{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,10]],"date-time":"2026-07-10T01:38:36Z","timestamp":1783647516609,"version":"3.55.0"},"reference-count":55,"publisher":"Oxford University Press (OUP)","issue":"1","license":[{"start":{"date-parts":[[2024,12,3]],"date-time":"2024-12-03T00:00:00Z","timestamp":1733184000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000024","name":"Canadian Institutes of Health Research","doi-asserted-by":"publisher","award":["FND167283"],"award-info":[{"award-number":["FND167283"]}],"id":[{"id":"10.13039\/501100000024","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,1,7]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>DNA transformation is key for phenotypic diversity and adaptation of Streptococcus pneumoniae including in the emergence of multidrug resistance (MDR). Under laboratory conditions, DNA transformation is facilitated by the artificial triggering of competence by the competence stimulating peptide (CSP). In ongoing DNA transformation work, we observed that exogenous CSP was dispensable depending on the combination of strains and culture media. Here, we carried out a chemogenomic screen to select for S. pneumoniae mutants capable of natural transformation in medium that normally would not sustain natural transformation. Our chemogenomic screen relied on chemical mutagenesis followed by selection of mutants with increased DNA transformation capacities. Sequencing the genome of these mutants revealed an abundance and diversity of mutated genes proven experimentally to increase natural transformation. A genome wide association study between MDR and sensitive clinical isolates revealed gene mutations associated with MDR, many of which intersected with those pinpointed by our chemogenomic screens and that were proven to increase natural transformation. S. pneumoniae has adopted DNA transformation as its lifestyle and can select for mutations facilitating DNA transformation.<\/jats:p>","DOI":"10.1093\/nar\/gkae1140","type":"journal-article","created":{"date-parts":[[2024,12,14]],"date-time":"2024-12-14T13:03:59Z","timestamp":1734181439000},"source":"Crossref","is-referenced-by-count":1,"title":["Point mutations in functionally diverse genes are associated with increased natural DNA transformation in multidrug resistant <i>Streptococcus pneumoniae<\/i>"],"prefix":"10.1093","volume":"53","author":[{"given":"Flora","family":"Peillard-Fiorente","sequence":"first","affiliation":[{"name":"Centre de Recherche en Infectiologie, Axe des Maladies Infectieuses et Immunitaires du CHU de Qu\u00e9bec and D\u00e9partement de Microbiologie, Infectiologie et Immunologie, Facult\u00e9 de M\u00e9decine, Universit\u00e9 Laval , 2707 Bd Laurier, Qu\u00e9bec , QC G1V 4G2,","place":["Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nguyen Phuong","family":"Pham","sequence":"additional","affiliation":[{"name":"Centre de Recherche en Infectiologie, Axe des Maladies Infectieuses et Immunitaires du CHU de Qu\u00e9bec and D\u00e9partement de Microbiologie, Infectiologie et Immunologie, Facult\u00e9 de M\u00e9decine, Universit\u00e9 Laval , 2707 Bd Laurier, Qu\u00e9bec , QC G1V 4G2,","place":["Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H\u00e9l\u00e8ne","family":"Gingras","sequence":"additional","affiliation":[{"name":"Centre de Recherche en Infectiologie, Axe des Maladies Infectieuses et Immunitaires du CHU de Qu\u00e9bec and D\u00e9partement de Microbiologie, Infectiologie et Immunologie, Facult\u00e9 de M\u00e9decine, Universit\u00e9 Laval , 2707 Bd Laurier, Qu\u00e9bec , QC G1V 4G2,","place":["Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chantal","family":"Godin","sequence":"additional","affiliation":[{"name":"Centre de Recherche en Infectiologie, Axe des Maladies Infectieuses et Immunitaires du CHU de Qu\u00e9bec and D\u00e9partement de Microbiologie, Infectiologie et Immunologie, Facult\u00e9 de M\u00e9decine, Universit\u00e9 Laval , 2707 Bd Laurier, Qu\u00e9bec , QC G1V 4G2,","place":["Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5172-6643","authenticated-orcid":false,"given":"Jie","family":"Feng","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences , 52, Sanlihe Rd, Xicheng District, Beijing\u00a0100045 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Philippe","family":"Leprohon","sequence":"additional","affiliation":[{"name":"Centre de Recherche en Infectiologie, Axe des Maladies Infectieuses et Immunitaires du CHU de Qu\u00e9bec and D\u00e9partement de Microbiologie, Infectiologie et Immunologie, Facult\u00e9 de M\u00e9decine, Universit\u00e9 Laval , 2707 Bd Laurier, Qu\u00e9bec , QC G1V 4G2,","place":["Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6743-9646","authenticated-orcid":false,"given":"Marc","family":"Ouellette","sequence":"additional","affiliation":[{"name":"Centre de Recherche en Infectiologie, Axe des Maladies Infectieuses et Immunitaires du CHU de Qu\u00e9bec and D\u00e9partement de Microbiologie, Infectiologie et Immunologie, Facult\u00e9 de M\u00e9decine, Universit\u00e9 Laval , 2707 Bd Laurier, Qu\u00e9bec , QC G1V 4G2,","place":["Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2024,12,3]]},"reference":[{"key":"2025011110015899000_B1","doi-asserted-by":"crossref","first-page":"893","DOI":"10.1016\/S0140-6736(09)61204-6","article-title":"Burden of disease caused by Streptococcus pneumoniae in children younger than 5 years: global estimates","volume":"374","author":"O\u2019Brien","year":"2009","journal-title":"Lancet"},{"key":"2025011110015899000_B2","doi-asserted-by":"crossref","first-page":"e744","DOI":"10.1016\/S2214-109X(18)30247-X","article-title":"Burden of Streptococcus pneumoniae and Haemophilus influenzae type b disease in children in the era of conjugate vaccines: global, regional, and national estimates for 2000\u201315","volume":"6","author":"Wahl","year":"2018","journal-title":"Lancet Glob. 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