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In bacteria, ProQ-like proteins have emerged as important RNA chaperones facilitating RNA stability and RNA duplex formation. In the major human pathogen Vibrio cholerae, post-transcriptional gene regulation is key for virulence, biofilm formation, and antibiotic resistance, yet the role of ProQ has not been studied. Here, we show that ProQ interacts with hundreds of transcripts in V. cholerae, including the highly abundant FlaX small RNA (sRNA). Global analyses of RNA duplex formation using RIL-Seq (RNA interaction by ligation and sequencing) revealed a vast network of ProQ-assisted interactions and identified a role for FlaX in motility regulation. Specifically, FlaX base-pairs with multiple sites on the flaB flagellin mRNA, preventing 30S ribosome binding and translation initiation. V. cholerae cells lacking flaX display impaired motility gene expression, altered flagella composition and reduced swimming in liquid environments. Our results provide a global view on ProQ-associated RNA duplex formation and pinpoint the mechanistic and phenotypic consequences associated with ProQ-associated sRNAs in V. cholerae.<\/jats:p>","DOI":"10.1093\/nar\/gkae1283","type":"journal-article","created":{"date-parts":[[2024,12,27]],"date-time":"2024-12-27T06:11:51Z","timestamp":1735279911000},"source":"Crossref","is-referenced-by-count":20,"title":["ProQ-associated small RNAs control motility in\n                    <i>Vibrio cholerae<\/i>"],"prefix":"10.1093","volume":"53","author":[{"given":"Rabea","family":"Ghandour","sequence":"first","affiliation":[{"name":"Friedrich Schiller University, Institute of Microbiology ,\u00a007743\u00a0 Jena ,","place":["Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel","family":"Devlitsarov","sequence":"additional","affiliation":[{"name":"Friedrich Schiller University, Institute of Microbiology ,\u00a007743\u00a0 Jena ,","place":["Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Phillip","family":"Popp","sequence":"additional","affiliation":[{"name":"Humboldt-Universit\u00e4t zu Berlin, Institute for Biology , 10115\u00a0 Berlin ,","place":["Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sahar","family":"Melamed","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Molecular Genetics, Institute for Medical Research Israel-Canada, Faculty of Medicine, The Hebrew University of Jerusalem , Jerusalem ,","place":["Israel"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michaela","family":"Huber","sequence":"additional","affiliation":[{"name":"Friedrich Schiller University, Institute of Microbiology ,\u00a007743\u00a0 Jena ,","place":["Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8458-1938","authenticated-orcid":false,"given":"Malte","family":"Siemers","sequence":"additional","affiliation":[{"name":"Friedrich Schiller University, Institute of Microbiology ,\u00a007743\u00a0 Jena ,","place":["Germany"]},{"name":"Microverse Cluster, Friedrich Schiller University Jena , 07743\u00a0 Jena ,","place":["Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas","family":"Kr\u00fcger","sequence":"additional","affiliation":[{"name":"Department of Molecular and Applied Microbiology, Leibniz Institute for Natural Product Research and Infection Biology (Leibniz-HKI) , 07745\u00a0 Jena ,","place":["Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Olaf","family":"Kniemeyer","sequence":"additional","affiliation":[{"name":"LMU Munich Biocenter, Ludwig-Maximilian-University of Munich , 82152\u00a0 Munich ,","place":["Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8414-8317","authenticated-orcid":false,"given":"Andreas","family":"Klingl","sequence":"additional","affiliation":[{"name":"LMU Munich Biocenter, Ludwig-Maximilian-University of Munich , 82152\u00a0 Munich ,","place":["Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8814-4193","authenticated-orcid":false,"given":"Axel\u00a0A","family":"Brakhage","sequence":"additional","affiliation":[{"name":"Friedrich Schiller University, Institute of Microbiology ,\u00a007743\u00a0 Jena ,","place":["Germany"]},{"name":"Microverse Cluster, Friedrich Schiller University Jena , 07743\u00a0 Jena ,","place":["Germany"]},{"name":"Department of Molecular and Applied Microbiology, Leibniz Institute for Natural Product Research and Infection Biology (Leibniz-HKI) , 07745\u00a0 Jena ,","place":["Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marc","family":"Erhardt","sequence":"additional","affiliation":[{"name":"Humboldt-Universit\u00e4t zu Berlin, Institute for Biology , 10115\u00a0 Berlin ,","place":["Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5560-9804","authenticated-orcid":false,"given":"Kai","family":"Papenfort","sequence":"additional","affiliation":[{"name":"Friedrich Schiller University, Institute of Microbiology ,\u00a007743\u00a0 Jena ,","place":["Germany"]},{"name":"Microverse Cluster, Friedrich Schiller University Jena , 07743\u00a0 Jena 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