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Despite known mechanisms of the formation of transducing particles, it has not yet been possible to analyze their DNA content at the single-virion level. Using the Staphylococcus epidermidis model and long-read nanopore sequencing, we determined the sequence structure and ratio of phage and PICI genophores, plasmid, and bacterial DNA packaged in normal and small-headed virions. It was shown that the ratios vary mainly depending on the helper phage and the antimicrobial used for induction. When the effect of a strictly lytic phage and its combination with ciprofloxacin on a packaged mobilome was analyzed, no significant increase in mobilome dissemination was observed compared to antibiotics alone. Here, we demonstrate a novel approach for the analysis of transduced bacterial mobilome and show in vitro that lytic phage-based therapeutic strategies do not increase the risk of mobile genetic element transfer.<\/jats:p>","DOI":"10.1093\/nar\/gkaf1165","type":"journal-article","created":{"date-parts":[[2025,11,9]],"date-time":"2025-11-09T23:26:06Z","timestamp":1762730766000},"source":"Crossref","is-referenced-by-count":1,"title":["Virion content unpacked by long-read sequencing: stress-induced changes in transmitted staphylococcal mobilome due to phage-satellite interactions"],"prefix":"10.1093","volume":"53","author":[{"given":"Tibor","family":"Botka","sequence":"first","affiliation":[{"name":"Faculty of Science, Masaryk University Department of Experimental Biology, , Brno 625 00 ,","place":["Czech Republic"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"So\u0148a","family":"Smetanov\u00e1","sequence":"additional","affiliation":[{"name":"Faculty of Science, 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