{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T05:54:39Z","timestamp":1788328479557,"version":"build-2803163510"},"reference-count":76,"publisher":"Oxford University Press (OUP)","issue":"22","license":[{"start":{"date-parts":[[2024,11,26]],"date-time":"2024-11-26T00:00:00Z","timestamp":1732579200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Italian Research Minister","award":["PRIN2022 P2022AW2H9"],"award-info":[{"award-number":["PRIN2022 P2022AW2H9"]}]},{"name":"Italian Research Minister","award":["PRIN2022 P2022K9SJ27"],"award-info":[{"award-number":["PRIN2022 P2022K9SJ27"]}]},{"DOI":"10.13039\/100000060","name":"National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["R01AI172822"],"award-info":[{"award-number":["R01AI172822"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Netherlands Organization for Scientific Research","award":["OCENW.GROOT.2019.012"],"award-info":[{"award-number":["OCENW.GROOT.2019.012"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,12,11]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>The transcriptional regulator MucR from Brucella species controls the expression of many genes, including those involved in virulence, by binding AT-rich DNA regions. MucR and its homologs belong to the Ros\/MucR family, whose members occur in \u03b1-proteobacteria. MucR is a recent addition to the family of histone-like nucleoid structuring (H-NS) proteins. Indeed, despite the lack of sequence homology, MucR bears many functional similarities with H-NS and H-NS-like proteins, structuring the bacterial genome and acting as global regulators of transcription. Here we present an integrated cryogenic electron microscopy\u00a0(cryo-EM), nuclear magnetic resonance, modeling and biochemical study shedding light on the functional architecture of MucR from Brucella abortus and its homolog Ml5 from Mesorhizobium loti. We show that MucR and Ml5 fold in a circular quaternary assembly, which allows it to bridge and condense DNA by binding AT-rich sequences. Our results show that Ros\/MucR family members are a novel type of H-NS-like proteins and, based on previous studies, provide a model connecting nucleoid structure and transcription regulation in \u03b1-proteobacteria.<\/jats:p>","DOI":"10.1093\/nar\/gkae1104","type":"journal-article","created":{"date-parts":[[2024,10,25]],"date-time":"2024-10-25T15:25:55Z","timestamp":1729869955000},"page":"13945-13963","source":"Crossref","is-referenced-by-count":8,"title":["Circular oligomeric particles formed by Ros\/MucR family members mediate DNA organization in \u03b1-proteobacteria"],"prefix":"10.1093","volume":"52","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3287-9024","authenticated-orcid":false,"given":"Antonio","family":"Chaves-Sanjuan","sequence":"first","affiliation":[{"name":"Department of Biosciences, University of Milan , Via Celoria 26 , 20133\u00a0 Milano ,","place":["Italy"]},{"name":"Fondazione Romeo e Enrica Invernizzi and NOLIMITS, University of Milan , Via Celoria 26 , 20133\u00a0 Milan ,","place":["Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gianluca","family":"D\u2019Abrosca","sequence":"additional","affiliation":[{"name":"Department of Clinical and Experimental Medicine, University of Foggia , Viale Pinto 1 , 71100\u00a0 Foggia ,","place":["Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Veronica","family":"Russo","sequence":"additional","affiliation":[{"name":"Department of Environmental, Biological, Pharmaceutical Sciences and Technologies, University of Campania \u201cLuigi Vanvitelli\u201d , Via Vivaldi 43 , 81100\u00a0 Caserta ,","place":["Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bert","family":"van\u00a0Erp","sequence":"additional","affiliation":[{"name":"Macromolecular Biochemistry, Leiden Institute of Chemistry, Leiden University , Einsteinweg 55, Leiden 2333CC,\u00a0","place":["The Netherlands"]},{"name":"Centre for Microbial Cell Biology, Leiden University ,\u00a0Einsteinweg 55, Leiden\u00a0 2333CC ,","place":["The Netherlands"]},{"name":"Centre for Interdisciplinary Genome Research, Leiden University ,\u00a0Einsteinweg 55, Leiden\u00a0 2333CC ,","place":["The Netherlands"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alessandro","family":"Del\u00a0Cont-Bernard","sequence":"additional","affiliation":[{"name":"Department of Biosciences, University of Milan , Via Celoria 26 , 20133\u00a0 Milano ,","place":["Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Riccardo","family":"Capelli","sequence":"additional","affiliation":[{"name":"Department of Biosciences, University of Milan , Via Celoria 26 , 20133\u00a0 Milano ,","place":["Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luciano","family":"Pirone","sequence":"additional","affiliation":[{"name":"Institute of Biostructures and Bioimaging, CNR, Via Pietro Castellino 111 , 80131\u00a0 Naples ,","place":["Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martina","family":"Slapakova","sequence":"additional","affiliation":[{"name":"Department of Environmental, Biological, Pharmaceutical Sciences and Technologies, University of Campania \u201cLuigi Vanvitelli\u201d , Via Vivaldi 43 , 81100\u00a0 Caserta ,","place":["Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Domenico","family":"Sgambati","sequence":"additional","affiliation":[{"name":"Department of Environmental, Biological, Pharmaceutical Sciences and Technologies, University of Campania \u201cLuigi Vanvitelli\u201d , Via Vivaldi 43 , 81100\u00a0 Caserta ,","place":["Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3539-5343","authenticated-orcid":false,"given":"Roberto","family":"Fattorusso","sequence":"additional","affiliation":[{"name":"Department of Environmental, Biological, Pharmaceutical Sciences and Technologies, University of Campania \u201cLuigi Vanvitelli\u201d , Via Vivaldi 43 , 81100\u00a0 Caserta ,","place":["Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carla","family":"Isernia","sequence":"additional","affiliation":[{"name":"Department of Environmental, Biological, Pharmaceutical Sciences and Technologies, University of Campania \u201cLuigi Vanvitelli\u201d , Via Vivaldi 43 , 81100\u00a0 Caserta ,","place":["Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luigi","family":"Russo","sequence":"additional","affiliation":[{"name":"Department of Environmental, Biological, Pharmaceutical Sciences and Technologies, University of Campania \u201cLuigi Vanvitelli\u201d , Via Vivaldi 43 , 81100\u00a0 Caserta ,","place":["Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ian\u00a0S","family":"Barton","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Brody School of Medicine, East Carolina University , 600 Moye Blvd, Greenville, NC 27834,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"suffix":"II","given":"Roy\u00a0Martin","family":"Roop","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Brody School of Medicine, East Carolina University , 600 Moye Blvd, Greenville, NC 27834,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emilia\u00a0M","family":"Pedone","sequence":"additional","affiliation":[{"name":"Institute of Biostructures and Bioimaging, CNR, Via Pietro Castellino 111 , 80131\u00a0 Naples ,","place":["Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9253-5170","authenticated-orcid":false,"given":"Martino","family":"Bolognesi","sequence":"additional","affiliation":[{"name":"Department of Biosciences, University of Milan , Via Celoria 26 , 20133\u00a0 Milano ,","place":["Italy"]},{"name":"Fondazione Romeo e Enrica Invernizzi and NOLIMITS, University of Milan , Via Celoria 26 , 20133\u00a0 Milan ,","place":["Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9863-1692","authenticated-orcid":false,"given":"Remus\u00a0T","family":"Dame","sequence":"additional","affiliation":[{"name":"Macromolecular Biochemistry, Leiden Institute of Chemistry, Leiden University , Einsteinweg 55, Leiden 2333CC,\u00a0","place":["The Netherlands"]},{"name":"Centre for Microbial Cell Biology, Leiden University ,\u00a0Einsteinweg 55, Leiden\u00a0 2333CC ,","place":["The Netherlands"]},{"name":"Centre for Interdisciplinary Genome Research, Leiden University ,\u00a0Einsteinweg 55, Leiden\u00a0 2333CC ,","place":["The Netherlands"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paolo\u00a0V","family":"Pedone","sequence":"additional","affiliation":[{"name":"Department of Environmental, Biological, Pharmaceutical Sciences and Technologies, University of Campania \u201cLuigi Vanvitelli\u201d , Via Vivaldi 43 , 81100\u00a0 Caserta ,","place":["Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marco","family":"Nardini","sequence":"additional","affiliation":[{"name":"Department of Biosciences, University of Milan , Via Celoria 26 , 20133\u00a0 Milano ,","place":["Italy"]},{"name":"Fondazione Romeo e Enrica Invernizzi and NOLIMITS, University of Milan , Via Celoria 26 , 20133\u00a0 Milan ,","place":["Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gaetano","family":"Malgieri","sequence":"additional","affiliation":[{"name":"Department of Environmental, Biological, Pharmaceutical Sciences and Technologies, University of Campania \u201cLuigi Vanvitelli\u201d , Via Vivaldi 43 , 81100\u00a0 Caserta 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Microbiol."},{"key":"2024122115430667000_B9","doi-asserted-by":"crossref","first-page":"511","DOI":"10.1016\/j.tim.2003.09.005","article-title":"H-NS in gram-negative bacteria: a family of multifaceted proteins","volume":"11","author":"Tendeng","year":"2003","journal-title":"Trends Microbiol."},{"key":"2024122115430667000_B10","doi-asserted-by":"crossref","first-page":"10184","DOI":"10.1093\/nar\/gkaa709","article-title":"Redefining the H-NS protein family: a diversity of specialized core and accessory forms exhibit hierarchical transcriptional network integration","volume":"48","author":"Fitzgerald","year":"2020","journal-title":"Nucleic Acids Res."},{"key":"2024122115430667000_B11","doi-asserted-by":"crossref","first-page":"7052","DOI":"10.1128\/JB.00733-08","article-title":"Lsr2 of Mycobacterium represents a novel class of H-NS-like proteins","volume":"190","author":"Gordon","year":"2008","journal-title":"J. Bacteriol."},{"key":"2024122115430667000_B12","doi-asserted-by":"crossref","first-page":"6211","DOI":"10.1128\/JB.00888-09","article-title":"The multifaceted proteins MvaT and MvaU, members of the H-NS family, control arginine metabolism, pyocyanin synthesis, and prophage activation in Pseudomonas aeruginosa PAO1","volume":"191","author":"Li","year":"2009","journal-title":"J. Bacteriol."},{"key":"2024122115430667000_B13","doi-asserted-by":"crossref","first-page":"916","DOI":"10.1111\/j.1365-2958.2010.07378.x","article-title":"High-order oligomerization is required for the function of the H-NS family member MvaT in Pseudomonas aeruginosa","volume":"78","author":"Castang","year":"2010","journal-title":"Mol. Microbiol."},{"key":"2024122115430667000_B14","doi-asserted-by":"crossref","first-page":"15728","DOI":"10.1073\/pnas.1006966107","article-title":"H-NS forms a superhelical protein scaffold for DNA condensation","volume":"107","author":"Arold","year":"2010","journal-title":"Proc. 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Microbiol."},{"key":"2024122115430667000_B31","doi-asserted-by":"crossref","first-page":"738","DOI":"10.1038\/s41396-021-01118-2","article-title":"The zinc-finger bearing xenogeneic silencer MucR in \u03b1-proteobacteria balances adaptation and regulatory integrity","volume":"16","author":"Jiao","year":"2022","journal-title":"ISME J."},{"key":"2024122115430667000_B32","doi-asserted-by":"crossref","first-page":"15536","DOI":"10.3390\/ijms232415536","article-title":"The Ros\/MucR zinc-finger protein family in bacteria: structure and functions","volume":"23","author":"Janczarek","year":"2022","journal-title":"Int. J. Mol. Sci."},{"key":"2024122115430667000_B33","doi-asserted-by":"crossref","first-page":"1040","DOI":"10.1128\/IAI.01097-12","article-title":"Diverse genetic regulon of the virulence-associated transcriptional regulator MucR in Brucella abortus 2308","volume":"81","author":"Caswell","year":"2013","journal-title":"Infect. 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Interact."},{"key":"2024122115430667000_B36","doi-asserted-by":"crossref","first-page":"5293","DOI":"10.1073\/pnas.95.9.5293","article-title":"Agrobacterium transcriptional regulator Ros is a prokaryotic zinc finger protein that regulates the plant oncogene ipt","volume":"95","author":"Chou","year":"1998","journal-title":"Proc. Natl Acad. Sci. U.S.A."},{"key":"2024122115430667000_B37","doi-asserted-by":"crossref","first-page":"2608","DOI":"10.1128\/jb.173.8.2608-2616.1991","article-title":"The virC and virD operons of the Agrobacterium Ti plasmid are regulated by the Ros chromosomal gene: analysis of the cloned Ros gene","volume":"173","author":"Cooley","year":"1991","journal-title":"J. Bacteriol."},{"key":"2024122115430667000_B38","doi-asserted-by":"crossref","first-page":"3486","DOI":"10.1128\/jb.175.11.3486-3490.1993","article-title":"Analysis of the Ros repressor of agrobacterium virC and virD operons: molecular intercommunication between plasmid and chromosomal genes","volume":"175","author":"D'Souza-Ault","year":"1993","journal-title":"J. Bacteriol."},{"key":"2024122115430667000_B39","doi-asserted-by":"crossref","first-page":"485","DOI":"10.1128\/JB.01129-10","article-title":"Complex regulation of symbiotic functions is coordinated by MucR and quorum sensing in Sinorhizobium meliloti","volume":"193","author":"Mueller","year":"2011","journal-title":"J. Bacteriol."},{"key":"2024122115430667000_B40","doi-asserted-by":"crossref","first-page":"1498","DOI":"10.1094\/MPMI-21-11-1498","article-title":"Sinorhizobium meliloti regulator MucR couples exopolysaccharide synthesis and motility","volume":"21","author":"Bahlawane","year":"2008","journal-title":"Mol. Plant Microbe. Interact."},{"key":"2024122115430667000_B41","doi-asserted-by":"crossref","first-page":"17341","DOI":"10.1073\/pnas.0706659104","article-title":"The prokaryotic Cys2His2 zinc-finger adopts a novel fold as revealed by the NMR structure of Agrobacterium tumefaciens Ros DNA-binding domain","volume":"104","author":"Malgieri","year":"2007","journal-title":"Proc. Natl Acad. Sci. U.S.A."},{"key":"2024122115430667000_B42","doi-asserted-by":"crossref","first-page":"6933","DOI":"10.1073\/pnas.0810003106","article-title":"The structural role of the zinc ion can be dispensable in prokaryotic zinc-finger domains","volume":"106","author":"Baglivo","year":"2009","journal-title":"Proc. Natl Acad. Sci. 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