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SARS-CoV-2, the etiological agent of severe acute respiratory syndrome, also prevents mRNA nuclear export. In this study, Nsp14, a bifunctional viral replicase subunit, was identified as a novel inhibitor of mRNA nuclear export. Nsp14 induces poly(A)+ RNA nuclear accumulation and the dissolution\/coalescence of nuclear speckles. Genome-wide gene expression analysis revealed the global dysregulation of splicing and 3\u2032-end processing defects of replication-dependent histone mRNAs by Nsp14. These abnormalities were also observed in SARS-CoV-2-infected cells. A mutation introduced at the guanine-N7-methyltransferase active site of Nsp14 diminished these inhibitory activities. Targeted capillary electrophoresis-mass spectrometry analysis (CE-MS) unveiled the production of N7-methyl-GTP in Nsp14-expressing cells. Association of the nuclear cap-binding complex (NCBC) with the mRNA cap and subsequent recruitment of U1 snRNP and the stem-loop binding protein (SLBP) were impaired by Nsp14. These data suggest that the defects in mRNA processing and export arise from the compromise of NCBC function by N7-methyl-GTP, thus exemplifying a novel viral strategy to block host gene expression.<\/jats:p>","DOI":"10.1093\/nar\/gkad483","type":"journal-article","created":{"date-parts":[[2023,6,1]],"date-time":"2023-06-01T07:27:56Z","timestamp":1685604476000},"page":"7602-7618","source":"Crossref","is-referenced-by-count":25,"title":["Nsp14 of SARS-CoV-2 inhibits mRNA processing and nuclear export by targeting the nuclear cap-binding complex"],"prefix":"10.1093","volume":"51","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5188-0368","authenticated-orcid":false,"given":"Jun","family":"Katahira","sequence":"first","affiliation":[{"name":"Laboratory of Cellular Molecular Biology, Graduate School of Veterinary Sciences, Osaka Metropolitan University , 1-58 Rinku-Orai-kita, Izumisano, Osaka 598-8531, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tatsuya","family":"Ohmae","sequence":"additional","affiliation":[{"name":"Laboratory of Cellular Molecular Biology, Graduate School of Veterinary Sciences, Osaka Metropolitan University , 1-58 Rinku-Orai-kita, Izumisano, Osaka 598-8531, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mayo","family":"Yasugi","sequence":"additional","affiliation":[{"name":"Laboratory of Veterinary Public Health, Graduate School of Veterinary Sciences, Osaka Metropolitan University , 1-58 Rinku-Orai-kita, Izumisano, Osaka 598-8531, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ryosuke","family":"Sasaki","sequence":"additional","affiliation":[{"name":"RIKEN Center for Sustainable Resource Science, Mass Spectrometry and Microscopy Unit , 1-7-22 Suehiro. 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