{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T03:04:36Z","timestamp":1791428676318,"version":"4.3.3"},"reference-count":55,"publisher":"Oxford University Press (OUP)","issue":"14","license":[{"start":{"date-parts":[[2024,6,25]],"date-time":"2024-06-25T00:00:00Z","timestamp":1719273600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Agence Nationale pour la Recherche","award":["ANR-11-INBS-0011"],"award-info":[{"award-number":["ANR-11-INBS-0011"]}]},{"name":"Agence Nationale pour la Recherche","award":["ANR-22-PEBI-0011"],"award-info":[{"award-number":["ANR-22-PEBI-0011"]}]},{"name":"Agence Nationale pour la Recherche","award":["ANR-20-CE18-0012-02"],"award-info":[{"award-number":["ANR-20-CE18-0012-02"]}]},{"name":"Agence Nationale pour la Recherche","award":["CEA"],"award-info":[{"award-number":["CEA"]}]},{"name":"Agence Nationale pour la Recherche","award":["France Parkinson"],"award-info":[{"award-number":["France Parkinson"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,8,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Synucleinopathies, including dementia with Lewy bodies (DLB), Parkinson's disease (PD), and multiple system atrophy (MSA), are characterized by the presence of \u03b1-synuclein (\u03b1-syn) aggregates in the central nervous system. Recent evidence suggests that the heterogeneity of synucleinopathies may be partly explained by the fact that patients may have different \u03b1-syn fibrillar polymorphs with structural differences. In this study, we identify nuclease resistant 2\u2032fluoro-pyrimidine RNA aptamers that can differentially bind to structurally distinct \u03b1-syn fibrillar polymorphs. Moreover, we introduce a method, AptaFOOT-Seq, designed to rapidly assess the affinity of a mixture of these aptamers for different \u03b1-SYN fibrillar polymorphs using next-generation sequencing. Our findings reveal that the binding behavior of aptamers can be very different when they are tested separately or in the presence of other aptamers. In this case, competition and cooperation can occur, providing a higher level of information, which can be exploited to obtain specific \u2018footprints\u2019 for different \u03b1-Syn fibrillar polymorphs. Notably, these footprints can distinguish polymorphs obtained from patients with PD, DLB or MSA. This result suggests that aptaFOOT-Seq could be used for the detection of misfolded or abnormal protein conformations to improve the diagnosis of synucleinopathies.<\/jats:p>","DOI":"10.1093\/nar\/gkae544","type":"journal-article","created":{"date-parts":[[2024,6,25]],"date-time":"2024-06-25T19:30:53Z","timestamp":1719343853000},"page":"8072-8085","source":"Crossref","is-referenced-by-count":6,"title":["Aptamer binding footprints discriminate \u03b1-synuclein fibrillar polymorphs from different synucleinopathies"],"prefix":"10.1093","volume":"52","author":[{"given":"Alix","family":"Bouvier-M\u00fcller","sequence":"first","affiliation":[{"name":"CEA, DRF, Institut of biology JACOB, Molecular Imaging Research Center (MIRCen) , Fontenay aux roses 92335 , France"},{"name":"CNRS UMR 9199, Laboratoire des Maladies Neurod\u00e9g\u00e9n\u00e9ratives , Fontenay aux roses 92335 , France"},{"name":"Universit\u00e9 Paris-Saclay , Fontenay aux roses 92335 , France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Deborah","family":"Fourmy","sequence":"additional","affiliation":[{"name":"CEA, DRF, Institut of biology JACOB, Molecular Imaging Research Center (MIRCen) , Fontenay aux roses 92335 , France"},{"name":"CNRS UMR 9199, Laboratoire des Maladies Neurod\u00e9g\u00e9n\u00e9ratives , Fontenay aux roses 92335 , France"},{"name":"Universit\u00e9 Paris-Saclay , Fontenay aux roses 92335 , France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexis","family":"Fenyi","sequence":"additional","affiliation":[{"name":"CEA, DRF, Institut of biology JACOB, Molecular Imaging Research Center (MIRCen) , Fontenay aux roses 92335 , France"},{"name":"CNRS UMR 9199, Laboratoire des Maladies Neurod\u00e9g\u00e9n\u00e9ratives , Fontenay aux roses 92335 , France"},{"name":"Universit\u00e9 Paris-Saclay , Fontenay aux roses 92335 , France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luc","family":"Bousset","sequence":"additional","affiliation":[{"name":"CEA, DRF, Institut of biology JACOB, Molecular Imaging Research Center (MIRCen) , Fontenay aux roses 92335 , France"},{"name":"CNRS UMR 9199, Laboratoire des Maladies Neurod\u00e9g\u00e9n\u00e9ratives , Fontenay aux roses 92335 , France"},{"name":"Universit\u00e9 Paris-Saclay , Fontenay aux roses 92335 , France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ronald","family":"Melki","sequence":"additional","affiliation":[{"name":"CEA, DRF, Institut of biology JACOB, Molecular Imaging Research Center (MIRCen) , Fontenay aux roses 92335 , France"},{"name":"CNRS UMR 9199, Laboratoire des Maladies Neurod\u00e9g\u00e9n\u00e9ratives , Fontenay aux roses 92335 , France"},{"name":"Universit\u00e9 Paris-Saclay , Fontenay aux roses 92335 , France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8595-0050","authenticated-orcid":false,"given":"Fr\u00e9d\u00e9ric","family":"Ducong\u00e9","sequence":"additional","affiliation":[{"name":"CEA, DRF, Institut of biology JACOB, Molecular Imaging Research Center (MIRCen) , Fontenay aux roses 92335 , France"},{"name":"CNRS UMR 9199, Laboratoire des Maladies Neurod\u00e9g\u00e9n\u00e9ratives , Fontenay aux roses 92335 , France"},{"name":"Universit\u00e9 Paris-Saclay , Fontenay aux roses 92335 , France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2024,6,25]]},"reference":[{"key":"2024081123584856900_B1","first-page":"S51","article-title":"The synucleinopathies: twenty years on","volume":"7","author":"Goedert","year":"2017","journal-title":"J. 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