10.13039\/501100002855","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003399","name":"Science and Technology Commission of Shanghai Municipality","doi-asserted-by":"publisher","award":["21NL2600100"],"award-info":[{"award-number":["21NL2600100"]}],"id":[{"id":"10.13039\/501100003399","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003399","name":"Science and Technology Commission of Shanghai Municipality","doi-asserted-by":"publisher","award":["20DZ2220500"],"award-info":[{"award-number":["20DZ2220500"]}],"id":[{"id":"10.13039\/501100003399","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Key Technology Team Project of Chinese Academy of Sciences","award":["GJJSTD20210006"],"award-info":[{"award-number":["GJJSTD20210006"]}]},{"name":"SITRI"},{"name":"Si-gene Biotech Co., Ltd"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2023,6,23]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Despite the need in various applications, accurate quantification of nucleic acids still remains a challenge. The widely-used qPCR has reduced accuracy at ultralow template concentration and is susceptible to nonspecific amplifications. The more recently developed dPCR is costly and cannot handle high-concentration samples. We combine the strengths of qPCR and dPCR by performing PCR in silicon-based microfluidic chips and demonstrate high quantification accuracy in a large concentration range. Importantly, at low template concentration, we observe on-site PCR (osPCR), where only certain sites of the channel show amplification. The sites have almost identical ct values, showing osPCR is a quasi-single molecule phenomenon. Using osPCR, we can measure both the ct values and the absolute concentration of templates in the same reaction. Additionally, osPCR enables identification of each template molecule, allowing removal of nonspecific amplification during quantification and greatly improving quantification accuracy. We develop sectioning algorithm that improves the signal amplitude and demonstrate improved detection of COVID in patient samples.<\/jats:p>","DOI":"10.1093\/nar\/gkad388","type":"journal-article","created":{"date-parts":[[2023,5,17]],"date-time":"2023-05-17T06:54:03Z","timestamp":1684306443000},"page":"e65-e65","source":"Crossref","is-referenced-by-count":9,"title":["Accurate quantification of DNA using on-site PCR (osPCR) by characterizing DNA amplification at single-molecule resolution"],"prefix":"10.1093","volume":"51","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7388-1887","authenticated-orcid":false,"given":"Ruihua","family":"Ding","sequence":"first","affiliation":[{"name":"Shanghai Industrial \u03bcTechnology Research Institute (SITRI) , Shanghai 201800 , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liying","family":"Liu","sequence":"additional","affiliation":[{"name":"Shanghai Si-Gene Biotech Co., Ltd , Shanghai 201800 , 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