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In eukaryotes, recognition of the A-site stop codon and release of the polypeptide are mediated by release factors eRF1 and eRF3, respectively. Translation termination is modulated by other factors which either directly interact with release factors or bind to the E-site and modulate the activity of the peptidyl transferase center. Previous studies suggested that the Saccharomyces cerevisiae ABCF ATPase New1 is involved in translation termination and\/or ribosome recycling, however, the exact function remained unclear. Here, we have applied 5PSeq, single-particle cryo-EM and readthrough reporter assays to provide insight into the biological function of New1. We show that the lack of New1 results in ribosomal stalling at stop codons preceded by a lysine or arginine codon and that the stalling is not defined by the nature of the C-terminal amino acid but rather by the identity of the tRNA isoacceptor in the P-site. Collectively, our results suggest that translation termination is inefficient when ribosomes have specific tRNA isoacceptors in the P-site and that the recruitment of New1 rescues ribosomes at these problematic termination contexts.<\/jats:p>","DOI":"10.1093\/nar\/gkae748","type":"journal-article","created":{"date-parts":[[2024,9,1]],"date-time":"2024-09-01T11:32:16Z","timestamp":1725190336000},"page":"12005-12020","source":"Crossref","is-referenced-by-count":8,"title":["The ABCF ATPase New1 resolves translation termination defects associated with specific tRNAArg and tRNALys isoacceptors in the P site"],"prefix":"10.1093","volume":"52","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0899-974X","authenticated-orcid":false,"given":"Kathryn","family":"Turnbull","sequence":"first","affiliation":[{"name":"Department of Clinical Microbiology, Rigshospitalet , 2200\u00a0 Copenhagen ,","place":["Denmark"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8335-3239","authenticated-orcid":false,"given":"Helge","family":"Paternoga","sequence":"additional","affiliation":[{"name":"Institute for Biochemistry and Molecular Biology, University of Hamburg , Martin-Luther-King-Platz 6, 20146 \u00a0 Hamburg ,","place":["Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Esther","family":"von\u00a0der\u00a0Weth","sequence":"additional","affiliation":[{"name":"Max Planck Institute of Molecular Cell Biology and Genetics , Dresden ,","place":["Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5578-5384","authenticated-orcid":false,"given":"Artyom\u00a0A","family":"Egorov","sequence":"additional","affiliation":[{"name":"Department of Experimental Medicine, University of Lund , 221 84\u00a0 Lund ,","place":["Sweden"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Agnieszka\u00a0A","family":"Pochopien","sequence":"additional","affiliation":[{"name":"Institute for Biochemistry and Molecular Biology, University of Hamburg , Martin-Luther-King-Platz 6, 20146 \u00a0 Hamburg ,","place":["Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yujie","family":"Zhang","sequence":"additional","affiliation":[{"name":"SciLifeLab, Department of Microbiology, Tumor and Cell Biology. Karolinska Institutet , Solna ,","place":["Sweden"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lilit","family":"Nersisyan","sequence":"additional","affiliation":[{"name":"SciLifeLab, Department of Microbiology, Tumor and Cell Biology. Karolinska Institutet , Solna ,","place":["Sweden"]},{"name":"Armenian Bioinformatics Institute , Yerevan ,","place":["Armenia"]},{"name":"Institute of Molecular Biology, National Academy of Sciences of Armenia , Yerevan ,","place":["Armenia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T\u00f5nu","family":"Margus","sequence":"additional","affiliation":[{"name":"Moisavahe 60-28, 50707\u00a0 Tartu ,","place":["Estonia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcus\u00a0J O","family":"Johansson","sequence":"additional","affiliation":[{"name":"Department of Experimental Medicine, University of Lund , 221 84\u00a0 Lund ,","place":["Sweden"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9415-788X","authenticated-orcid":false,"given":"Vicent","family":"Pelechano","sequence":"additional","affiliation":[{"name":"SciLifeLab, Department of Microbiology, Tumor and Cell Biology. Karolinska Institutet , Solna ,","place":["Sweden"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3816-3828","authenticated-orcid":false,"given":"Daniel\u00a0N","family":"Wilson","sequence":"additional","affiliation":[{"name":"Institute for Biochemistry and Molecular Biology, University of Hamburg , Martin-Luther-King-Platz 6, 20146 \u00a0 Hamburg ,","place":["Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2389-5057","authenticated-orcid":false,"given":"Vasili","family":"Hauryliuk","sequence":"additional","affiliation":[{"name":"Department of Experimental Medicine, University of Lund , 221 84\u00a0 Lund ,","place":["Sweden"]},{"name":"University of Tartu, Institute of Technology , 50411\u00a0 Tartu ,","place":["Estonia"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2024,9,1]]},"reference":[{"key":"2024102814484876500_B1","doi-asserted-by":"crossref","first-page":"a013706","DOI":"10.1101\/cshperspect.a013706","article-title":"The elongation, termination, and recycling phases of translation in eukaryotes","volume":"4","author":"Dever","year":"2012","journal-title":"Cold Spring Harb. 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