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In this study, we identified a new anti-CRISPR gene (acrIE10) on a conjugative plasmid with self-target sequence in K. pneumoniae with type I-E* CRISPR-Cas system. AcrIE10 interacts with the Cas7* subunit of K. pneumoniae I-E* CRISPR-Cas system. The crystal structure of the AcrIE10-KpCas7* complex suggests that AcrIE10 suppresses the I-E* CRISPR-Cas by binding directly to Cas7 to prevent its hexamerization, thereby preventing the surveillance complex assembly and crRNA loading. Bioinformatic and functional analyses revealed that AcrIE10 is functionally widespread across diverse species. Our study reports a novel anti-CRISPR and highlights its potential role in spreading resistance and virulence among pathogens.<\/jats:p>","DOI":"10.1093\/nar\/gkae516","type":"journal-article","created":{"date-parts":[[2024,6,18]],"date-time":"2024-06-18T10:59:22Z","timestamp":1718708362000},"page":"8370-8384","source":"Crossref","is-referenced-by-count":18,"title":["A new anti-CRISPR gene promotes the spread of drug-resistance plasmids in <i>Klebsiella pneumoniae<\/i>"],"prefix":"10.1093","volume":"52","author":[{"given":"Chunyu","family":"Jiang","sequence":"first","affiliation":[{"name":"Department of Microbiology and Immunology, Shanghai Jiao Tong University School of Medicine , Shanghai 200025 , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chengzhi","family":"Yu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Synthetic Biology, State Key Laboratory of Plant Design, CAS Center for Excellence in Molecular Plant Sciences, Shanghai Institute 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