{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T19:52:28Z","timestamp":1790279548710,"version":"4.1.0"},"reference-count":59,"publisher":"Oxford University Press (OUP)","issue":"17","license":[{"start":{"date-parts":[[2024,8,27]],"date-time":"2024-08-27T00:00:00Z","timestamp":1724716800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Key R&D Program of China","doi-asserted-by":"publisher","award":["2021YFC2300200"],"award-info":[{"award-number":["2021YFC2300200"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012166","name":"National Key R&D Program of China","doi-asserted-by":"publisher","award":["2021YFC2300700"],"award-info":[{"award-number":["2021YFC2300700"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81871658"],"award-info":[{"award-number":["81871658"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32192452"],"award-info":[{"award-number":["32192452"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32100119"],"award-info":[{"award-number":["32100119"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32100129"],"award-info":[{"award-number":["32100129"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,9,23]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>African swine fever virus (ASFV) is one of the most important causative agents of animal diseases and can cause highly fatal diseases in swine. ASFV DNA polymerase (DNAPol) is responsible for genome replication and highly conserved in all viral genotypes showing an ideal target for drug development. Here, we systematically determined the structures of ASFV DNAPol in apo, replicating and editing states. Structural analysis revealed that ASFV DNAPol had a classical right-handed structure and showed the highest similarity to the structure of human polymerase delta. Intriguingly, ASFV DNAPol has a much longer fingers subdomain, and the thumb and palm subdomain form a unique interaction that has never been seen. Mutagenesis work revealed that the loss of this unique interaction decreased the enzymatic activity. We also found that the \u03b2-hairpin of ASFV DNAPol is located below the template strand in the editing state, which is different from the editing structures of other known B family DNAPols with the \u03b2-hairpin above the template strand. It suggests that B family DNAPols have evolved two ways to facilitate the dsDNA unwinding during the transition from replicating into editing state. These findings figured out the working mechanism of ASFV DNAPol and will provide a critical structural basis for the development of antiviral drugs.<\/jats:p>","DOI":"10.1093\/nar\/gkae739","type":"journal-article","created":{"date-parts":[[2024,8,27]],"date-time":"2024-08-27T10:59:03Z","timestamp":1724756343000},"page":"10717-10729","source":"Crossref","is-referenced-by-count":8,"title":["Cryo-EM structure of DNA polymerase of African swine fever virus"],"prefix":"10.1093","volume":"52","author":[{"given":"Lu","family":"Kuai","sequence":"first","affiliation":[{"name":"CAS Key Laboratory of Pathogen Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences , Beijing 100101 , China"},{"name":"Medical School, University of Chinese Academy of Sciences , Beijing 100049 , China"},{"name":"Beijing Life Science Academy , Beijing 102209 , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junqing","family":"Sun","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Shanxi Agricultural University , Jinzhong , 030801 , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qi","family":"Peng","sequence":"additional","affiliation":[{"name":"CAS Key Laboratory of Pathogen Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences , Beijing 100101 , China"},{"name":"Beijing Life Science Academy , Beijing 102209 , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xuejin","family":"Zhao","sequence":"additional","affiliation":[{"name":"CAS Key Laboratory of Pathogen Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences , Beijing 100101 , China"},{"name":"Beijing Life Science Academy , Beijing 102209 , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bin","family":"Yuan","sequence":"additional","affiliation":[{"name":"CAS Key Laboratory of Pathogen Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences , Beijing 100101 , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sheng","family":"Liu","sequence":"additional","affiliation":[{"name":"Shenzhen Children's Hospital , Shenzhen 518038 , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuhai","family":"Bi","sequence":"additional","affiliation":[{"name":"CAS Key Laboratory of Pathogen Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences , Beijing 100101 , China"},{"name":"Medical School, University of Chinese Academy of Sciences , Beijing 100049 , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3053-2687","authenticated-orcid":false,"given":"Yi","family":"Shi","sequence":"additional","affiliation":[{"name":"CAS Key Laboratory of Pathogen Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences , Beijing 100101 , China"},{"name":"Medical School, University of Chinese Academy of Sciences , Beijing 100049 , China"},{"name":"Beijing Life Science Academy , Beijing 102209 , China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2024,8,27]]},"reference":[{"key":"2024092307420791300_B1","doi-asserted-by":"crossref","first-page":"197667","DOI":"10.1016\/j.virusres.2019.197667","article-title":"African swine fever virus replication events and cell nucleus: new insights and perspectives","volume":"270","author":"Simoes","year":"2019","journal-title":"Virus Res."},{"key":"2024092307420791300_B2","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1177\/0300985809354350","article-title":"Agricultural diseases on the move early in the third millennium","volume":"47","author":"Arzt","year":"2010","journal-title":"Vet. Pathol."},{"key":"2024092307420791300_B3","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1016\/j.tvjl.2017.12.025","article-title":"African swine fever: a re-emerging viral disease threatening the global pig industry","volume":"233","author":"S\u00e1nchez-Cord\u00f3n","year":"2018","journal-title":"Vet. J."},{"key":"2024092307420791300_B4","doi-asserted-by":"crossref","first-page":"1482","DOI":"10.1111\/tbed.12989","article-title":"Emergence of African swine fever in China, 2018","volume":"65","author":"Zhou","year":"2018","journal-title":"Transbound. Emerg. Dis."},{"key":"2024092307420791300_B5","doi-asserted-by":"crossref","first-page":"2722","DOI":"10.1111\/tbed.14039","article-title":"African Swine Fever spread across Asia, 2018-2019","volume":"68","author":"Mighell","year":"2021","journal-title":"Transbound. Emerg. Dis."},{"key":"2024092307420791300_B6","doi-asserted-by":"crossref","first-page":"734236","DOI":"10.3389\/fvets.2021.734236","article-title":"Beyond numbers: determining the socioeconomic and livelihood impacts of African Swine Fever and its control in the Philippines","volume":"8","author":"Cooper","year":"2022","journal-title":"Front. Vet. Sci."},{"key":"2024092307420791300_B7","doi-asserted-by":"crossref","first-page":"155","DOI":"10.1016\/j.actatropica.2016.11.020","article-title":"The emergence of arthropod-borne viral diseases: a global prospective on dengue, chikungunya and zika fevers","volume":"166","author":"Mayer","year":"2017","journal-title":"Acta Trop."},{"key":"2024092307420791300_B8","doi-asserted-by":"crossref","first-page":"18","DOI":"10.1016\/j.hlife.2023.10.006","article-title":"Crystal structures of RNA-dependent RNA polymerases from Jingmen tick virus and Alongshan virus","volume":"2","author":"Liu","year":"2024","journal-title":"Hlife"},{"key":"2024092307420791300_B9","doi-asserted-by":"crossref","first-page":"836","DOI":"10.1016\/j.chom.2019.11.004","article-title":"Cryo-EM structure of the African Swine Fever Virus","volume":"26","author":"Liu","year":"2019","journal-title":"Cell Host Microbe"},{"key":"2024092307420791300_B10","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1074\/jbc.AC119.011196","article-title":"The cryo-EM structure of African swine fever virus unravels a unique architecture comprising two icosahedral protein capsids and two lipoprotein membranes","volume":"295","author":"Andr\u00e9s","year":"2020","journal-title":"J. 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Rep."},{"key":"2024092307420791300_B25","doi-asserted-by":"crossref","first-page":"e01058-17","DOI":"10.1128\/JVI.01058-17","article-title":"BA71\u0394CD2: a new recombinant live attenuated African Swine fever virus with cross-protective capabilities","volume":"91","author":"Monteagudo","year":"2017","journal-title":"J. Virol."},{"key":"2024092307420791300_B26","doi-asserted-by":"crossref","first-page":"8","DOI":"10.1016\/j.virusres.2016.05.014","article-title":"African swine fever virus Georgia isolate harboring deletions of 9GL and MGF360\/505 genes is highly attenuated in swine but does not confer protection against parental virus challenge","volume":"221","author":"O\u2019Donnell","year":"2016","journal-title":"Virus Res."},{"key":"2024092307420791300_B27","doi-asserted-by":"crossref","first-page":"387","DOI":"10.1038\/s41467-019-08296-w","article-title":"Structure of the error-prone DNA ligase of African swine fever virus identifies critical active site residues","volume":"10","author":"Chen","year":"2019","journal-title":"Nat. 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Virol."},{"key":"2024092307420791300_B31","doi-asserted-by":"crossref","first-page":"9475","DOI":"10.1093\/nar\/gkad677","article-title":"Structures and implications of the C962R protein of African swine fever virus","volume":"51","author":"Shao","year":"2023","journal-title":"Nucleic Acids Res."},{"key":"2024092307420791300_B32","doi-asserted-by":"crossref","first-page":"e01228-23","DOI":"10.1128\/mbio.01228-23","article-title":"Cryo-EM structures of African swine fever virus topoisomerase","volume":"14","author":"Zhao","year":"2023","journal-title":"mBio"},{"key":"2024092307420791300_B33","doi-asserted-by":"crossref","first-page":"e1002599","DOI":"10.1371\/journal.pbio.1002599","article-title":"Unique 5\u2032-P recognition and basis for dG: dGTP misincorporation of ASFV DNA polymerase X","volume":"15","author":"Chen","year":"2017","journal-title":"PLoS Biol."},{"key":"2024092307420791300_B34","doi-asserted-by":"crossref","first-page":"603","DOI":"10.1038\/s41586-023-06608-1","article-title":"Molecular mechanism of de novo replication by the Ebola virus polymerase","volume":"622","author":"Peng","year":"2023","journal-title":"Nature"},{"key":"2024092307420791300_B35","first-page":"100080","article-title":"Structural basis of SARS-CoV-2 polymerase inhibition by Favipiravir","volume":"2","author":"Peng","year":"2021","journal-title":"Innovation (Camb)"},{"key":"2024092307420791300_B36","doi-asserted-by":"crossref","first-page":"100","DOI":"10.1126\/science.ade6360","article-title":"Structure of monkeypox virus DNA polymerase holoenzyme","volume":"379","author":"Peng","year":"2023","journal-title":"Science"},{"key":"2024092307420791300_B37","doi-asserted-by":"crossref","first-page":"331","DOI":"10.1038\/nmeth.4193","article-title":"MotionCor2: anisotropic correction of beam-induced motion for improved cryo-electron microscopy","volume":"14","author":"Zheng","year":"2017","journal-title":"Nat. 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Biol."},{"key":"2024092307420791300_B41","doi-asserted-by":"crossref","first-page":"583","DOI":"10.1038\/s41586-021-03819-2","article-title":"Highly accurate protein structure prediction with AlphaFold","volume":"596","author":"Jumper","year":"2021","journal-title":"Nature"},{"key":"2024092307420791300_B42","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1107\/S0907444909052925","article-title":"PHENIX: a comprehensive Python-based system for macromolecular structure solution","volume":"66","author":"Adams","year":"2010","journal-title":"Acta. Crystallogr. D Biol. Crystallogr."},{"key":"2024092307420791300_B43","doi-asserted-by":"crossref","first-page":"2126","DOI":"10.1107\/S0907444904019158","article-title":"Coot: model-building tools for molecular graphics","volume":"60","author":"Emsley","year":"2004","journal-title":"Acta. Crystallogr. D Biol. Crystallogr."},{"key":"2024092307420791300_B44","doi-asserted-by":"crossref","first-page":"12","DOI":"10.1107\/S0907444909042073","article-title":"MolProbity: all-atom structure validation for macromolecular crystallography","volume":"66","author":"Chen","year":"2010","journal-title":"Acta. Crystallogr. D Biol. Crystallogr."},{"key":"2024092307420791300_B45","doi-asserted-by":"crossref","first-page":"14","DOI":"10.1002\/pro.3235","article-title":"UCSF ChimeraX: meeting modern challenges in visualization and analysis","volume":"27","author":"Goddard","year":"2018","journal-title":"Protein Sci."},{"key":"2024092307420791300_B46","doi-asserted-by":"crossref","first-page":"1109","DOI":"10.1038\/s41467-020-14898-6","article-title":"Structure of the processive human pol \u03b4 holoenzyme","volume":"11","author":"Lancey","year":"2020","journal-title":"Nat. Commun."},{"key":"2024092307420791300_B47","doi-asserted-by":"crossref","first-page":"979","DOI":"10.1038\/nsmb.1663","article-title":"Structural basis of high-fidelity DNA synthesis by yeast DNA polymerase \u03b4","volume":"16","author":"Swan","year":"2009","journal-title":"Nat. Struct. Mol. Biol."},{"key":"2024092307420791300_B48","doi-asserted-by":"crossref","first-page":"304","DOI":"10.1016\/j.semcancer.2010.10.003","article-title":"Mutator phenotypes due to DNA replication infidelity","volume":"20","author":"Arana","year":"2010","journal-title":"Semin. Cancer Biol."},{"key":"2024092307420791300_B49","doi-asserted-by":"crossref","first-page":"43770","DOI":"10.1074\/jbc.M306893200","article-title":"Unique error signature of the four-subunit yeast DNA polymerase \u03f5","volume":"278","author":"Shcherbakova","year":"2003","journal-title":"J. Biol. Chem."},{"key":"2024092307420791300_B50","doi-asserted-by":"crossref","first-page":"1763","DOI":"10.1093\/nar\/gkq1034","article-title":"The high fidelity and unique error signature of human DNA polymerase \u03f5","volume":"39","author":"Korona","year":"2011","journal-title":"Nucleic Acids Res."},{"key":"2024092307420791300_B51","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1002\/j.1460-2075.1991.tb07917.x","article-title":"Structural basis for the 3\u2032-5\u2032 Exonuclease activity of Escherichia-coli DNA-polymerase-I - a 2 metal-ion mechanism","volume":"10","author":"Beese","year":"1991","journal-title":"EMBO J."},{"key":"2024092307420791300_B52","doi-asserted-by":"crossref","first-page":"315","DOI":"10.1016\/j.jmb.2012.07.025","article-title":"Molecular recognition of canonical and deaminated bases by P. abyssi family B DNA polymerase","volume":"423","author":"Gouge","year":"2012","journal-title":"J. Mol. Biol."},{"key":"2024092307420791300_B53","doi-asserted-by":"crossref","first-page":"155","DOI":"10.1016\/S0092-8674(00)81647-5","article-title":"Building a replisome from interacting pieces: sliding clamp complexed to a peptide from DNA polymerase and a polymerase editing complex","volume":"99","author":"Shamoo","year":"1999","journal-title":"Cell"},{"key":"2024092307420791300_B54","doi-asserted-by":"crossref","first-page":"1432","DOI":"10.1074\/jbc.M605675200","article-title":"Structural and biochemical investigation of the role in proofreading of a \u03b2 hairpin loop found in the exonuclease domain of a replicative DNA polymerase of the B family","volume":"282","author":"Hogg","year":"2007","journal-title":"J. Biol. 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