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Challenging sequences include proline-rich motifs as well as highly positively and negatively charged amino acid stretches. Members of the F subfamily of ABC ATPases (ABCFs) have been long hypothesised to promote translation of such problematic motifs. In this study we have applied genetics and reporter-based assays to characterise the four housekeeping ABCF ATPases of Bacillus subtilis: YdiF, YfmM, YfmR\/Uup and YkpA\/YbiT. We show that YfmR cooperates with the translation factor EF-P that promotes translation of Pro-rich motifs. Simultaneous loss of both YfmR and EF-P results in a dramatic growth defect. Surprisingly, this growth defect can be largely suppressed though overexpression of an EF-P variant lacking the otherwise crucial 5-amino-pentanolylated residue K32. Using in vivo reporter assays, we show that overexpression of YfmR can alleviate ribosomal stalling on Asp-Pro motifs. Finally, we demonstrate that YkpA\/YbiT promotes translation of positively and negatively charged motifs but is inactive in resolving ribosomal stalls on proline-rich stretches. Collectively, our results provide insights into the function of ABCF translation factors in modulating protein synthesis in B. subtilis.<\/jats:p>","DOI":"10.1093\/nar\/gkae556","type":"journal-article","created":{"date-parts":[[2024,6,29]],"date-time":"2024-06-29T04:01:06Z","timestamp":1719633666000},"page":"9854-9866","source":"Crossref","is-referenced-by-count":30,"title":["Resolution of ribosomal stalling by EF-P and ABCF ATPases YfmR and YkpA\/YbiT"],"prefix":"10.1093","volume":"52","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2034-5666","authenticated-orcid":false,"given":"Hiraku","family":"Takada","sequence":"first","affiliation":[{"name":"Faculty of Life Sciences and Institute for Protein Dynamics, Kyoto Sangyo University , Kamigamo, Motoyama, Kita-ku , Kyoto\u00a0 603-8555 , Japan"},{"name":"Department of Biotechnology, Toyama Prefectural University , 5180 Kurokawa , Imizu , Toyama\u00a0 939-0398 , Japan"},{"name":"Department of Experimental Medical Science, Lund University , 221 00\u00a0 Lund , Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Keigo","family":"Fujiwara","sequence":"additional","affiliation":[{"name":"Faculty of Life Sciences and Institute for Protein Dynamics, Kyoto Sangyo University , Kamigamo, Motoyama, Kita-ku , Kyoto\u00a0 603-8555 , Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gemma\u00a0C","family":"Atkinson","sequence":"additional","affiliation":[{"name":"Department of Experimental Medical Science, Lund University , 221 00\u00a0 Lund , Sweden"},{"name":"Virus Centre, Lund University , Lund , Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3485-3477","authenticated-orcid":false,"given":"Shinobu","family":"Chiba","sequence":"additional","affiliation":[{"name":"Faculty of Life Sciences and Institute for Protein Dynamics, Kyoto Sangyo University , Kamigamo, Motoyama, Kita-ku , Kyoto\u00a0 603-8555 , Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2389-5057","authenticated-orcid":false,"given":"Vasili","family":"Hauryliuk","sequence":"additional","affiliation":[{"name":"Department of Experimental Medical Science, Lund University , 221 00\u00a0 Lund , Sweden"},{"name":"Virus Centre, Lund University , Lund , Sweden"},{"name":"University of Tartu, Institute of Technology , 50411\u00a0 Tartu , Estonia"},{"name":"Science for Life Laboratory , Lund , Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2024,6,29]]},"reference":[{"key":"2024090903590997900_B1","doi-asserted-by":"crossref","first-page":"78","DOI":"10.1186\/s12864-015-1289-7","article-title":"The evolutionary and functional diversity of classical and lesser-known cytoplasmic and organellar translational GTPases across the tree of life","volume":"16","author":"Atkinson","year":"2015","journal-title":"BMC Genomics"},{"key":"2024090903590997900_B2","doi-asserted-by":"crossref","first-page":"816398","DOI":"10.3389\/fmolb.2021.816398","article-title":"Functions and regulation of translation elongation factors","volume":"8","author":"Xu","year":"2021","journal-title":"Front Mol. 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