{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T23:29:12Z","timestamp":1787009352700,"version":"build-2736575974"},"reference-count":44,"publisher":"Oxford University Press (OUP)","issue":"1","license":[{"start":{"date-parts":[[2024,12,9]],"date-time":"2024-12-09T00:00:00Z","timestamp":1733702400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003130","name":"Fonds Wetenschappelijk Onderzoek","doi-asserted-by":"publisher","award":["11H0323N"],"award-info":[{"award-number":["11H0323N"]}],"id":[{"id":"10.13039\/501100003130","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003130","name":"Fonds Wetenschappelijk Onderzoek","doi-asserted-by":"publisher","award":["11Q4T24N"],"award-info":[{"award-number":["11Q4T24N"]}],"id":[{"id":"10.13039\/501100003130","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003130","name":"Fonds Wetenschappelijk Onderzoek","doi-asserted-by":"publisher","award":["G0C5322N"],"award-info":[{"award-number":["G0C5322N"]}],"id":[{"id":"10.13039\/501100003130","id-type":"DOI","asserted-by":"publisher"}]},{"name":"KU Leuven Research Fund","award":["C14\/20\/087"],"award-info":[{"award-number":["C14\/20\/087"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,1,7]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>In order to increase our\u00a0command over genetically engineered bacterial populations in bioprocessing and therapy, synthetic regulatory circuitry needs to enable the temporal programming of a number of consecutive functional tasks without external interventions. In this context, we have engineered a genetic circuit encoding an autonomous but chemically tunable timer in Escherichia coli, based on the concept of a transcription factor cascade mediated by the cytoplasmic dilution of repressors. As proof-of-concept, we used this circuit to impose a time-resolved two-staged synthetic pathway composed of a production-followed-by-lysis program, via a single input. Moreover, via a recombinase step, this synchronous timer was further engineered into an asynchronous timer in which the generational distance of differentiating daughter cells spawning off from a stem-cell like mother cell becomes a predictable driver and proxy for timer dynamics. Using this asynchronous timer circuit, a temporally defined population heterogeneity can be programmed in bacterial populations.<\/jats:p>","DOI":"10.1093\/nar\/gkae1158","type":"journal-article","created":{"date-parts":[[2024,11,6]],"date-time":"2024-11-06T07:27:39Z","timestamp":1730878059000},"source":"Crossref","is-referenced-by-count":3,"title":["Tuning and functionalization of logic gates for time resolved programming of bacterial populations"],"prefix":"10.1093","volume":"53","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2933-8182","authenticated-orcid":false,"given":"Leonard E","family":"B\u00e4cker","sequence":"first","affiliation":[{"name":"Department of Microbial and Molecular Systems, Faculty of Bioscience Engineering, KU Leuven , Kasteelpark Arenberg 23\u2014bus 2457 , 3001 Leuven ,","place":["Belgium"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4670-8544","authenticated-orcid":false,"given":"Kevin","family":"Broux","sequence":"additional","affiliation":[{"name":"Department of Microbial and Molecular Systems, Faculty of Bioscience Engineering, KU Leuven , Kasteelpark Arenberg 23\u2014bus 2457 , 3001 Leuven ,","place":["Belgium"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Louise","family":"Weckx","sequence":"additional","affiliation":[{"name":"Department of Microbial and Molecular Systems, Faculty of Bioscience Engineering, KU Leuven , Kasteelpark Arenberg 23\u2014bus 2457 , 3001 Leuven ,","place":["Belgium"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sadhana","family":"Khanal","sequence":"additional","affiliation":[{"name":"Department of Microbial and Molecular Systems, Faculty of Bioscience Engineering, KU Leuven , Kasteelpark Arenberg 23\u2014bus 2457 , 3001 Leuven ,","place":["Belgium"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1897-2305","authenticated-orcid":false,"given":"Abram","family":"Aertsen","sequence":"additional","affiliation":[{"name":"Department of Microbial and Molecular Systems, Faculty of Bioscience Engineering, KU Leuven , Kasteelpark Arenberg 23\u2014bus 2457 , 3001 Leuven ,","place":["Belgium"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2024,12,9]]},"reference":[{"key":"2025011110032777400_B1","doi-asserted-by":"crossref","first-page":"e01524-14","DOI":"10.1128\/mBio.01524-14","article-title":"Metabolic engineering of microorganisms for the production of higher alcohols","volume":"5","author":"Choi","year":"2014","journal-title":"mBio"},{"key":"2025011110032777400_B2","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1186\/s12934-019-1090-4","article-title":"Metabolic engineering of microorganisms for production of aromatic compounds","volume":"18","author":"Huccetogullari","year":"2019","journal-title":"Microb. 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