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By isolating and extending the ELD, we created a novel shuttle peptide, termed S237. While S237 achieved lower delivery of green fluorescent protein, it outperformed S10 at Cas9 RNP delivery to cultured human airway epithelial cells and to pig airway epithelia in vivo, possibly due to its lower net charge. In well-differentiated primary human airway epithelial cell cultures, S237 achieved a 4.6-fold increase in base editor RNP delivery, correcting up to 9.4% of the cystic fibrosis transmembrane conductance regulator (CFTR) R553X allele and restoring CFTR channel function close to non-CF levels. These findings deepen the understanding of peptide-mediated delivery and offer a translational approach for base editor RNP delivery for CF airway disease.<\/jats:p>","DOI":"10.1093\/nar\/gkae819","type":"journal-article","created":{"date-parts":[[2024,9,10]],"date-time":"2024-09-10T17:18:18Z","timestamp":1725988698000},"page":"11911-11925","source":"Crossref","is-referenced-by-count":14,"title":["Amphiphilic shuttle peptide delivers base editor ribonucleoprotein to correct the CFTR R553X mutation in well-differentiated airway epithelial cells"],"prefix":"10.1093","volume":"52","author":[{"given":"Katarina","family":"Kulhankova","sequence":"first","affiliation":[{"name":"Department of Pediatrics, University of Iowa , Iowa City , IA ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna\u00a0X","family":"Cheng","sequence":"additional","affiliation":[{"name":"Feldan Therapeutics , Quebec , Qc ,","place":["Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Soumba","family":"Traore","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, University of Iowa , Iowa City , IA ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maud","family":"Auger","sequence":"additional","affiliation":[{"name":"Feldan Therapeutics , Quebec , Qc ,","place":["Canada"]},{"name":"Department of Chemical Engineering, Laval University , Quebec , Qc ,","place":["Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mia","family":"Pelletier","sequence":"additional","affiliation":[{"name":"Feldan Therapeutics , Quebec , Qc ,","place":["Canada"]},{"name":"Department of Chemical Engineering, Laval University , Quebec , Qc ,","place":["Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maxime","family":"Hervault","sequence":"additional","affiliation":[{"name":"Feldan Therapeutics , Quebec , Qc ,","place":["Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kevin\u00a0D","family":"Wells","sequence":"additional","affiliation":[{"name":"Division of Animal Sciences, Swine Somatic Cell Genome Editing Center, University of Missouri , Columbia , MO ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jonathan\u00a0A","family":"Green","sequence":"additional","affiliation":[{"name":"Division of Animal Sciences, Swine Somatic Cell Genome Editing Center, University of Missouri , Columbia , MO ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Addison","family":"Byrne","sequence":"additional","affiliation":[{"name":"Division of Animal Sciences, Swine Somatic Cell Genome Editing Center, University of Missouri , Columbia , MO ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Benjamin","family":"Nelson","sequence":"additional","affiliation":[{"name":"Division of Animal Sciences, Swine Somatic Cell Genome Editing Center, University of Missouri , Columbia , MO ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mariana","family":"Sponchiado","sequence":"additional","affiliation":[{"name":"Division of Animal Sciences, Swine Somatic Cell Genome Editing Center, University of Missouri , Columbia , MO ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chandra","family":"Boosani","sequence":"additional","affiliation":[{"name":"Division of Animal Sciences, Swine Somatic Cell Genome Editing Center, University of Missouri , Columbia , MO ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Caleb\u00a0S","family":"Heffner","sequence":"additional","affiliation":[{"name":"The Jackson Laboratory, Genetic Resource Science , Bar Harbor , ME ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kathy\u00a0J","family":"Snow","sequence":"additional","affiliation":[{"name":"The Jackson Laboratory, Genetic Resource Science , Bar Harbor , ME ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephen\u00a0A","family":"Murray","sequence":"additional","affiliation":[{"name":"The Jackson Laboratory, Genetic Resource Science , Bar Harbor , ME ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Raul\u00a0A","family":"Villacreses","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, Roy J. and Lucille A. 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