{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T02:14:28Z","timestamp":1791512068575,"version":"4.3.4"},"reference-count":58,"publisher":"Oxford University Press (OUP)","issue":"17","license":[{"start":{"date-parts":[[2024,8,16]],"date-time":"2024-08-16T00:00:00Z","timestamp":1723766400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000060","name":"National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["R01AI127652"],"award-info":[{"award-number":["R01AI127652"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]},{"name":"National Institute of Health National Research Service Award Trainee Fellowship","award":["5T32 CH132022"],"award-info":[{"award-number":["5T32 CH132022"]}]},{"DOI":"10.13039\/100000060","name":"National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]},{"name":"National Institute of Health"},{"name":"Burroughs Wellcome"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,9,23]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>ICP1, a lytic bacteriophage of Vibrio cholerae, is parasitized by phage satellites, PLEs, which hijack ICP1 proteins for their own horizontal spread. PLEs' dependence on ICP1\u2019s DNA replication machinery\u00a0and virion components results in inhibition of ICP1\u2019s lifecycle. PLEs\u00a0are expected to depend on ICP1 factors for genome packaging, but the mechanism(s) PLEs use to inhibit ICP1 genome packaging is currently unknown. Here, we identify and characterize Gpi, PLE\u2019s indiscriminate genome packaging inhibitor. Gpi binds to ICP1\u2019s large terminase (TerL), the packaging motor, and blocks genome packaging. To overcome Gpi's negative effect on TerL, a component PLE also requires, PLE uses two genome packaging specifiers, GpsA and GpsB, that specifically allow packaging of PLE genomes. Surprisingly, PLE also uses mimicry of ICP1\u2019s pac site as a backup strategy to ensure genome packaging. PLE\u2019s pac site mimicry, however, is only sufficient if PLE can inhibit ICP1 at other stages of its lifecycle, suggesting an advantage to maintaining Gpi, GpsA\u00a0and GpsB. Collectively, these results provide mechanistic insights into another stage of ICP1\u2019s lifecycle that is inhibited by PLE, which is currently the most inhibitory of the documented phage satellites. More broadly, Gpi represents the first satellite-encoded inhibitor of a phage TerL.<\/jats:p>","DOI":"10.1093\/nar\/gkae675","type":"journal-article","created":{"date-parts":[[2024,7,26]],"date-time":"2024-07-26T21:59:46Z","timestamp":1722031186000},"page":"10431-10446","source":"Crossref","is-referenced-by-count":14,"title":["A phage satellite manipulates the viral DNA packaging motor to inhibit phage and promote satellite spread"],"prefix":"10.1093","volume":"52","author":[{"given":"Caroline\u00a0M","family":"Boyd","sequence":"first","affiliation":[{"name":"Plant and Microbial Biology, University of California - Berkeley , Berkeley , CA\u00a0 94720 , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0139-1600","authenticated-orcid":false,"given":"Kimberley\u00a0D","family":"Seed","sequence":"additional","affiliation":[{"name":"Plant and Microbial Biology, University of California - Berkeley , Berkeley , CA\u00a0 94720 , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2024,8,16]]},"reference":[{"key":"2024092307452192000_B1","doi-asserted-by":"crossref","first-page":"2759","DOI":"10.1093\/nar\/gkad123","article-title":"Identification and characterization of thousands of bacteriophage satellites across bacteria","volume":"51","author":"de\u00a0Sousa","year":"2023","journal-title":"Nucleic Acids Res."},{"key":"2024092307452192000_B2","first-page":"369","article-title":"Viral genome packaging machines: structure and enzymology","volume-title":"The Enzymes","author":"Catalano","year":"2021"},{"key":"2024092307452192000_B3","doi-asserted-by":"crossref","first-page":"351","DOI":"10.1146\/annurev-virology-100114-055212","article-title":"Mechanisms of DNA packaging by large double-stranded DNA viruses","volume":"2","author":"Rao","year":"2015","journal-title":"Annu. 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