{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,19]],"date-time":"2026-05-19T05:53:55Z","timestamp":1779170035893,"version":"3.51.4"},"reference-count":52,"publisher":"Oxford University Press (OUP)","issue":"12","license":[{"start":{"date-parts":[[2024,6,6]],"date-time":"2024-06-06T00:00:00Z","timestamp":1717632000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/100020595","name":"National Science and Technology Council","doi-asserted-by":"publisher","award":["NSTC112-2311-B008-001"],"award-info":[{"award-number":["NSTC112-2311-B008-001"]}],"id":[{"id":"10.13039\/100020595","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100011912","name":"Taipei Veterans General Hospital","doi-asserted-by":"publisher","award":["VGHUST113-G4-1-1"],"award-info":[{"award-number":["VGHUST113-G4-1-1"]}],"id":[{"id":"10.13039\/501100011912","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,7,8]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Prolyl-tRNA synthetases (ProRSs) are unique among aminoacyl-tRNA synthetases (aaRSs) in having two distinct structural architectures across different organisms: prokaryote-like (P-type) and eukaryote\/archaeon-like (E-type). Interestingly, Bacillus thuringiensis harbors both types, with P-type (BtProRS1) and E-type ProRS (BtProRS2) coexisting. Despite their differences, both enzymes are constitutively expressed and functional in vivo. Similar to BtProRS1, BtProRS2 selectively charges the P-type tRNAPro and displays higher halofuginone tolerance than canonical E-type ProRS. However, these two isozymes recognize the primary identity elements of the P-type tRNAPro\u2015G72 and A73 in the acceptor stem\u2015through distinct mechanisms. Moreover, BtProRS2 exhibits significantly higher tolerance to stresses (such as heat, hydrogen peroxide, and dithiothreitol) than BtProRS1 does. This study underscores how an E-type ProRS adapts to a P-type tRNAPro and how it may contribute to the bacterium's survival under stress conditions.<\/jats:p>","DOI":"10.1093\/nar\/gkae483","type":"journal-article","created":{"date-parts":[[2024,6,6]],"date-time":"2024-06-06T17:49:54Z","timestamp":1717696194000},"page":"7158-7170","source":"Crossref","is-referenced-by-count":4,"title":["Adaptation of a eukaryote-like ProRS to a prokaryote-like tRNAPro"],"prefix":"10.1093","volume":"52","author":[{"given":"Indira Rizqita","family":"Ivanesthi","sequence":"first","affiliation":[{"name":"Department of Life Sciences, National Central University , Zhongli District, Taoyuan 320317, Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Emi","family":"Latifah","sequence":"additional","affiliation":[{"name":"Department of Life Sciences, National Central University , Zhongli District, Taoyuan 320317, Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luqman Fikri","family":"Amrullah","sequence":"additional","affiliation":[{"name":"Department of Life Sciences, National Central University , Zhongli District, Taoyuan 320317, Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yi-Kuan","family":"Tseng","sequence":"additional","affiliation":[{"name":"Graduate Institute of Statistics, National Central University , Zhongli District, Taoyuan 320317, Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5275-9022","authenticated-orcid":false,"given":"Tsung-Hsien","family":"Chuang","sequence":"additional","affiliation":[{"name":"Immunology Research Center, National Health Research Institutes , Zhunan Town, Miaoli 35053, Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hung-Chuan","family":"Pan","sequence":"additional","affiliation":[{"name":"Department of Neurosurgery, Taichung Veterans General Hospital , Taichung 407219, Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chih-Shiang","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Life Sciences, National Central University , Zhongli District, Taoyuan 320317, Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shih-Yang","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Life Sciences, National Central University , Zhongli District, Taoyuan 320317, Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7104-1307","authenticated-orcid":false,"given":"Chien-Chia","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Life Sciences, National Central University , Zhongli District, Taoyuan 320317, Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2024,6,6]]},"reference":[{"key":"2024070813445622800_B1","doi-asserted-by":"crossref","first-page":"715","DOI":"10.1146\/annurev.bi.62.070193.003435","article-title":"Cognition, mechanism, and evolutionary relationships in aminoacyl-tRNA synthetases","volume":"62","author":"Carter","year":"1993","journal-title":"Annu. 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